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Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions

  • Authors:
    • Yuko Nakayama
    • Aya Ino
    • Kazuhiro Yamamoto
    • Kohji Takara
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmaceutics, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, Himeji 670‑8524, Japan, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Hyogo Medical University, Kobe 650‑8530, Japan, Department of Integrated Clinical and Basic Pharmaceutical Sciences, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Kita, Okayama 700‑8558, Japan
    Copyright: © Nakayama et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 184
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    Published online on: October 4, 2024
       https://doi.org/10.3892/br.2024.1872
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Abstract

Everolimus is an oral mammalian target of rapamycin (mTOR) inhibitor used in cancer chemotherapy and transplantation. Due to its therapeutic properties, everolimus has been used long‑term in clinical practice. Drug interactions with everolimus during gastrointestinal absorption can alter the oral bioavailability of everolimus and/or concomitant drugs. However, the effects of everolimus on gastrointestinal absorption remain unknown. The present study assessed the impact of continuous exposure to everolimus on expression and function of the ATP‑binding cassette (ABC) transporter ABCB1 and ABCG2 using a Caco‑2 intestinal cell model. Caco‑2 subline, Caco/EV, was established by continuously exposing Caco‑2 cells to 1 µM everolimus. Cell viability was evaluated using WST‑1 assay. mRNA levels were measured by reverse transcription‑quantitative PCR. Transport activity of ABCB1 was evaluated through the cellular accumulation of Rhodamin 123, a substrate for ABCB1. The half‑maximal inhibitory concentration (IC50) values for everolimus in Caco‑2 and Caco/EV cells were 0.31 and 4.33 µM, respectively, indicating 14‑fold resistance in Caco/EV cells. Sensitivity to paclitaxel and 7‑ethyl‑10‑hydroxycamptothecin, which are substrates for ABCB1 and ABCG2, respectively, was enhanced in Caco/EV, but not in Caco‑2 cells. The IC50 values of cisplatin were comparable in both cell lines. Furthermore, mRNA expression levels of ABCB1 and ABCG2 were lower in Caco/EV cells than in Caco‑2 cells, and the cellular accumulation of Rhodamine 123 was significantly higher in Caco/EV cells. These findings demonstrated that continuous exposure to everolimus suppressed the expression and function of ABCB1 and ABCG2, suggesting potential drug‑drug interactions via the suppression of ABCB1 and ABCG2 in the intestinal tract.
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Copy and paste a formatted citation
Spandidos Publications style
Nakayama Y, Ino A, Yamamoto K and Takara K: Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions. Biomed Rep 21: 184, 2024.
APA
Nakayama, Y., Ino, A., Yamamoto, K., & Takara, K. (2024). Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions. Biomedical Reports, 21, 184. https://doi.org/10.3892/br.2024.1872
MLA
Nakayama, Y., Ino, A., Yamamoto, K., Takara, K."Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions". Biomedical Reports 21.6 (2024): 184.
Chicago
Nakayama, Y., Ino, A., Yamamoto, K., Takara, K."Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions". Biomedical Reports 21, no. 6 (2024): 184. https://doi.org/10.3892/br.2024.1872
Copy and paste a formatted citation
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Spandidos Publications style
Nakayama Y, Ino A, Yamamoto K and Takara K: Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions. Biomed Rep 21: 184, 2024.
APA
Nakayama, Y., Ino, A., Yamamoto, K., & Takara, K. (2024). Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions. Biomedical Reports, 21, 184. https://doi.org/10.3892/br.2024.1872
MLA
Nakayama, Y., Ino, A., Yamamoto, K., Takara, K."Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions". Biomedical Reports 21.6 (2024): 184.
Chicago
Nakayama, Y., Ino, A., Yamamoto, K., Takara, K."Involvement of everolimus‑induced ABCB1 downregulation in drug‑drug interactions". Biomedical Reports 21, no. 6 (2024): 184. https://doi.org/10.3892/br.2024.1872
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