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Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes

  • Authors:
    • Nivethasri Lakshmana Perumal
    • Sung Kuk Do
    • Jong-Soon Choi
    • Je-Ho Lee
    • Gyung-Tae Ban
    • Gyuri Kim
    • Amila Mufida
    • Hwa Seung Yoo
    • Byeong-Churl Jang
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Medicine, College of Medicine, Keimyung University, Daegu 42601, Republic of Korea, College of Korean Medicine, Daejeon University, Daejeon 34520, Republic of Korea, Research Center for Materials Analysis, Korea Basic Science Institute, Daejeon 34133, Republic of Korea, Geron Biotech Ltd., Daejeon 34133, Republic of Korea
    Copyright: © Perumal et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 4
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    Published online on: November 15, 2023
       https://doi.org/10.3892/br.2023.1692
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Abstract

Nutmeg is the seed derived from Myristica fragrans. Nutmeg seeds contain alkylbenzene derivatives such as myristicin, which are toxic to the human organism, and lignan compounds such as nectandrin B, which possess anti‑aging and anti‑diabetic properties. However, the anti‑adipogenic, prolipolytic and anti‑inflammatory effects of lignan‑enriched nutmeg extract (LNX) on preadipocytes remain unclear. In the present study, the effects of LNX on lipid accumulation, glycerol release and inflammatory cyclooxygenase‑2 (COX‑2) expression in differentiated 3T3‑L1 preadipocytes were investigated. Oil red O staining demonstrated that treatment with LNX resulted in a concentration‑dependent reduction in lipid accumulation in differentiating 3T3‑L1 preadipocytes without affecting cell growth. Mechanistically, LNX treatment at 6 µg/ml led to a reduction in phosphorylation levels of signal transducer and activator of transcription 3 (STAT3), whereas it did not influence the peroxisome proliferator‑activated receptor gamma (PPAR‑γ) and CCAAT enhancer binding protein alpha (C/EBP‑α) expression levels during 3T3‑L1 preadipocyte differentiation. In addition, LNX treatment at 6 µg/ml led to a decrease in fatty acid synthase (FAS) expression levels on day (D) 2, but not D5 and D8, during preadipocyte differentiation. Treatment with LNX at 6 µg/ml did not affect the expression levels of perilipin A during preadipocyte differentiation. In differentiated 3T3‑L1 adipocytes, LNX treatment at 6 µg/ml did not stimulate glycerol release and hormone‑sensitive lipase phosphorylation, which are known lipolysis hallmarks. Furthermore, LNX treatment at the doses tested had no effect on tumor necrosis factor alpha‑induced COX‑2 expression in 3T3‑L1 preadipocytes. Collectively, these results demonstrated that LNX has an anti‑adipogenic effect on differentiating 3T3‑L1 preadipocytes, which is mediated by the downregulation of STAT3 phosphorylation and FAS expression.
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Copy and paste a formatted citation
Spandidos Publications style
Perumal NL, Do SK, Choi J, Lee J, Ban G, Kim G, Mufida A, Yoo H and Jang B: Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes. Biomed Rep 20: 4, 2024.
APA
Perumal, N.L., Do, S.K., Choi, J., Lee, J., Ban, G., Kim, G. ... Jang, B. (2024). Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes. Biomedical Reports, 20, 4. https://doi.org/10.3892/br.2023.1692
MLA
Perumal, N. L., Do, S. K., Choi, J., Lee, J., Ban, G., Kim, G., Mufida, A., Yoo, H., Jang, B."Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes". Biomedical Reports 20.1 (2024): 4.
Chicago
Perumal, N. L., Do, S. K., Choi, J., Lee, J., Ban, G., Kim, G., Mufida, A., Yoo, H., Jang, B."Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes". Biomedical Reports 20, no. 1 (2024): 4. https://doi.org/10.3892/br.2023.1692
Copy and paste a formatted citation
x
Spandidos Publications style
Perumal NL, Do SK, Choi J, Lee J, Ban G, Kim G, Mufida A, Yoo H and Jang B: Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes. Biomed Rep 20: 4, 2024.
APA
Perumal, N.L., Do, S.K., Choi, J., Lee, J., Ban, G., Kim, G. ... Jang, B. (2024). Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes. Biomedical Reports, 20, 4. https://doi.org/10.3892/br.2023.1692
MLA
Perumal, N. L., Do, S. K., Choi, J., Lee, J., Ban, G., Kim, G., Mufida, A., Yoo, H., Jang, B."Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes". Biomedical Reports 20.1 (2024): 4.
Chicago
Perumal, N. L., Do, S. K., Choi, J., Lee, J., Ban, G., Kim, G., Mufida, A., Yoo, H., Jang, B."Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes". Biomedical Reports 20, no. 1 (2024): 4. https://doi.org/10.3892/br.2023.1692
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