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Article

Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses

  • Authors:
    • Shih-Yin Chen
    • Ru-Yin Tsai
    • To-Jung Tseng
    • Chin-Chang Chen
  • View Affiliations / Copyright

    Affiliations: School of Chinese Medicine, China Medical University, Taichung 404328, Taiwan, R.O.C., Department of Anatomy, School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C.
  • Article Number: 53
    |
    Published online on: January 27, 2025
       https://doi.org/10.3892/br.2025.1931
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Abstract

Type 2 diabetes mellitus (T2DM) is associated with chronic hyperglycemia, leading to severe complications, including increased risk of cancer. Protein tyrosine phosphatase non‑receptor type 3 (PTPN3) is implicated in both T2DM and cancer progression. The aim of the present study was to investigate the role of PTPN3 genetic polymorphisms and expression in patients with T2DM, as well as to examine changes in body weight, blood glucose levels, and hepatic PTPN3 expression in db/db obese mice in comparison with control mice at 4, 16 and 32 weeks. A total of 469 patients with T2DM and 1,699 healthy control subjects were analyzed for PTPN3 genetic polymorphisms using blood samples. Additionally, the body weight of genetically diabetic obese db/db mice and genotype control mice, and their fasting blood glucose and PTPN3 mRNA and protein expression levels were assessed in the respective liver tissues at different stages of T2DM progression (4, 16 and 32 weeks) using reverse transcription‑quantitative PCR, western blot and immunohistochemistry staining analyses. The allele C frequency of rs75235286 (82.1 vs. 79.1%, P=0.044) and allele G frequency of rs17202063 (82.8% vs. 79.5%, P=0.027) in PTPN3 SNPs differed significantly between T2DM patients and healthy controls. Additionally, the body weight of db/db mice and blood glucose levels were significantly increased from the 4th to 32nd week compared with control mice. Furthermore, db/db mice exhibited significantly elevated hepatic mRNA and protein expression levels of PTPN3 compared with control mice, especially at the 32nd week. Taken together, these findings suggested that an increased level of PTPN3 expression may serve a role in the progression of diabetic complications in patients with T2DM, highlighting the importance of further investigation into PTPN3 as a potential therapeutic target to decrease cancer risk and enhance treatment outcomes in T2DM.
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Copy and paste a formatted citation
Spandidos Publications style
Chen S, Tsai R, Tseng T and Chen C: Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses. Biomed Rep 22: 53, 2025.
APA
Chen, S., Tsai, R., Tseng, T., & Chen, C. (2025). Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses. Biomedical Reports, 22, 53. https://doi.org/10.3892/br.2025.1931
MLA
Chen, S., Tsai, R., Tseng, T., Chen, C."Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses". Biomedical Reports 22.3 (2025): 53.
Chicago
Chen, S., Tsai, R., Tseng, T., Chen, C."Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses". Biomedical Reports 22, no. 3 (2025): 53. https://doi.org/10.3892/br.2025.1931
Copy and paste a formatted citation
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Spandidos Publications style
Chen S, Tsai R, Tseng T and Chen C: Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses. Biomed Rep 22: 53, 2025.
APA
Chen, S., Tsai, R., Tseng, T., & Chen, C. (2025). Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses. Biomedical Reports, 22, 53. https://doi.org/10.3892/br.2025.1931
MLA
Chen, S., Tsai, R., Tseng, T., Chen, C."Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses". Biomedical Reports 22.3 (2025): 53.
Chicago
Chen, S., Tsai, R., Tseng, T., Chen, C."Elevated PTPN3 expression in type 2 diabetes mellitus: Insights from genetic and experimental analyses". Biomedical Reports 22, no. 3 (2025): 53. https://doi.org/10.3892/br.2025.1931
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