Open Access

Extracellular miRNAs in the serum and feces of mice exposed to high‑dose radiation

  • Authors:
    • Mitsuru Chiba
    • Haruka Uehara
    • Haruka Kuwata
    • Ikumi Niiyama
  • View Affiliations

  • Published online on: February 1, 2024     https://doi.org/10.3892/br.2024.1744
  • Article Number: 55
  • Copyright: © Chiba et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exposure to high‑dose radiation causes life‑threatening intestinal damage. Histopathology is the most accurate method of judging the extent of intestinal damage following death. However, it is difficult to predict the extent of intestinal damage. The present study investigated extracellular microRNAs (miRNAs or miRs) in serum and feces using a radiation‑induced intestinal injury mouse model. A peak of 25‑200 nucleotide small RNAs was detected in mouse serum and feces by bioanalyzer, indicating the presence of miRNAs. Microarray analysis detected four miRNAs expressed in the small intestine and increased by >2‑fold in serum and 19 in feces following 10 Gy radiation exposure. Increased miR‑375‑3p in both serum and feces suggests leakage due to radiation‑induced intestinal injury and may be a candidate for high‑dose radiation biomarkers.
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March-2024
Volume 20 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Copy and paste a formatted citation
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Spandidos Publications style
Chiba M, Uehara H, Kuwata H and Niiyama I: Extracellular miRNAs in the serum and feces of mice exposed to high‑dose radiation. Biomed Rep 20: 55, 2024
APA
Chiba, M., Uehara, H., Kuwata, H., & Niiyama, I. (2024). Extracellular miRNAs in the serum and feces of mice exposed to high‑dose radiation. Biomedical Reports, 20, 55. https://doi.org/10.3892/br.2024.1744
MLA
Chiba, M., Uehara, H., Kuwata, H., Niiyama, I."Extracellular miRNAs in the serum and feces of mice exposed to high‑dose radiation". Biomedical Reports 20.3 (2024): 55.
Chicago
Chiba, M., Uehara, H., Kuwata, H., Niiyama, I."Extracellular miRNAs in the serum and feces of mice exposed to high‑dose radiation". Biomedical Reports 20, no. 3 (2024): 55. https://doi.org/10.3892/br.2024.1744