Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Biomedical Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9434 Online ISSN: 2049-9442
Journal Cover
July-2024 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2024 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Effect of vorinostat on protein expression in vitro and in vivo following mRNA lipoplex administration

  • Authors:
    • Min Tang
    • Yoshiyuki Hattori
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Pharmaceutics, Hoshi University, Shinagawa, Tokyo 142‑8501, Japan
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 105
    |
    Published online on: May 27, 2024
       https://doi.org/10.3892/br.2024.1793
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previously, we demonstrated that cationic liposomes comprised of N‑hexadecyl‑N,N‑dimethylhexadecan‑1-aminium bromide, 1,2‑dioleoyl‑sn‑glycero‑3‑phosphoethanolamine and poly(ethylene glycol) cholesteryl ether induced substantial protein expression both in vitro and in vivo following the administration of mRNA/cationic liposome complexes (mRNA lipoplexes). The present study evaluated the effect of vorinostat, a histone deacetylase inhibitor, on protein expression levels in vitro and in vivo following the administration of mRNA lipoplexes. The half‑maximal inhibitory concentration (IC50) values of vorinostat for human cervical carcinoma HeLa and human liver cancer HepG2 cells were determined to be 7.8 and 2.6 µM, respectively, following a 24 h incubation period. Treatment with 1 µM vorinostat resulted in a 2.7‑fold increase in luciferase (Luc) activity for HeLa cells and a 1.6‑fold increase for HepG2 cells at 24 h post‑transfection with firefly Luc (FLuc) mRNA lipoplexes compared with untreated cells. However, treatment with 10 µM vorinostat decreased Luc activity compared with treatment with 1 µM vorinostat. Intravenous injection of Cy5‑labeled mRNA lipoplexes into mice resulted in mRNA accumulation primarily in the lungs; however, co‑injection with vorinostat at doses of 5 or 25 mg/kg resulted in mRNA accumulation in both the lungs and liver. Furthermore, intravenous injection of FLuc mRNA lipoplexes resulted in high Luc activity in both the lungs and spleen. Nevertheless, co‑injection with vorinostat slightly decreased Luc activity in the lungs but not in the spleen. These findings indicated that vorinostat enhances in vitro protein expression from transfected mRNA after treatment with a lower concentration of IC50; however, it does not largely affect in vivo protein expression from the transfected mRNA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Sahin U, Karikó K and Türeci Ö: mRNA-based therapeutics-developing a new class of drugs. Nat Rev Drug Discov. 13:759–780. 2014.PubMed/NCBI View Article : Google Scholar

2 

Liu T, Liang Y and Huang L: Development and delivery systems of mRNA vaccines. Front Bioeng Biotechnol. 9(718753)2021.PubMed/NCBI View Article : Google Scholar

3 

Ramachandran S, Satapathy SR and Dutta T: Delivery strategies for mRNA vaccines. Pharm Med. 36:11–20. 2022.PubMed/NCBI View Article : Google Scholar

4 

Yen A, Cheng Y, Sylvestre M, Gustafson HH, Puri S and Pun SH: Serum nuclease susceptibility of mRNA cargo in condensed polyplexes. Mol Pharm. 15:2268–2276. 2018.PubMed/NCBI View Article : Google Scholar

5 

Yan Y, Liu XY, Lu A, Wang XY, Jiang LX and Wang JC: Non-viral vectors for RNA delivery. J Control Release. 342:241–279. 2022.PubMed/NCBI View Article : Google Scholar

6 

Hou X, Zaks T, Langer R and Dong Y: Lipid nanoparticles for mRNA delivery. Nat Rev Mater. 6:1078–1094. 2021.PubMed/NCBI View Article : Google Scholar

7 

Buschmann MD, Carrasco MJ, Alishetty S, Paige M, Alameh MG and Weissman D: Nanomaterial delivery systems for mRNA vaccines. Vaccines (Basel). 9(65)2021.PubMed/NCBI View Article : Google Scholar

8 

Tang M, Sagawa A, Inoue N, Torii S, Tomita K and Hattori Y: Efficient mRNA delivery with mRNA lipoplexes prepared using a modified ethanol injection method. Pharmaceutics. 15(1141)2023.PubMed/NCBI View Article : Google Scholar

9 

Gallinari P, Di Marco S, Jones P, Pallaoro M and Steinkühler C: HDACs, histone deacetylation and gene transcription: From molecular biology to cancer therapeutics. Cell Res. 17:195–211. 2007.PubMed/NCBI View Article : Google Scholar

10 

Sakajiri S, Kumagai T, Kawamata N, Saitoh T, Said JW and Koeffler HP: Histone deacetylase inhibitors profoundly decrease proliferation of human lymphoid cancer cell lines. Exp Hematol. 33:53–61. 2005.PubMed/NCBI View Article : Google Scholar

11 

Deng Y, Cheng Q and He J: HDAC inhibitors: Promising agents for leukemia treatment. Biochem Biophys Res Commun. 680:61–72. 2023.PubMed/NCBI View Article : Google Scholar

12 

Duvic M: Histone deacetylase inhibitors for cutaneous T-cell lymphoma. Dermatol Clin. 33:757–764. 2015.PubMed/NCBI View Article : Google Scholar

13 

Ishiguro K and Sartorelli AC: Activation of transiently transfected reporter genes in 3T3 Swiss cells by the inducers of differentiation/apoptosis-dimethylsulfoxide, hexamethylene bisacetamide and trichostatin A. Eur J Biochem. 271:2379–2390. 2004.PubMed/NCBI View Article : Google Scholar

14 

Yamano T, Ura K, Morishita R, Nakajima H, Monden M and Kaneda Y: Amplification of transgene expression in vitro and in vivo using a novel inhibitor of histone deacetylase. Mol Ther. 1:574–580. 2000.PubMed/NCBI View Article : Google Scholar

15 

Hattori Y, Tamaki K, Sakasai S, Ozaki KI and Onishi H: Effects of PEG anchors in pegylated siRNA lipoplexes on in vitro gene-silencing effects and siRNA biodistribution in mice. Mol Med Rep. 22:4183–4196. 2020.PubMed/NCBI View Article : Google Scholar

16 

Guide for the Care and Use of Laboratory Animals: National Research Council (US). 8th Edition. The National Academies Press, Washington, DC, 2011.

17 

Di J, Du Z, Wu K, Jin S, Wang X, Li T and Xu Y: Biodistribution and non-linear gene expression of mRNA LNPs affected by delivery route and particle size. Pharm Res. 39:105–114. 2022.PubMed/NCBI View Article : Google Scholar

18 

Kumagai T, Wakimoto N, Yin D, Gery S, Kawamata N, Takai N, Komatsu N, Chumakov A, Imai Y and Koeffler HP: Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (vorinostat, SAHA) profoundly inhibits the growth of human pancreatic cancer cells. Int J Cancer. 121:656–665. 2007.PubMed/NCBI View Article : Google Scholar

19 

Takai N, Kawamata N, Gui D, Said JW, Miyakawa I and Koeffler HP: Human ovarian carcinoma cells: Histone deacetylase inhibitors exhibit antiproliferative activity and potently induce apoptosis. Cancer. 101:2760–2770. 2004.PubMed/NCBI View Article : Google Scholar

20 

Butler LM, Agus DB, Scher HI, Higgins B, Rose A, Cordon-Cardo C, Thaler HT, Rifkind RA, Marks PA and Richon VM: Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses the growth of prostate cancer cells in vitro and in vivo. Cancer Res. 60:5165–5170. 2000.PubMed/NCBI

21 

Hrzenjak A, Moinfar F, Kremser ML, Strohmeier B, Petru E, Zatloukal K and Denk H: Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo. Mol Cancer. 9(49)2010.PubMed/NCBI View Article : Google Scholar

22 

Wang H, Huang C, Zhao L, Zhang H, Yang JM, Luo P, Zhan BX, Pan Q, Li J and Wang BL: Histone deacetylase inhibitors regulate P-gp expression in colorectal cancer via transcriptional activation and mRNA stabilization. Oncotarget. 7:49848–49858. 2016.PubMed/NCBI View Article : Google Scholar

23 

Eliyahu H, Servel N, Domb AJ and Barenholz Y: Lipoplex-induced hemagglutination: Potential involvement in intravenous gene delivery. Gene Ther. 9:850–858. 2002.PubMed/NCBI View Article : Google Scholar

24 

Simberg D, Weisman S, Talmon Y, Faerman A, Shoshani T and Barenholz Y: The role of organ vascularization and lipoplex-serum initial contact in intravenous murine lipofection. J Biol Chem. 278:39858–39865. 2003.PubMed/NCBI View Article : Google Scholar

25 

Hockly E, Richon VM, Woodman B, Smith DL, Zhou X, Rosa E, Sathasivam K, Ghazi-Noori S, Mahal A, Lowden PA, et al: Suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, ameliorates motor deficits in a mouse model of Huntington's disease. Proc Natl Acad Sci USA. 100:2041–2046. 2003.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tang M and Hattori Y: Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration. Biomed Rep 21: 105, 2024.
APA
Tang, M., & Hattori, Y. (2024). Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration. Biomedical Reports, 21, 105. https://doi.org/10.3892/br.2024.1793
MLA
Tang, M., Hattori, Y."Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration". Biomedical Reports 21.1 (2024): 105.
Chicago
Tang, M., Hattori, Y."Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration". Biomedical Reports 21, no. 1 (2024): 105. https://doi.org/10.3892/br.2024.1793
Copy and paste a formatted citation
x
Spandidos Publications style
Tang M and Hattori Y: Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration. Biomed Rep 21: 105, 2024.
APA
Tang, M., & Hattori, Y. (2024). Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration. Biomedical Reports, 21, 105. https://doi.org/10.3892/br.2024.1793
MLA
Tang, M., Hattori, Y."Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration". Biomedical Reports 21.1 (2024): 105.
Chicago
Tang, M., Hattori, Y."Effect of vorinostat on protein expression <em>in vitro</em> and <em>in vivo</em> following mRNA lipoplex administration". Biomedical Reports 21, no. 1 (2024): 105. https://doi.org/10.3892/br.2024.1793
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team