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Article Open Access

10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells

  • Authors:
    • María Elizabeth Preciado‑Ortiz
    • Erika Martinez‑Lopez
    • Roberto Rodriguez‑Echevarría
    • Mariana Perez‑Robles
    • Gildardo Gembe‑Olivarez
    • Juan José Rivera‑Valdés
  • View Affiliations / Copyright

    Affiliations: Institute of Translational Nutrigenetics and Nutrigenomics, Department of Molecular Biology and Genomics, University Center of Health Sciences, University of Guadalajara, Guadalajara, Jalisco 44340, Mexico
    Copyright: © Preciado‑Ortiz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 105
    |
    Published online on: November 1, 2023
       https://doi.org/10.3892/br.2023.1687
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Abstract

Obesity is defined as excessive fat accumulation that can be detrimental to health and currently affects a large part of the global population. Obesity arises from excessive energy intake along with a sedentary lifestyle and leads to adipocytes with aggravated hypertrophy. Strategies have been designed to prevent and treat obesity. Nutrigenomics may serve a role in prevention of obesity using bioactive compounds present in certain foods with anti‑obesogenic effects. Ginger (Zingiber officinale Roscoe) contains gingerols, key bioactive compounds that inhibit hypertrophy and hyperplasia of adipocytes. The present study aimed to evaluate the antiadipogenic activity of 10‑gingerol (10‑G) in the 3T3‑L1 cell line. Three study groups were formed: Negative (3T3‑L1 preadipocytes) and positive control (mature 3T3‑L1 adipocytes) and 10‑G (3T3‑L1 preadipocytes stimulated with 10‑G during adipogenic differentiation). Cell viability and lipid content were evaluated by MTT assay and Oil Red O staining, respectively. mRNA expression of CCAAT enhancer‑binding protein α (C/ebpα), peroxisome proliferator‑activated receptor γ (Pparγ), mechanistic target of rapamycin complex (Mtor), sterol regulatory element binding transcription factor 1 (Srebf1), acetyl‑coenzyme A carboxylase (Acaca), fatty acid binding protein 4 (Fabp4), and 18S rRNA (Rn18s), was quantified by quantitative PCR. The protein expression of C/EPBα was analyzed by western blot. In the 10‑G group, lipid content was decreased by 28.83% (P<0.0001) compared with the positive control; notably, cell viability was not affected (P=0.336). The mRNA expression in the 10‑G group was higher for C/ebpα (P<0.001) and lower for Acaca (P<0.001), Fabp4 (P<0.001), Mtor (P<0.0001) and Srebf1 (P<0.0001) compared with the positive control group, while gene expression of Pparγ did not present significant changes. The presence of 10‑G notably decreased C/EBPα protein levels in 3T3‑L1 adipocytes. In summary, the antiadipogenic effect of 10‑G during the differentiation of 3T3‑L1 cells into adipocytes may be explained by mRNA downregulation of adipogenic transcriptional factors and lipid metabolism‑associated genes.
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Copy and paste a formatted citation
Spandidos Publications style
Preciado‑Ortiz ME, Martinez‑Lopez E, Rodriguez‑Echevarría R, Perez‑Robles M, Gembe‑Olivarez G and Rivera‑Valdés JJ: 10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells. Biomed Rep 19: 105, 2023.
APA
Preciado‑Ortiz, M.E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., & Rivera‑Valdés, J.J. (2023). 10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells. Biomedical Reports, 19, 105. https://doi.org/10.3892/br.2023.1687
MLA
Preciado‑Ortiz, M. E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., Rivera‑Valdés, J. J."10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells". Biomedical Reports 19.6 (2023): 105.
Chicago
Preciado‑Ortiz, M. E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., Rivera‑Valdés, J. J."10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells". Biomedical Reports 19, no. 6 (2023): 105. https://doi.org/10.3892/br.2023.1687
Copy and paste a formatted citation
x
Spandidos Publications style
Preciado‑Ortiz ME, Martinez‑Lopez E, Rodriguez‑Echevarría R, Perez‑Robles M, Gembe‑Olivarez G and Rivera‑Valdés JJ: 10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells. Biomed Rep 19: 105, 2023.
APA
Preciado‑Ortiz, M.E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., & Rivera‑Valdés, J.J. (2023). 10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells. Biomedical Reports, 19, 105. https://doi.org/10.3892/br.2023.1687
MLA
Preciado‑Ortiz, M. E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., Rivera‑Valdés, J. J."10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells". Biomedical Reports 19.6 (2023): 105.
Chicago
Preciado‑Ortiz, M. E., Martinez‑Lopez, E., Rodriguez‑Echevarría, R., Perez‑Robles, M., Gembe‑Olivarez, G., Rivera‑Valdés, J. J."10‑Gingerol, a novel ginger compound, exhibits antiadipogenic effects without compromising cell viability in 3T3‑L1 cells". Biomedical Reports 19, no. 6 (2023): 105. https://doi.org/10.3892/br.2023.1687
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