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Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells

  • Authors:
    • José Luis Gómez‑Olivares
    • Rosa María López‑Durán
    • Sergio Enríquez‑Flores
    • Gabriel López‑Velázquez
    • Ignacio De La Mora‑De La Mora
    • Itzhel García‑Torres
    • Rubí Viedma‑Rodríguez
    • Rafael Valencia‑Quintana
    • Mirta Milić
    • Luis Antonio Flores‑López
  • View Affiliations / Copyright

    Affiliations: Biomembranes Laboratory, Department of Health Sciences, Metropolitan Autonomous University‑Iztapalapa Campus, Mexico City 09310, Mexico, Biomolecules and Infant Health Laboratory, National Institute of Pediatrics, Ministry of Health, Mexico City 04530, Mexico, Morphophysiology Unit, Faculty of Higher Studies‑Iztacala, National Autonomous University of Mexico, Tlalnepantla 54090, Mexico, Rafael Villalobos‑Pietrini Laboratory of Genomic Toxicology and Environmental Chemistry, Faculty of Agrobiology, Autonomous University of Tlaxcala, Tlaxcala 90120, Mexico, Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb 10000, Croatia, Biomolecules and Infant Health Laboratory, CONAHCYT‑National Institute of Pediatrics, Ministry of Health, Mexico City 04530, Mexico
    Copyright: © Gómez‑Olivares et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 158
    |
    Published online on: August 28, 2024
       https://doi.org/10.3892/br.2024.1846
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Abstract

The classic enzymatic function of acetylcholinesterase (AChE) is the hydrolysis of acetylcholine (ACh) in the neuronal synapse. However, AChE is also present in nonneuronal cells such as lymphocytes. Various studies have proposed the participation of AChE in the development of cancer. The ACHE gene produces three mRNAs (T, H and R). AChE‑T encodes amphiphilic monomers, dimers, tetramers (G1A, G2A and G4A) and hydrophilic tetramers (G4H). AChE‑H encodes amphiphilic monomers and dimers (G1A and G2A). AChE‑R encodes a hydrophilic monomer (G1H). The present study considered the differences in the mRNA expression (T, H and R) and protein levels of AChE, as well as the molecular forms of AChE, the glycosylation pattern and the enzymatic activity of AChE present in normal T lymphocytes and leukemic Jurkat E6‑1 cells. The results revealed that AChE enzymatic activity was higher in normal T lymphocytes than in Jurkat cells. Normal T cells expressed AChE‑H transcripts, whereas Jurkat cells expressed AChE‑H and AChE‑T. The molecular forms identified in normal T cells were G2A (5.2 S) and G1A (3.5 S), whereas those in Jurkat cells were G2A (5.2 S), G1A (3.5 S) and G4H (10.6S). AChE in Jurkat cells showed altered posttranslational maturation since a decrease in the incorporation of galactose and sialic acid into its structure was observed. In conclusion, the content and composition of AChE were altered in Jurkat cells compared with those in normal T lymphocytes. The present study opened new avenues for exploring the development of novel therapeutic strategies against T‑cell leukemia and for identifying potential molecular targets for the early detection of this type of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Gómez‑Olivares JL, López‑Durán RM, Enríquez‑Flores S, López‑Velázquez G, De La Mora‑De La Mora I, García‑Torres I, Viedma‑Rodríguez R, Valencia‑Quintana R, Milić M, Flores‑López LA, Flores‑López LA, et al: Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells. Biomed Rep 21: 158, 2024.
APA
Gómez‑Olivares, J.L., López‑Durán, R.M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I. ... Flores‑López, L.A. (2024). Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells. Biomedical Reports, 21, 158. https://doi.org/10.3892/br.2024.1846
MLA
Gómez‑Olivares, J. L., López‑Durán, R. M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I., Viedma‑Rodríguez, R., Valencia‑Quintana, R., Milić, M., Flores‑López, L. A."Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells". Biomedical Reports 21.5 (2024): 158.
Chicago
Gómez‑Olivares, J. L., López‑Durán, R. M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I., Viedma‑Rodríguez, R., Valencia‑Quintana, R., Milić, M., Flores‑López, L. A."Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells". Biomedical Reports 21, no. 5 (2024): 158. https://doi.org/10.3892/br.2024.1846
Copy and paste a formatted citation
x
Spandidos Publications style
Gómez‑Olivares JL, López‑Durán RM, Enríquez‑Flores S, López‑Velázquez G, De La Mora‑De La Mora I, García‑Torres I, Viedma‑Rodríguez R, Valencia‑Quintana R, Milić M, Flores‑López LA, Flores‑López LA, et al: Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells. Biomed Rep 21: 158, 2024.
APA
Gómez‑Olivares, J.L., López‑Durán, R.M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I. ... Flores‑López, L.A. (2024). Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells. Biomedical Reports, 21, 158. https://doi.org/10.3892/br.2024.1846
MLA
Gómez‑Olivares, J. L., López‑Durán, R. M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I., Viedma‑Rodríguez, R., Valencia‑Quintana, R., Milić, M., Flores‑López, L. A."Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells". Biomedical Reports 21.5 (2024): 158.
Chicago
Gómez‑Olivares, J. L., López‑Durán, R. M., Enríquez‑Flores, S., López‑Velázquez, G., De La Mora‑De La Mora, I., García‑Torres, I., Viedma‑Rodríguez, R., Valencia‑Quintana, R., Milić, M., Flores‑López, L. A."Preliminary exploration of the expression of acetylcholinesterase in normal human T lymphocytes and leukemic Jurkat T cells". Biomedical Reports 21, no. 5 (2024): 158. https://doi.org/10.3892/br.2024.1846
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