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Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases

  • Authors:
    • Dana Dlouha
    • Pavlina Huckova
    • Eva Rohlova
    • Jevgenija Vymetalova
    • Sarka Novakova
    • Jaroslav A. Hubacek
  • View Affiliations / Copyright

    Affiliations: Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, 14021 Prague, Czech Republic, Cardio Center, Institute for Clinical and Experimental Medicine, 14021 Prague, Czech Republic
    Copyright: © Dlouha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 50
    |
    Published online on: January 29, 2024
       https://doi.org/10.3892/br.2024.1738
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Abstract

The current standard for graft rejection surveillance is endomyocardial biopsy (EMB), an invasive procedure with rare but potentially serious complications. Detection of circulating donor‑derived cell‑free DNA (ddcfDNA) is an option for noninvasive monitoring of graft injury and rejection. A 63‑year‑old man and a 65‑year‑old woman were monitored by EMB for allograft rejection. A total of 48 single‑nucleotide polymorphisms with a minor allele frequency range of 0.4‑0.5 were screened to distinguish donor and recipient DNA based on homozygosity, and digital droplet PCR was used to analyze ddcfDNA concentrations. Both subjects suffered rejection within the first 6 months after transplantation. The maximal ddcfDNA level of 270 copies (cp)/ml during EMB‑confirmed acute cellular rejection (ACR; mild grade 1R/2, patient 1), and the maximal concentration of 1,846 cp/ml in the case of EMB‑confirmed antibody‑mediated rejection (AMR; grade 1+; patient 2), was detected. Individual monitoring of ddcfDNA dynamics from the 1st to the 6th month posttransplant reflected cardiac graft injury in patients suffering ACR or AMR, meaning that ddcfDNA may serve as a noninvasive biomarker.
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Copy and paste a formatted citation
Spandidos Publications style
Dlouha D, Huckova P, Rohlova E, Vymetalova J, Novakova S and Hubacek JA: Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases. Biomed Rep 20: 50, 2024.
APA
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., & Hubacek, J.A. (2024). Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases. Biomedical Reports, 20, 50. https://doi.org/10.3892/br.2024.1738
MLA
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., Hubacek, J. A."Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases". Biomedical Reports 20.3 (2024): 50.
Chicago
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., Hubacek, J. A."Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases". Biomedical Reports 20, no. 3 (2024): 50. https://doi.org/10.3892/br.2024.1738
Copy and paste a formatted citation
x
Spandidos Publications style
Dlouha D, Huckova P, Rohlova E, Vymetalova J, Novakova S and Hubacek JA: Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases. Biomed Rep 20: 50, 2024.
APA
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., & Hubacek, J.A. (2024). Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases. Biomedical Reports, 20, 50. https://doi.org/10.3892/br.2024.1738
MLA
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., Hubacek, J. A."Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases". Biomedical Reports 20.3 (2024): 50.
Chicago
Dlouha, D., Huckova, P., Rohlova, E., Vymetalova, J., Novakova, S., Hubacek, J. A."Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases". Biomedical Reports 20, no. 3 (2024): 50. https://doi.org/10.3892/br.2024.1738
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