Open Access

Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

  • Authors:
    • Anne V. Yagolovich
    • Andrey N. Kuskov
    • Pavel P. Kulikov
    • Dmitry V. Bagrov
    • Polina A. Petrova
    • Ekaterina V. Kukovyakina
    • Alina A. Isakova
    • Irina I. Khan
    • Vadim S. Pokrovsky
    • Alexander E. Nosyrev
    • Polyxeni C. Stamati
    • Elena A. Markvicheva
    • Marine E. Gasparian
    • Demetrios A. Spandidos
    • Aristidis M. Tsatsakis
  • View Affiliations

  • Published online on: January 17, 2024     https://doi.org/10.3892/br.2024.1725
  • Article Number: 37
  • Copyright: © Yagolovich et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proteasome inhibitor bortezomib is an anticancer agent approved for treatment of multiple myeloma and mantle cell lymphoma. However, its application in other types of cancer, primarily in solid tumors, is limited due to poor pharmacokinetics, inefficient tissue penetration, low stability and frequent adverse effects. In the present study, a novel micellar nano‑scaled delivery system was manufactured, composed of amphiphilic poly(N‑vinylpyrrolidone) nanoparticles loaded with bortezomib. Similar nanoparticles loaded with prothionamide, a drug without anticancer effect, were used as control. The size and zeta potential of the obtained polymeric micelles were measured by dynamic light scattering. Bortezomib‑loaded micelles exhibited significant cytotoxic activity in vitro in monolayer tumor cell cultures (IC50 ~6.5 µg/ml) and in 3D multicellular tumor spheroids (IC50 ~8.5 µg/ml) of human glioblastoma cell lines U87 and T98G. Additionally, the toxic effects in vivo were studied in zebrafish Danio rerio embryos, with an estimated 50% lethal concentration of 0.1 mg/ml. Considering that bortezomib and other molecules from the class of proteasome inhibitors are potent antitumor agents, nanodelivery approach can help reduce adverse effects and expand the range of its applications for treatment of various oncological diseases.
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March-2024
Volume 20 Issue 3

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Spandidos Publications style
Yagolovich AV, Kuskov AN, Kulikov PP, Bagrov DV, Petrova PA, Kukovyakina EV, Isakova AA, Khan II, Pokrovsky VS, Nosyrev AE, Nosyrev AE, et al: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos. Biomed Rep 20: 37, 2024
APA
Yagolovich, A.V., Kuskov, A.N., Kulikov, P.P., Bagrov, D.V., Petrova, P.A., Kukovyakina, E.V. ... Tsatsakis, A.M. (2024). Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos. Biomedical Reports, 20, 37. https://doi.org/10.3892/br.2024.1725
MLA
Yagolovich, A. V., Kuskov, A. N., Kulikov, P. P., Bagrov, D. V., Petrova, P. A., Kukovyakina, E. V., Isakova, A. A., Khan, I. I., Pokrovsky, V. S., Nosyrev, A. E., Stamati, P. C., Markvicheva, E. A., Gasparian, M. E., Spandidos, D. A., Tsatsakis, A. M."Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos". Biomedical Reports 20.3 (2024): 37.
Chicago
Yagolovich, A. V., Kuskov, A. N., Kulikov, P. P., Bagrov, D. V., Petrova, P. A., Kukovyakina, E. V., Isakova, A. A., Khan, I. I., Pokrovsky, V. S., Nosyrev, A. E., Stamati, P. C., Markvicheva, E. A., Gasparian, M. E., Spandidos, D. A., Tsatsakis, A. M."Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos". Biomedical Reports 20, no. 3 (2024): 37. https://doi.org/10.3892/br.2024.1725