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Article Open Access

Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE

  • Authors:
    • Kunwadee Phongpao
    • Wasinee Kheansaard
    • Nuttanan Pholngam
    • Thanaporn Sriwantana
    • Kittiphong Paiboonsukwong
    • Suthat Fucharoen
    • Kovit Pattanapanyasat
    • Nuthawut Sibmooh
    • Pornthip Chaichompoo
    • Saovaros Svasti
  • View Affiliations / Copyright

    Affiliations: Graduate Program in Molecular Medicine, Faculty of Science, Mahidol University, Bangkok 10400, Thailand, Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand, Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan 10540, Thailand, Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand, Center of Excellence for Microparticle and Exosome in Diseases, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    Copyright: © Phongpao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 79
    |
    Published online on: February 28, 2025
       https://doi.org/10.3892/br.2025.1957
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Abstract

Thrombosis is a significant complication in patients with β‑thalassaemia/haemoglobin E (HbE), particularly in splenectomised patients. The endothelium is a key regulator of vascular haemostasis and homeostasis, through the secretion of various regulatory molecules. Nitric oxide (NO), produced by endothelial cells (ECs), regulates vascular functions by acting as a potent vasodilator and an inhibitor of platelet activation. Decreased NO bioavailability, a marker of vascular dysfunction, could be a contributing factor leading to thrombosis. Microparticles or medium extracellular vesicles (mEVs) are associated with thrombosis and vasculopathy in various diseases. Furthermore, elevated levels of mEVs have been observed in splenectomised patients with β‑thalassaemia/HbE and could induce the expression of coagulation proteins, inflammatory cytokines and adhesion molecules in ECs. However, the effects of mEVs on NO regulation by ECs is currently unclear. In the present study, mEVs obtained from splenectomised patients with β‑thalassaemia/HbE had significantly decreased NO production in human pulmonary artery ECs without affecting endothelial nitric oxide synthase expression or phosphorylation. Decreased NO production was attributed to increased haemoglobin levels in mEVs from splenectomised patients, leading to enhanced NO scavenging. These findings highlight a mechanism whereby haemoglobin‑carrying mEVs directly scavenge NO, contributing to vascular dysfunction in β‑thalassaemia/HbE disease.
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Copy and paste a formatted citation
Spandidos Publications style
Phongpao K, Kheansaard W, Pholngam N, Sriwantana T, Paiboonsukwong K, Fucharoen S, Pattanapanyasat K, Sibmooh N, Chaichompoo P, Svasti S, Svasti S, et al: Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE. Biomed Rep 22: 79, 2025.
APA
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S. ... Svasti, S. (2025). Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE. Biomedical Reports, 22, 79. https://doi.org/10.3892/br.2025.1957
MLA
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S., Pattanapanyasat, K., Sibmooh, N., Chaichompoo, P., Svasti, S."Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE". Biomedical Reports 22.5 (2025): 79.
Chicago
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S., Pattanapanyasat, K., Sibmooh, N., Chaichompoo, P., Svasti, S."Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE". Biomedical Reports 22, no. 5 (2025): 79. https://doi.org/10.3892/br.2025.1957
Copy and paste a formatted citation
x
Spandidos Publications style
Phongpao K, Kheansaard W, Pholngam N, Sriwantana T, Paiboonsukwong K, Fucharoen S, Pattanapanyasat K, Sibmooh N, Chaichompoo P, Svasti S, Svasti S, et al: Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE. Biomed Rep 22: 79, 2025.
APA
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S. ... Svasti, S. (2025). Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE. Biomedical Reports, 22, 79. https://doi.org/10.3892/br.2025.1957
MLA
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S., Pattanapanyasat, K., Sibmooh, N., Chaichompoo, P., Svasti, S."Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE". Biomedical Reports 22.5 (2025): 79.
Chicago
Phongpao, K., Kheansaard, W., Pholngam, N., Sriwantana, T., Paiboonsukwong, K., Fucharoen, S., Pattanapanyasat, K., Sibmooh, N., Chaichompoo, P., Svasti, S."Extracellular vesicles modulate endothelial nitric oxide production in patients with β‑thalassaemia/HbE". Biomedical Reports 22, no. 5 (2025): 79. https://doi.org/10.3892/br.2025.1957
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