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Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells

  • Authors:
    • Fuki Sasaki
    • Hironori Yoshino
    • Ayumu Kusuhara
    • Kota Sato
    • Eichi Tsuruga
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Science, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan, Department of Radiological Technology, School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan
    Copyright: © Sasaki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 70
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    Published online on: March 5, 2024
       https://doi.org/10.3892/br.2024.1758
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Abstract

In 2012, the threshold radiation dose (0.5 Gy) for cardiovascular and cerebrovascular diseases was revised, and this threshold dose may be exceeded during procedures involving radiation such as interventional radiology. Therefore, in addition to regulating radiation dose, it is necessary to develop strategies to prevent and mitigate the development of cardiovascular disease. Cellular senescence is irreversible arrest of cell proliferation. Although cellular senescence is one of the mechanisms for suppressing cancer, it also has adverse effects. For example, senescence of vascular endothelial cells is involved in development of vascular disorders. However, the mechanisms underlying induction of cellular senescence are not fully understood. Therefore, the present study explored the factors involved in the radiation‑induced senescence in human umbilical vein endothelial cells (HUVECs). The present study reanalyzed the gene expression data of senescent normal human endothelial cells and fibroblast after irradiation (NCBI Gene Expression Omnibus accession no. GSE130727) and microarray data of HUVECs 24 h after irradiation (NCBI Gene Expression Omnibus accession no. GSE76484). Numerous genes related to viral infection and inflammation were upregulated in radiation‑induced senescent cells. In addition, the gene group involved in the retinoic acid‑inducible gene‑I (RIG‑I)‑like receptor (RLR) signaling pathway, which plays an important role to induce anti‑viral response, was altered in irradiated HUVECs. Therefore, to investigate the involvement of RIG‑I and melanoma differentiation‑associated gene 5 (MDA5), which are RLRs, in radiation‑induced senescence of HUVECs, the protein expression of RIG‑I and MDA5 and the activity of senescence‑associated β‑galactosidase (SA‑β‑gal), a representative senescence marker, were analyzed. Of note, knockdown of RIG‑I in HUVECs significantly decreased radiation‑increased proportion of cells with high SA‑β‑gal activity (i.e., senescent cells), whereas this phenomenon was not observed in MDA5‑knockdown cells. Taken together, the present results suggested that RIG‑I, but not MDA5, was associated with radiation‑induced senescence in HUVECs.
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Copy and paste a formatted citation
Spandidos Publications style
Sasaki F, Yoshino H, Kusuhara A, Sato K and Tsuruga E: Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells. Biomed Rep 20: 70, 2024.
APA
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., & Tsuruga, E. (2024). Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells. Biomedical Reports, 20, 70. https://doi.org/10.3892/br.2024.1758
MLA
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., Tsuruga, E."Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells". Biomedical Reports 20.4 (2024): 70.
Chicago
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., Tsuruga, E."Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells". Biomedical Reports 20, no. 4 (2024): 70. https://doi.org/10.3892/br.2024.1758
Copy and paste a formatted citation
x
Spandidos Publications style
Sasaki F, Yoshino H, Kusuhara A, Sato K and Tsuruga E: Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells. Biomed Rep 20: 70, 2024.
APA
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., & Tsuruga, E. (2024). Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells. Biomedical Reports, 20, 70. https://doi.org/10.3892/br.2024.1758
MLA
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., Tsuruga, E."Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells". Biomedical Reports 20.4 (2024): 70.
Chicago
Sasaki, F., Yoshino, H., Kusuhara, A., Sato, K., Tsuruga, E."Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells". Biomedical Reports 20, no. 4 (2024): 70. https://doi.org/10.3892/br.2024.1758
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