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Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation

  • Authors:
    • Sasikamon Khophai
    • Suwadee Chockchaisiri
    • Krajang Talabnin
    • James R. Ketudat Cairns
    • Chutima Talabnin
  • View Affiliations / Copyright

    Affiliations: School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand, School of Pathology, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
    Copyright: © Khophai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 28
    |
    Published online on: December 6, 2024
       https://doi.org/10.3892/br.2024.1906
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Abstract

Cholangiocarcinoma (CCA) is an aggressive cancer of the bile duct epithelium. Anthocyanins are water‑soluble flavonoids that contribute to the color of fruits and pigmented rice. Black rice bran is rich in anthocyanin pigments and exhibits certain health benefits, including anticancer activity; however, the effect of black rice bran‑derived anthocyanins (BBR‑M‑10) on CCA progression remains unclear. The present study assessed the cytotoxic effects of BBR‑M‑10 using a Sulforhodamine B assay. The metastatic properties of BBR‑M‑10 on CCA cell lines were investigated using wound healing, Transwell in vitro migration and invasion assays. The underlying mechanisms of BBR‑M‑10 bioactivity were examined by quantitative PCR and western blotting. Glycosylation changes were determined by lectin cytochemistry and flow cytometry. The present study demonstrated that BBR‑M‑10 was not toxic to CCA cell lines, but BBR‑M‑10 attenuated CCA cell migration and invasion, as evidenced by the increased expression levels of epithelial markers (F‑actin and claudin‑1), decreased expression levels of mesenchymal markers (vimentin) and a decrease in the activation and phosphorylation of AKT in BBR‑M‑10‑treated CCA cell lines. In addition, aberrant glycosylation was observed in BBR‑M‑10‑treated CCA cell lines, as evidenced by the low expression level of surface Sambucus Nigra lectin‑binding α2,6‑sialylated glycans and the reduction of α2,6 sialyltransferase gene expression levels after BBR‑M‑10 treatment in CCA cell lines. These findings suggested that black rice bran‑derived anthocyanins could potentially be used as anti‑metastatic agents against CCA.
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Copy and paste a formatted citation
Spandidos Publications style
Khophai S, Chockchaisiri S, Talabnin K, Ketudat Cairns JR and Talabnin C: Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation. Biomed Rep 22: 28, 2025.
APA
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J.R., & Talabnin, C. (2025). Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation. Biomedical Reports, 22, 28. https://doi.org/10.3892/br.2024.1906
MLA
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J. R., Talabnin, C."Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation". Biomedical Reports 22.2 (2025): 28.
Chicago
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J. R., Talabnin, C."Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation". Biomedical Reports 22, no. 2 (2025): 28. https://doi.org/10.3892/br.2024.1906
Copy and paste a formatted citation
x
Spandidos Publications style
Khophai S, Chockchaisiri S, Talabnin K, Ketudat Cairns JR and Talabnin C: Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation. Biomed Rep 22: 28, 2025.
APA
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J.R., & Talabnin, C. (2025). Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation. Biomedical Reports, 22, 28. https://doi.org/10.3892/br.2024.1906
MLA
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J. R., Talabnin, C."Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation". Biomedical Reports 22.2 (2025): 28.
Chicago
Khophai, S., Chockchaisiri, S., Talabnin, K., Ketudat Cairns, J. R., Talabnin, C."Black rice bran‑derived anthocyanins attenuate cholangiocarcinoma cell migration via the alteration of epithelial‑mesenchymal transition and sialylation". Biomedical Reports 22, no. 2 (2025): 28. https://doi.org/10.3892/br.2024.1906
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