Rapid identification of Gram‑negative pathogens from positive blood cultures using MALDI‑TOF MS following short‑term incubation on solid media

Neonakis,Ioannis K.; Spandidos,Demetrios A.

doi:10.3892/br.2022.1530

Rapid identification of Gram‑negative pathogens from positive blood cultures using MALDI‑TOF MS following short‑term incubation on solid media

Authors:
- Ioannis K. Neonakis
- Demetrios A. Spandidos
View Affiliations

Affiliations: Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 71201 Heraklion, Greece, Laboratory of Clinical Virology, School of Medicine, University of Crete, 71003 Heraklion, Greece
Published online on: April 8, 2022 https://doi.org/10.3892/br.2022.1530
Article Number: 47
Copyright: © Neonakis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Matrix‑assisted laser desorption ionization‑time of flight mass spectrometry (MALDI‑TOF MS) enables the timely and reliable identification of microbes. The rapid identification of Gram‑negative bacteria (GNB) in bloodstream infections is of critical importance. Several protocols have been proposed for the application of MALDI‑TOF MS on samples from positive blood cultures (BCs) within the same day of BC positivity detection. The majority of these protocols include sample preparation steps with the use of chemicals or repeated centrifugations in order to avoid biases from human cells and proteins from the BC broth. These additional steps increase the hands‑on processing time and the cost of identification. A different approach is to perform a MALDI‑TOF MS analysis using biomass from briefly incubated subcultures on solid media. The present study discusses the findings of previous studies regarding the rapid identification of GNB from positive BC broth using MALDI‑TOF MS following a short‑term incubation period on solid media without any other additional steps or procedures.

View Figures

View References

1	Machen A, Drake T and Wang YF: Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system. PLoS One. 9(e87870)2014.PubMed/NCBI View Article : Google Scholar
2	Freimann S, Shapira M and Athamna A: Serum separator tube method for matrix-assisted laser desorption/ionization time-of-flight analysis. Access Microbiol. 1(e000011)2019.PubMed/NCBI View Article : Google Scholar
3	Monteiro J, Inoue FM, Lobo AP, Sugawara EK, Boaretti FM and Tufik S: Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek^® MS system. J Microbiol Methods. 109:157–159. 2015.PubMed/NCBI View Article : Google Scholar
4	Ha J, Hong SK, Han GH, Kim M, Yong D and Lee K: Same-day identification and antimicrobial susceptibility testing of bacteria in positive blood culture broths using short-term incubation on solid medium with the MicroFlex LT, Vitek-MS, and Vitek2 systems. Ann Lab Med. 38:235–241. 2018.PubMed/NCBI View Article : Google Scholar
5	Idelevich EA, Seifert H, Sundqvist M, Scudeller L, Amit S, Balode A, Bilozor A, Drevinek P, Kocak Tufan Z, Koraqi A, et al: Microbiological diagnostics of bloodstream infections in Europe-an ESGBIES survey. Clin Microbiol Infect. 25:1399–1407. 2019.PubMed/NCBI View Article : Google Scholar
6	Haigh JD, Green IM, Ball D, Eydmann M, Millar M and Wilks M: Rapid identification of bacteria from bioMérieux BacT/ALERT blood culture bottles by MALDI-TOF MS. Br J Biomed Sci. 70:149–155. 2013.PubMed/NCBI View Article : Google Scholar
7	Idelevich EA, Schüle I, Grünastel B, Wüllenweber J, Peters G and Becker K: Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium. Clin Microbiol Infect. 20:1001–1006. 2014.PubMed/NCBI View Article : Google Scholar
8	Bhatti MM, Boonlayangoor S, Beavis KG and Tesic V: Rapid identification of positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry using prewarmed agar plates. J Clin Microbiol. 52:4334–4338. 2014.PubMed/NCBI View Article : Google Scholar
9	Hong SK, Chang BK, Song SH and Kim EC: Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures. Indian J Med Microbiol. 32:419–422. 2014.PubMed/NCBI View Article : Google Scholar
10	Verroken A, Defourny L, Lechgar L, Magnette A, Delmée M and Glupczynski Y: Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture. Eur J Clin Microbiol Infect Dis. 34:405–413. 2015.PubMed/NCBI View Article : Google Scholar
11	Zabbe JB, Zanardo L, Mégraud F and Bessède E: MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles. J Microbiol Methods. 115:45–46. 2015.PubMed/NCBI View Article : Google Scholar
12	Altun O, Botero-Kleiven S, Carlsson S, Ullberg M and Özenci V: Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media. J Med Microbiol. 64:1346–1352. 2015.PubMed/NCBI View Article : Google Scholar
13	Kohlmann R, Hoffmann A, Geis G and Gatermann S: MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures. Int J Med Microbiol. 305:469–479. 2015.PubMed/NCBI View Article : Google Scholar
14	Curtoni A, Cipriani R, Marra ES, Barbui AM, Cavallo R and Costa C: Rapid identification of microorganisms from positive blood culture by MALDI-TOF MS after short-term incubation on solid medium. Curr Microbiol. 74:97–102. 2017.PubMed/NCBI View Article : Google Scholar
15	Froböse NJ, Idelevich EA and Schaumburg F: Short incubation of positive blood cultures on solid media for species identification by MALDI-TOF MS: Which agar is the fastest? Microbiol Spectr. 9(e0003821)2021.PubMed/NCBI View Article : Google Scholar
16	Idelevich EA, Schüle I, Grünastel B, Wüllenweber J, Peters G and Becker K: Acceleration of antimicrobial susceptibility testing of positive blood cultures by inoculation of Vitek 2 cards with briefly incubated solid medium cultures. J Clin Microbiol. 52:4058–4062. 2014.PubMed/NCBI View Article : Google Scholar