Antiproliferative activity and apoptosis‑inducing effects of <em>Trametes polyzona</em> polysaccharides against human breast cancer cells

Kurniawan,Budi; Bankeeree,Wichanee; Yanatatsaneejit,Pattamawadee; Prasongsuk,Sehanat

doi:10.3892/br.2023.1665

November-2023 Volume 19 Issue 5

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November-2023 Volume 19 Issue 5

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Article Open Access

Antiproliferative activity and apoptosis‑inducing effects of Trametes polyzona polysaccharides against human breast cancer cells

Authors:
- Budi Kurniawan
- Wichanee Bankeeree
- Pattamawadee Yanatatsaneejit
- Sehanat Prasongsuk
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Affiliations: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, Human Genetics Research Group and Plant Breeding Laboratory, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

Copyright: © Kurniawan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 83
|
Published online on: September 25, 2023

https://doi.org/10.3892/br.2023.1665
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Abstract

Fungal polysaccharides have garnered interest due to their biological activities in terms of anticancer properties and antioxidant activity. The present study aimed to evaluate the anticancer properties and antioxidant activity of a newly isolated white‑rot fungus, Trametes polyzona CU07 from Thailand. Crude T. polyzona polysaccharides (CTPPs) were extracted from mycelia using hot water. The chemical properties, including total carbohydrates, molecular weight and protein content, and Fourier‑transform infrared spectroscopy analysis, were then investigated. The antioxidant activity was determined against the radicals 2,2‑diphenyl‑1‑picrylhydrazyl and 2,2'‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS). The anticancer properties were evaluated in MCF‑7 breast cancer (BC) cells, whereas the 293 cell line was used as a control. The inhibitory effects of CTPPs on viability were determined by MTT assay, followed by BrdU incorporation assay to assess cell proliferation. The induction of apoptosis was determined by flow cytometry. CTPPs were considered polysaccharide‑protein conjugates, which had molecular weights in the range of 0.3‑22,528 kDa. They contained ~50 and 37% carbohydrate and protein, respectively, with glucose as the main monosaccharide component. Notably, CTPPs had high antioxidant activity against ABTS, and had a significant inhibitory effect on the MCF‑7 cell line with a half‑maximal inhibitory concentration value of 0.58 mg/ml. However, they exhibited little effect on the 293 cell line. The BrdU incorporation assay demonstrated that CTPPs inhibited proliferation by ~20% compared with that in untreated cells. CTPPs also induced early‑ and late‑stage apoptosis of MCF‑7 cells. These results indicated that the CTPPs may exhibit potential antiproliferative and antioxidant activity, and apoptosis‑inducing effects against human BC cells.

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