Recruitment of myeloid‑derived suppressor cells and regulatory T‑cells is associated with the occurrence of acute myocardial infarction

Zhang,Mingqiang; Shi,Xiaohu; Zhao,Jingquan; Guo,Wenjia; Zhou,Jie

doi:10.3892/br.2023.1637

Recruitment of myeloid‑derived suppressor cells and regulatory T‑cells is associated with the occurrence of acute myocardial infarction

Authors:
- Mingqiang Zhang
- Xiaohu Shi
- Jingquan Zhao
- Wenjia Guo
- Jie Zhou
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Affiliations: Department of Respiratory and Critical Care Medicine, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102218, P.R. China, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, Department of Respiratory and Critical Care Medicine, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102218, P.R. China, Department of Cardiology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China
Published online on: July 17, 2023 https://doi.org/10.3892/br.2023.1637
Article Number: 55

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Abstract

The roles of myeloid‑derived suppressor cells (MDSCs) and regulatory T‑cells (Tregs) in acute myocardial infarction (AMI) remain elusive. The present study aimed to analyze the proportions of the granulocytic and monocytic populations of MDSCs (G‑MDSCs and M‑MDSCs, respectively), and Tregs in the peripheral blood mononuclear cells (PBMCs) of patients with AMI. The present study recruited 34 patients with AMI and 37 healthy controls without clinical signs of myocardial ischemia. PBMCs were isolated from the peripheral blood samples of patients with AMI within 24 h following admission to the hospital and from those of the healthy controls during a physical examination. Two subsets of MDSCs, G‑MDSCs (CD15⁺CD33⁺CD11b⁺CD14^‑HLA‑DR^low) and M‑MDSCs (CD14⁺CD15^‑CD11b⁺HLA‑DR^low), and Tregs (CD3⁺CD4⁺CD25^highCD127^low T‑cells) in the PBMCs derived from the patients with AMI and healthy controls were analyzed using flow cytometry. The effects of MDSCs derived from patients with AMI on naïve CD4⁺ T‑cells were examined in the co‑culture system. The results revealed that the proportions of G‑MDSCs and M‑MDSCs were higher in the peripheral blood of patients with AMI than in that of the healthy controls. The patients with AMI had significantly higher numbers of programmed death‑ligand (PD‑L)1‑ and PD‑L2‑positive G‑MDSCs and M‑MDSCs compared with the healthy controls (P<0.05). The MDSCs could acquire a granulocytic phenotype following AMI, and the G‑MDSCs and M‑MDSCs would be more likely to express PD‑L2 and PD‑L1, respectively. The ratios of Tregs to CD4⁺ T‑cells and PD‑1⁺ Tregs in the peripheral blood of patients with AMI were significantly higher than those in the healthy controls (P<0.05). The results of flow cytometry demonstrated an increase in the numbers of inducible Tregs in the co‑culture system with the G‑MDSCs derived from patients with AMI compared with the G‑MDSCs derived from the healthy controls (P<0.01). On the whole, the findings presented herein demonstrate the accumulation of MDSCs, and the upregulation of PD‑L1 and PD‑L2 expression on the surface of MDSCs in patients with AMI. MDSCs can induce the expansion of Tregs by binding PD‑1 on the surface of Tregs, thus playing a crucial role in AMI.

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