Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
December-2023 Volume 19 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2023 Volume 19 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Helicobacter pylori extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma

  • Authors:
    • Tran Duong Thai
    • Chalida Chuenchom
    • Wachiraporn Donsa
    • Kiatichai Faksri
    • Banchob Sripa
    • Steven W. Edwards
    • Kanin Salao
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand, World Health Organization Collaborating Centre for Research and Control of Opisthorchiasis, Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZX, United Kingdom
    Copyright: © Thai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 89
    |
    Published online on: October 5, 2023
       https://doi.org/10.3892/br.2023.1671
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

H. pylori is a bacterial pathogen infecting over half of the world's population and induces several gastric and extra‑gastric diseases through its various virulence factors, especially cagA. These factors may be released from the bacteria during interactions with host immune cells. Neutrophils play key roles in innate immunity, and their activity is regulated by plasma factors, which can alter how these cells may interact with pathogens. The aim of the present study was to determine whether purified neutrophils could produce reactive oxygen species (ROS), one of the key functions of their anti‑microbial functions, in response to extracts of cagA+ and cagA‑ H. pylori. Extracts from either cagA+ or cagA‑ H. pylori were co‑cultured with human neutrophils in the presence or absence of plasma, and the neutrophil ROS production was measured. In the absence of plasma, extracts from cagA+ and cagA‑ H. pylori did not induce neutrophil ROS production, whereas in the presence of plasma, extracts from both cagA+ and cagA‑ H. pylori‑induced ROS production. Furthermore, when peripheral blood mononuclear cells (PBMCs) were added to the purified neutrophils in the absence of plasma, there was no neutrophil ROS production after challenging with extracts from either cagA+ or cagA‑ H. pylori. Thus, it is suggested that plasma contains immunological components that change the responsiveness of neutrophils, such that when neutrophils encounter the bacterial antigens in H. pylori extracts, they become activated and produce ROS. This study also revealed a potential novel immunopathogenic pathway by which cagA activation of neutrophils contributed to inflammatory damage.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Baj J, Forma A, Sitarz M, Portincasa P, Garruti G, Krasowska D and Maciejewski R: Helicobacter pylori virulence factors-mechanisms of bacterial pathogenicity in the gastric microenvironment. Cells. 10(27)2020.PubMed/NCBI View Article : Google Scholar

2 

Noto JM and Peek RM Jr: The helicobacter pylori cag Pathogenicity Island. Methods Mol Biol. 921:41–50. 2012.PubMed/NCBI View Article : Google Scholar

3 

Hatakeyama M: Structure and function of Helicobacter pylori CagA, the first-identified bacterial protein involved in human cancer. Proc Jpn Acad Ser B Phys Biol Sci. 93:196–219. 2017.PubMed/NCBI View Article : Google Scholar

4 

Yamaoka Y, Kita M, Kodama T, Sawai N and Imanishi J: Helicobacter pylori cagA gene and expression of cytokine messenger RNA in gastric mucosa. Gastroenterology. 110:1744–1752. 1996.PubMed/NCBI View Article : Google Scholar

5 

Crabtree JE, Farmery SM, Lindley IJ, Figura N, Peichl P and Tompkins DS: CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines. J Clin Pathol. 47:945–950. 1994.PubMed/NCBI View Article : Google Scholar

6 

Tohidpour A: CagA-mediated pathogenesis of Helicobacter pylori. Microb Pathog. 93:44–55. 2016.PubMed/NCBI View Article : Google Scholar

7 

Wright HL, Moots RJ and Edwards SW: The multifactorial role of neutrophils in rheumatoid arthritis. Nat Rev Rheumatol. 10:593–601. 2014.PubMed/NCBI View Article : Google Scholar

8 

Salao K, Spofford EM, Price C, Mairiang E, Suttiprapa S, Wright HL, Sripa B and Edwards SW: Enhanced neutrophil functions during Opisthorchis viverrini infections and correlation with advanced periductal fibrosis. Int J Parasitol. 50:145–152. 2020.PubMed/NCBI View Article : Google Scholar

9 

Han L, Shu X and Wang J: Helicobacter pylori-mediated oxidative stress and gastric diseases: A Review. Front Microbiol. 13(811258)2022.PubMed/NCBI View Article : Google Scholar

10 

Psychogios N, Hau DD, Peng J, Guo AC, Mandal R, Bouatra S, Sinelnikov I, Krishnamurthy R, Eisner R, Gautam B, et al: The human serum metabolome. PLoS One. 6(e16957)2011.PubMed/NCBI View Article : Google Scholar

11 

Perez-Figueroa E, Torres J, Sanchez-Zauco N, Contreras-Ramos A, Alvarez-Arellano L and Maldonado-Bernal C: Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection. Innate Immun. 22:103–112. 2016.PubMed/NCBI View Article : Google Scholar

12 

Faass L, Hauke M, Stein SC and Josenhans C: Innate immune activation and modulatory factors of Helicobacter pylori towards phagocytic and nonphagocytic cells. Curr Opin Immunol. 82(102301)2023.PubMed/NCBI View Article : Google Scholar

13 

Pham KT, Weiss E, Jimenez Soto LF, Breithaupt U, Haas R and Fischer W: CagI is an essential component of the Helicobacter pylori Cag type IV secretion system and forms a complex with CagL. PLoS One. 7(e35341)2012.PubMed/NCBI View Article : Google Scholar

14 

Almaraz-Arreortua A, Sosa-Luis SA, Rios-Rios WJ, Romero-Tlalolini MLÁ, Aguilar-Ruiz SR, Baltiérrez-Hoyos R and Torres Aguilar H: Morphological and compositional analysis of neutrophil extracellular traps induced by microbial and chemical stimuli. J Vis Exp. 2022.PubMed/NCBI View Article : Google Scholar

15 

Parsonnet J, Friedman GD, Orentreich N and Vogelman H: Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut. 40:297–301. 1997.PubMed/NCBI View Article : Google Scholar

16 

Seyoum M, Enawgaw B and Melku M: Human blood platelets and viruses: Defense mechanism and role in the removal of viral pathogens. Thromb J. 16(16)2018.PubMed/NCBI View Article : Google Scholar

17 

Lawton JW, Robinson JP and Till GO: The effect of intravenous immunoglobulin on the in vitro function of human neutrophils. Immunopharmacology. 18:97–105. 1989.PubMed/NCBI View Article : Google Scholar

18 

Matsuo H, Itoh H, Kitamura N, Kamikubo Y, Higuchi T, Shiga S, Ichiyama S, Kondo T, Takaori-Kondo A and Adachi S: Intravenous immunoglobulin enhances the killing activity and autophagy of neutrophils isolated from immunocompromised patients against multidrug-resistant bacteria. Biochem Biophys Res Commun. 464:94–99. 2015.PubMed/NCBI View Article : Google Scholar

19 

Halbgebauer R, Schmidt CQ, Karsten CM, Ignatius A and Huber-Lang M: Janus face of complement-driven neutrophil activation during sepsis. Semin Immunol. 37:12–20. 2018.PubMed/NCBI View Article : Google Scholar

20 

He Y, Wang C, Zhang X, Lu X, Xing J, Lv J, Guo M, Huo X, Liu X, Lu J, et al: Sustained exposure to helicobacter pylori lysate inhibits apoptosis and autophagy of gastric epithelial cells. Front Oncol. 10(581364)2020.PubMed/NCBI View Article : Google Scholar

21 

Ki MR, Goo MJ, Park JK, Hong IH, Ji AR, Han SY, You SY, Lee EM, Kim AY, Park SJ, et al: Helicobacter pylori accelerates hepatic fibrosis by sensitizing transforming growth factor-β1-induced inflammatory signaling. Lab Invest. 90:1507–1516. 2010.PubMed/NCBI View Article : Google Scholar

22 

Skene C, Young A, Every A and Sutton P: Helicobacter pylori flagella: Antigenic profile and protective immunity. FEMS Immunol Med Microbiol. 50:249–256. 2007.PubMed/NCBI View Article : Google Scholar

23 

Whitmore LC, Weems MN and Allen LH: Cutting Edge: Helicobacter pylori Induces nuclear hypersegmentation and subtype differentiation of human neutrophils in vitro. J Immunol. 198:1793–1797. 2017.PubMed/NCBI View Article : Google Scholar

24 

Gros B, Gomez Perez A, Pleguezuelo M, Serrano Ruiz FJ, de la Mata M and Rodriguez-Peralvarez M: Helicobacter species and hepato-biliary tract malignancies: A systematic review and meta-analysis. Cancers (Basel). 15(595)2023.PubMed/NCBI View Article : Google Scholar

25 

Osaki T, Lin Y, Sasahira N, Ueno M, Yonezawa H, Hojo F, Okuda M, Matsuyama M, Sasaki T, Kobayashi S, et al: Prevalence estimates of Helicobacter species infection in pancreatic and biliary tract cancers. Helicobacter. 27(e12866)2022.PubMed/NCBI View Article : Google Scholar

26 

Zhou D, Wang JD, Weng MZ, Zhang Y, Wang XF, Gong W and Quan ZW: Infections of Helicobacter spp. in the biliary system are associated with biliary tract cancer: A meta-analysis. Eur J Gastroenterol Hepatol. 25:447–454. 2013.PubMed/NCBI View Article : Google Scholar

27 

Aviles-Jimenez F, Guitron A, Segura-Lopez F, Méndez-Tenorio A, Iwai S, Hernández-Guerrero A and Torres J: Microbiota studies in the bile duct strongly suggest a role for Helicobacter pylori in extrahepatic cholangiocarcinoma. Clin Microbiol Infect. 22:178 e11–178 e22. 2016.PubMed/NCBI View Article : Google Scholar

28 

Deenonpoe R, Mairiang E, Mairiang P, Pairojkul C, Chamgramol Y, Rinaldi G, Loukas A, Brindley PJ and Sripa B: Elevated prevalence of Helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease. Sci Rep. 7(42744)2017.PubMed/NCBI View Article : Google Scholar

29 

Deenonpoe R, Chomvarin C, Pairojkul C, Chamgramol Y, Loukas A, Brindley PJ and Sripa B: The carcinogenic liver fluke Opisthorchis viverrini is a reservoir for species of Helicobacter. Asian Pac J Cancer Prev. 16:1751–1758. 2015.PubMed/NCBI View Article : Google Scholar

30 

Mairiang E, Laha T, Kaewkes S, Loukas A, Bethony J, Brindley PJ and Sripa B: Hepatobiliary morbidities detected by ultrasonography in Opisthorchis viverrini-infected patients before and after praziquantel treatment: A five-year follow up study. Acta Trop. 217(105853)2021.PubMed/NCBI View Article : Google Scholar

31 

Phung HTT, Deenonpoe R, Suttiprapa S, Mairiang E, Edwards SW and Sripa B: Persistent advanced periductal fibrosis is associated with cagA-positive Helicobacter pylori infection in post-praziquantel treatment of opisthorchiasis. Helicobacter. 27(e12897)2022.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Thai TD, Chuenchom C, Donsa W, Faksri K, Sripa B, Edwards SW and Salao K: <em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma. Biomed Rep 19: 89, 2023.
APA
Thai, T.D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S.W., & Salao, K. (2023). <em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma. Biomedical Reports, 19, 89. https://doi.org/10.3892/br.2023.1671
MLA
Thai, T. D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S. W., Salao, K."<em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma". Biomedical Reports 19.6 (2023): 89.
Chicago
Thai, T. D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S. W., Salao, K."<em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma". Biomedical Reports 19, no. 6 (2023): 89. https://doi.org/10.3892/br.2023.1671
Copy and paste a formatted citation
x
Spandidos Publications style
Thai TD, Chuenchom C, Donsa W, Faksri K, Sripa B, Edwards SW and Salao K: <em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma. Biomed Rep 19: 89, 2023.
APA
Thai, T.D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S.W., & Salao, K. (2023). <em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma. Biomedical Reports, 19, 89. https://doi.org/10.3892/br.2023.1671
MLA
Thai, T. D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S. W., Salao, K."<em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma". Biomedical Reports 19.6 (2023): 89.
Chicago
Thai, T. D., Chuenchom, C., Donsa, W., Faksri, K., Sripa, B., Edwards, S. W., Salao, K."<em>Helicobacter pylori</em> extract induces purified neutrophils to produce reactive oxygen species only in the presence of plasma". Biomedical Reports 19, no. 6 (2023): 89. https://doi.org/10.3892/br.2023.1671
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team