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Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells

  • Authors:
    • Yen-Ting Wu
    • Cheng-Han Lin
    • Wen-Chin Chiu
    • Tsung-Jen Hsieh
    • Sue-Joan Chang
    • Yun-Ching Chang
    • Yu-Yan Lan
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Golden Hospital, Pingtung 90049, Taiwan, R.O.C., School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan, R.O.C., School of Medicine, College of Medicine, I‑Shou University, Kaohsiung 82445, Taiwan, R.O.C., Department of Life Sciences, National Cheng Kung University, Tainan 70101, Taiwan, R.O.C.
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 143
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    Published online on: August 5, 2024
       https://doi.org/10.3892/br.2024.1831
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Abstract

Although the combination of chemotherapy and radiotherapy has increased the survival rate of patients with nasopharyngeal carcinoma (NPC), certain patients do not respond well to the treatment and have a poor prognosis. Therefore, novel therapeutic drugs and strategies to improve prognosis of patients with NPC are required. As certain plant extracts can suppress the viability of cancer cells, the present study investigated whether oligonol, a polyphenolic compound primarily found in lychee fruit, exerts anticancer activities in NPC cells. MTT, ELISA and immunoblotting were performed to investigate cell survival, cytokeratin‑18 fragment release, and the expression of apoptosis and autophagy markers, respectively. Oligonol decreased the viability of NPC‑TW01 and NPC/HK1NPC cell lines. Oligonol increased the protein expression of several apoptosis markers, including cleaved caspase‑8 and ‑3, cleaved PARP and cytokeratin 18 fragment. Moreover, it also increased expression of autophagy markers Beclin 1 and LC3‑II, as well as LC3‑II/LC3‑I ratio in both NPC cell lines. Furthermore, treatment with autophagy inhibitors 3‑methyladenine or LY294002 significantly increased oligonol‑induced viability inhibition in NPC‑TW01 cells. Combined treatment of oligonol + LY294002 reduced LC3‑II expression and the LC3II/LC3I ratio while increasing cleaved caspase‑8 and ‑3, cleaved PARP and cytokeratin 18 fragment expression in NPC‑TW01 cells. These findings indicated autophagy inhibitors could enhance viability inhibition and apoptotic effects induced by oligonol in NPC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Lin C, Chiu W, Hsieh T, Chang S, Chang Y and Lan Y: Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells. Biomed Rep 21: 143, 2024.
APA
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., & Lan, Y. (2024). Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells. Biomedical Reports, 21, 143. https://doi.org/10.3892/br.2024.1831
MLA
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., Lan, Y."Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells". Biomedical Reports 21.4 (2024): 143.
Chicago
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., Lan, Y."Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells". Biomedical Reports 21, no. 4 (2024): 143. https://doi.org/10.3892/br.2024.1831
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Lin C, Chiu W, Hsieh T, Chang S, Chang Y and Lan Y: Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells. Biomed Rep 21: 143, 2024.
APA
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., & Lan, Y. (2024). Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells. Biomedical Reports, 21, 143. https://doi.org/10.3892/br.2024.1831
MLA
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., Lan, Y."Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells". Biomedical Reports 21.4 (2024): 143.
Chicago
Wu, Y., Lin, C., Chiu, W., Hsieh, T., Chang, S., Chang, Y., Lan, Y."Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells". Biomedical Reports 21, no. 4 (2024): 143. https://doi.org/10.3892/br.2024.1831
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