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Article Open Access

Effects of aged garlic extract on experimental periodontitis in mice

  • Authors:
    • Canyan Kuang
    • Anna Hirai
    • Chiaki Kamei‑Νagata
    • Hiroshi Nango
    • Masahiro Ohtani
    • Kazuhiro Omori
    • Shogo Takashiba
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Kita-ku, Okayama 700-8525, Japan, Division of Periodontics and Endodontics, Department of Dentistry, Okayama University Hospital, Kita-ku, Okayama 700-8558, Japan, Central Research Institute, Wakunaga Pharmaceutical Co., Ltd., 1Aki-takata, Hiroshima 739-1195, Japan, Department of Pathophysiology-Periodontal Science, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Kita-ku, Okayama 700-8525, Japan
    Copyright: © Kuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 97
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    Published online on: April 11, 2025
       https://doi.org/10.3892/br.2025.1975
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Abstract

Aged garlic extract (AGE) has been reported to exert anti‑inflammatory effects. AGE has been recently found to reduce the inflammatory symptoms of periodontitis, a widespread chronic inflammatory disease caused by oral bacterial infection. However, the mechanisms underlying these effects remain unclear. In the present study, it was aimed to determine the effects of AGE on experimental periodontitis and the related inflammatory factors. AGE (2 g/kg/day) was orally administered to 15 mice during the experimental period, while a control group consisted of 15 mice that received pure water. A total of 3 days after initiation of administration, the left maxillary second molar was ligated with a 5‑0 silk thread for 7 days. Blood biochemical tests were performed to monitor the systemic effects of AGE. Alveolar bone loss was measured morphometrically using a stereomicroscope, and reverse transcription‑quantitative PCR was performed to assay mRNAs of proinflammatory cytokines in gingival tissues. A histological survey was also performed to identify osteoclasts in periodontitis lesions (five mice per group). The total protein and albumin levels showed no significant differences between the AGE and control groups. However, ligation‑induced bone resorption was lower in the AGE group than in the control group (P=0.01). Additionally, ligature increased the mRNA expression of inflammatory cytokines, whereas AGE administration tended to suppress them. Remarkably, tumor necrosis factor gene expression was significantly suppressed (P=0.04). The number of osteoclasts in periodontitis lesions was reduced in the AGE‑treated group. These results indicate that AGE prevents alveolar bone loss by suppressing the inflammatory responses related to osteoclast differentiation in the periodontal tissue. Further research is needed to elucidate the role of AGE in reducing inflammatory bone resorption.
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Copy and paste a formatted citation
Spandidos Publications style
Kuang C, Hirai A, Kamei‑Νagata C, Nango H, Ohtani M, Omori K and Takashiba S: Effects of aged garlic extract on experimental periodontitis in mice. Biomed Rep 22: 97, 2025.
APA
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., & Takashiba, S. (2025). Effects of aged garlic extract on experimental periodontitis in mice. Biomedical Reports, 22, 97. https://doi.org/10.3892/br.2025.1975
MLA
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., Takashiba, S."Effects of aged garlic extract on experimental periodontitis in mice". Biomedical Reports 22.6 (2025): 97.
Chicago
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., Takashiba, S."Effects of aged garlic extract on experimental periodontitis in mice". Biomedical Reports 22, no. 6 (2025): 97. https://doi.org/10.3892/br.2025.1975
Copy and paste a formatted citation
x
Spandidos Publications style
Kuang C, Hirai A, Kamei‑Νagata C, Nango H, Ohtani M, Omori K and Takashiba S: Effects of aged garlic extract on experimental periodontitis in mice. Biomed Rep 22: 97, 2025.
APA
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., & Takashiba, S. (2025). Effects of aged garlic extract on experimental periodontitis in mice. Biomedical Reports, 22, 97. https://doi.org/10.3892/br.2025.1975
MLA
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., Takashiba, S."Effects of aged garlic extract on experimental periodontitis in mice". Biomedical Reports 22.6 (2025): 97.
Chicago
Kuang, C., Hirai, A., Kamei‑Νagata, C., Nango, H., Ohtani, M., Omori, K., Takashiba, S."Effects of aged garlic extract on experimental periodontitis in mice". Biomedical Reports 22, no. 6 (2025): 97. https://doi.org/10.3892/br.2025.1975
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