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Genetic causes of primary immunodeficiency in the Jordanian population

  • Authors:
    • Loiy Obeidat
    • Marwan Abu‑Halaweh
    • Raed Alzyoud
    • Eman Albsoul
    • Apostolos Zaravinos
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    Affiliations: Department of Life Sciences, School of Sciences, European University Cyprus, Nicosia 2404, Cyprus, Department of Biotechnology and Genetics Engineering, Faculty of Science, Philadelphia University, Amman 19392, Jordan, Section of Immunology, Allergy and Rheumatology, Queen Rania Children's Hospital, Queen Rania Al Abdullah Hospital For Children, Amman 11855, Jordan
    Copyright: © Obeidat et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 160
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    Published online on: August 30, 2024
       https://doi.org/10.3892/br.2024.1848
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Abstract

Ιnborn errors of immunity (IEI) represents a heterogenous collection of >480 immune system anomalies, leading to severe infections, autoimmune disorders and malignancies. While these conditions are rare globally, their prevalence is notably higher in the Jordanian population, attributed to elevated rates of consanguinity. The intricate nature of IEI has driven the adoption of genomic technologies for the identification of associated genetic defects. In the present study, whole‑exome sequencing was performed on nine Jordanian IEI patient samples, confirming germline single‑nucleotide variations (SNVs) in 14 genes through Sanger sequencing. Of note, signal transducer and activator of transcription 1 (STAT1), elastase, neutrophil expressed (ELANE) and interferon induced with helicase c domain 1 (IFIH1) harbored mutations that were previously unreported in the Jordanian IEI population. In addition, mutations in capping protein regulator and myosin 1 linker 2 (c.3683C>T), TNFα‑induced protein 3‑interacting protein 1 (TNIP1) (c.460C>G) and STAT1 (c.1061T>C) were confirmed, marking their association with Jordanian IEI. For robustness, the genomic databases Ensemble, Genome AD and ClinVar were used to confirm the SNVs' associations with IEI. Kyoto Encyclopedia of Genes and Genomes pathway analysis also showed involvement of the IL‑17 signaling pathway (including IL‑17 receptor A), T‑helper type 17 cell differentiation (including STAT1), the JAK‑STAT signaling pathway (including STAT2 and tyrosine kinase 2), neutrophil extracellular trap formation (including ELANE), cocaine addiction [G protein signaling modulator 1 (GPSM1)] and cytokine‑cytokine receptor interaction (IL‑17 receptor C). In summary, exome sequencing identified a likely causative genetic defect in ELANE (PID‑28), STAT1 (PID‑30) and IFIH1 (PID‑33). The present findings reveal the association of novel STAT1, ELANE mutations with the clinical phenotype of the patients, as well as known mutations in NLRP12, GPSM1 and TNIP1, in addition to novel ELANE, STAT1 and IFIH1 mutations associated in the context of Jordanian IEI.
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Obeidat L, Abu‑Halaweh M, Alzyoud R, Albsoul E and Zaravinos A: Genetic causes of primary immunodeficiency in the Jordanian population. Biomed Rep 21: 160, 2024.
APA
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., & Zaravinos, A. (2024). Genetic causes of primary immunodeficiency in the Jordanian population. Biomedical Reports, 21, 160. https://doi.org/10.3892/br.2024.1848
MLA
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., Zaravinos, A."Genetic causes of primary immunodeficiency in the Jordanian population". Biomedical Reports 21.5 (2024): 160.
Chicago
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., Zaravinos, A."Genetic causes of primary immunodeficiency in the Jordanian population". Biomedical Reports 21, no. 5 (2024): 160. https://doi.org/10.3892/br.2024.1848
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Spandidos Publications style
Obeidat L, Abu‑Halaweh M, Alzyoud R, Albsoul E and Zaravinos A: Genetic causes of primary immunodeficiency in the Jordanian population. Biomed Rep 21: 160, 2024.
APA
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., & Zaravinos, A. (2024). Genetic causes of primary immunodeficiency in the Jordanian population. Biomedical Reports, 21, 160. https://doi.org/10.3892/br.2024.1848
MLA
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., Zaravinos, A."Genetic causes of primary immunodeficiency in the Jordanian population". Biomedical Reports 21.5 (2024): 160.
Chicago
Obeidat, L., Abu‑Halaweh, M., Alzyoud, R., Albsoul, E., Zaravinos, A."Genetic causes of primary immunodeficiency in the Jordanian population". Biomedical Reports 21, no. 5 (2024): 160. https://doi.org/10.3892/br.2024.1848
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