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Evaluation of P53 immunostaining in patients with cutaneous melanoma

  • Authors:
    • Jiraroch Meevassana
    • Chanya Mittrakulkij
    • Ponteera Toworrakul
    • Wantamol Saensuk
    • Supitcha Kamolratanakul
    • Tippawan Siritientong
    • Komkrit Ruangritchankul
    • Nakarin Kitkumthorn
  • View Affiliations / Copyright

    Affiliations: Center of Excellence in Burn and Wound Care, Chulalongkorn University, Bangkok 10330, Thailand, Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand, Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand
    Copyright: © Meevassana et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 8
    |
    Published online on: November 22, 2023
       https://doi.org/10.3892/br.2023.1696
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Abstract

P53 is a tumor suppressor gene that is mutated in numerous types of cancer. The aim of the present study was to determine the frequency of this mutation in cutaneous melanomas and to conduct clinicopathological characteristics and clinical outcome association analyses with the P53 mutation. P53 immunohistochemical staining was used as a surrogate marker for P53 mutation analysis to assess P53 status. In the present study, 50 pathological samples of cutaneous melanoma from 2012 to 2018 at Chulalongkorn University (Bangkok, Thailand), were subjected to anti‑P53 immunohistochemistry, followed by an examination of the association between P53 statuses and clinical and pathological characteristics, along with clinical outcomes. A positive staining for anti‑P53 antibody was detected in 30% of patients (15/50) with cutaneous melanomas. Positivity was significantly associated with female sex, nodular histological subtype and Breslow level 4. Cox regression analysis revealed that an age >65.5 years and Breslow grade 4 disease were associated with mortality. The Kaplan‑Meier curve revealed a shorter duration of recurrence time in the P53 mutation than P53 wild type. In the present study, P53 mutations in specific cases of cutaneous melanoma were identified. Notably, patients who were older and/or had a Breslow score of 4 exhibited an increased risk of mortality. These findings suggested the potential involvement of P53 mutations in cutaneous melanoma, highlighting the necessity for further investigations to improve understanding of their roles.
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Copy and paste a formatted citation
Spandidos Publications style
Meevassana J, Mittrakulkij C, Toworrakul P, Saensuk W, Kamolratanakul S, Siritientong T, Ruangritchankul K and Kitkumthorn N: Evaluation of P53 immunostaining in patients with cutaneous melanoma. Biomed Rep 20: 8, 2024.
APA
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T. ... Kitkumthorn, N. (2024). Evaluation of P53 immunostaining in patients with cutaneous melanoma. Biomedical Reports, 20, 8. https://doi.org/10.3892/br.2023.1696
MLA
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T., Ruangritchankul, K., Kitkumthorn, N."Evaluation of P53 immunostaining in patients with cutaneous melanoma". Biomedical Reports 20.1 (2024): 8.
Chicago
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T., Ruangritchankul, K., Kitkumthorn, N."Evaluation of P53 immunostaining in patients with cutaneous melanoma". Biomedical Reports 20, no. 1 (2024): 8. https://doi.org/10.3892/br.2023.1696
Copy and paste a formatted citation
x
Spandidos Publications style
Meevassana J, Mittrakulkij C, Toworrakul P, Saensuk W, Kamolratanakul S, Siritientong T, Ruangritchankul K and Kitkumthorn N: Evaluation of P53 immunostaining in patients with cutaneous melanoma. Biomed Rep 20: 8, 2024.
APA
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T. ... Kitkumthorn, N. (2024). Evaluation of P53 immunostaining in patients with cutaneous melanoma. Biomedical Reports, 20, 8. https://doi.org/10.3892/br.2023.1696
MLA
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T., Ruangritchankul, K., Kitkumthorn, N."Evaluation of P53 immunostaining in patients with cutaneous melanoma". Biomedical Reports 20.1 (2024): 8.
Chicago
Meevassana, J., Mittrakulkij, C., Toworrakul, P., Saensuk, W., Kamolratanakul, S., Siritientong, T., Ruangritchankul, K., Kitkumthorn, N."Evaluation of P53 immunostaining in patients with cutaneous melanoma". Biomedical Reports 20, no. 1 (2024): 8. https://doi.org/10.3892/br.2023.1696
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