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Clinical significances of RPL15 gene expression in circulating tumor cells of patients with breast cancer

  • Authors:
    • Ying Zhuang
    • Keli Su
    • Shushu Liu
    • Wei Fan
    • Huijuan Lv
    • Wei Zhong
  • View Affiliations / Copyright

    Affiliations: Breast Cancer Center, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430079, P.R. China, Department of Oncology, The Fourth People's Hospital of Jinan, Jinan, Shandong 250031, P.R. China
    Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 82
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    Published online on: March 11, 2025
       https://doi.org/10.3892/br.2025.1960
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Abstract

The preferred biomarkers for evaluating the outcomes of patients with breast cancer (BC) remain poorly understood. The present study aimed to investigate the predictive roles of circulating tumor cells (CTCs) and ribosomal protein L 15 (RPL15) expression in the prognosis of patients with BC. A total of 170 patients were included in the present study, all of whom were female. BC was diagnosed by combining clinical features, imaging and pathological findings. CanPatrol™ technology and triple color in situ RNA hybridization were used to detect CTC subtypes and RPL15 gene expression levels. CTCs were classified into epithelial CTCs, mesenchymal CTCs (MCTCs), and hybrid CTCs (HCTCs) according to cellular surface markers. Risk factors for recurrence and metastasis were validated by a multivariate COX regression model. Kaplan‑Meier survival curves were used to determine the progression‑free survival (PFS) of patients. The results showed that patients with advanced tumor‑node‑metastasis stage and triple negative BC had high MCTCs, HCTCs and RPL15 levels (P<0.05). Furthermore, the multivariate COX regression analysis revealed that MCTCs, HCTCs, HER2+ and positive RPL15 gene expression were key factors for recurrence and metastasis of patients (P<0.05). The PFS of patients with >2 MCTCs/5 ml blood, >5 HCTCs/5 ml blood, and positive RPL15 gene expression in CTCs were significantly shorter than that of patient with 2 MCTCs, 5 HCTCs, and negative RPL15 gene expression in CTCs (P<0.05). By contrast, the PFS of patients with positive HER2 also was significantly shorter than that of patients with negative HER2. Overall, the present data indicated that the PFS of patients with BC with >2 MCTC or >5 HCTCs, and positive RPL15 gene expression was shorter than that of those with 2 MCTCs or 5 HCTCs, and negative RPL15 gene expression. Additionally, the prognosis of patients with BC with negative HER2 is more favorable than the prognosis of patients with positive HER2 expression.
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Zhuang Y, Su K, Liu S, Fan W, Lv H and Zhong W: Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer. Biomed Rep 22: 82, 2025.
APA
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., & Zhong, W. (2025). Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer. Biomedical Reports, 22, 82. https://doi.org/10.3892/br.2025.1960
MLA
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., Zhong, W."Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer". Biomedical Reports 22.5 (2025): 82.
Chicago
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., Zhong, W."Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer". Biomedical Reports 22, no. 5 (2025): 82. https://doi.org/10.3892/br.2025.1960
Copy and paste a formatted citation
x
Spandidos Publications style
Zhuang Y, Su K, Liu S, Fan W, Lv H and Zhong W: Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer. Biomed Rep 22: 82, 2025.
APA
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., & Zhong, W. (2025). Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer. Biomedical Reports, 22, 82. https://doi.org/10.3892/br.2025.1960
MLA
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., Zhong, W."Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer". Biomedical Reports 22.5 (2025): 82.
Chicago
Zhuang, Y., Su, K., Liu, S., Fan, W., Lv, H., Zhong, W."Clinical significances of <em>RPL15</em> gene expression in circulating tumor cells of patients with breast cancer". Biomedical Reports 22, no. 5 (2025): 82. https://doi.org/10.3892/br.2025.1960
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