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miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells

  • Authors:
    • Yanrong Guo
    • Xiaoxiao Ding
    • Changling Dai
    • Wenwen Wang
    • Jianlin Chen
    • Sai Chen
    • Linjun Yang
    • Guang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Taizhou Central Hospital (Taizhou University Affiliated Hospital), Taizhou University, Taizhou, Zhejiang 318008, P.R. China, Department of Oncology, Municipal Hospital Affiliated to Medical School of Taizhou University, Taizhou, Zhejiang 318002, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 185
    |
    Published online on: October 4, 2024
       https://doi.org/10.3892/br.2024.1873
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Abstract

Multiple myeloma (MM) is the second most common type of hematological malignancy globally. Despite application of several new drugs, such as daratumumab, bortezomib/lenalidomide/dexamethasone, in combination with hematopoietic stem cell transplantation, overall prognosis remains poor and the pathological mechanism of MM is still unknown. The present study used TargetScan to predict autophagy‑related 7 (ATG7) as a candidate target gene of microRNA (miR)‑1343‑3p and confirmed the interaction between miR‑1343‑3p and the ATG7 3' untranslated region (3'UTR) using a dual‑luciferase reporter assay. In U266 and RPMI‑8226 MM cell lines, miR‑1343‑3p mimic transfection decreased mRNA and protein levels of ATG7, while miR‑1343‑3p inhibition increased ATG7 expression levels using reverse transcription‑qPCR and western blot analysis. miR‑1343‑3p mimic transfection inhibited U266 and RPMI‑8226 cell survival. Finally, miR‑1343‑3p regulated ATG7 and autophagy in MM cells using western blot analysis. The present findings suggested that miR‑1343‑3p may regulate ATG7 and autophagy by directly targeting the 3'UTR of ATG7. To the best of our knowledge, there are no direct data showing the roles of miR‑1343‑3p in development of MM; however, miR‑1343‑3p may be considered a potential target for MM treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Ding X, Dai C, Wang W, Chen J, Chen S, Yang L and Chen G: miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomed Rep 21: 185, 2024.
APA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S. ... Chen, G. (2024). miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomedical Reports, 21, 185. https://doi.org/10.3892/br.2024.1873
MLA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21.6 (2024): 185.
Chicago
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21, no. 6 (2024): 185. https://doi.org/10.3892/br.2024.1873
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Ding X, Dai C, Wang W, Chen J, Chen S, Yang L and Chen G: miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomed Rep 21: 185, 2024.
APA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S. ... Chen, G. (2024). miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomedical Reports, 21, 185. https://doi.org/10.3892/br.2024.1873
MLA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21.6 (2024): 185.
Chicago
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21, no. 6 (2024): 185. https://doi.org/10.3892/br.2024.1873
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