Anti‑inflammatory effect of red ginseng marc, Artemisia scoparia, Paeonia japonica and Angelica gigas extract mixture in LPS‑stimulated RAW 264.7 cells

Shin,Jae Young; Kang,Eun Seo; Park,Ji Hyeon; Cho,Byoung Ok; Jang,Seon Il

doi:10.3892/br.2022.1546

Anti‑inflammatory effect of red ginseng marc, Artemisia scoparia, Paeonia japonica and Angelica gigas extract mixture in LPS‑stimulated RAW 264.7 cells

Authors:
- Jae Young Shin
- Eun Seo Kang
- Ji Hyeon Park
- Byoung Ok Cho
- Seon Il Jang
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Affiliations: Institute of Health and Science, Jeonju University, Jeonju‑si, Jeollabuk‑do 55069, Republic of Korea
Published online on: June 2, 2022 https://doi.org/10.3892/br.2022.1546
Article Number: 63
Copyright: © Shin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A normal inflammatory response is essential in protecting the body from foreign substances. However, excessive inflammation contributes to diseases such as oxidative stress, heart disease, and cancer. In this study, we evaluated the anti‑inflammatory effects of RAPA (red ginseng marc, Artemisia scoparia Waldst.et Kit, Paeonia japonica Miyabe & Takeda, and Angelica gigas Nakai extract mixture) in LPS‑stimulated RAW 264.7 cells (macrophages). RAPA suppressed the expression of inflammatory factors such as iNOS and COX‑2 and decreased the production of nitric oxide. In addition, RAPA decreased the expression of the inflammatory cytokines TNF‑α and IL‑6. Furthermore, RAPA inhibited the phosphorylation of MAPKs such as JNK and ERK as well as IκB and NF‑κB. In conclusion, RAPA inhibited production of inflammatory mediators via downregulation of the MAPK and NF‑κB signaling pathways in LPS‑stimulated RAW 264.7 cells. The results of this study demonstrated that RAPA regulates excessive inflammatory responses at the cellular level. Therefore, it is necessary to investigate whether the same effect is observed in vivo through further research.

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