Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-December 2010 Volume 1 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-December 2010 Volume 1 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings

  • Authors:
    • Koji Arihiro
    • Miyo Oda
    • Katsunari Ogawa
    • Kenshi Tominaga
    • Yoshie Kaneko
    • Tomomi Shimizu
    • Shiho Ohnishi
    • Megumi Oda
    • Yuki Kurita
    • Yuko Taira
    • Masayoshi Fujii
    • Maiko Tanaka
  • View Affiliations / Copyright

    Affiliations: Department of Anatomical Pathology, Hiroshima University Hospital, Hiroshima 734-8551, Japan
  • Pages: 927-932
    |
    Published online on: August 26, 2010
       https://doi.org/10.3892/etm.2010.142
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to compare the results of immunohistochemistry (IHC) assays evaluated by human examiners with the results evaluated by computerized image analysis, and to compare the computerized image analysis results among three automated IHC assays, namely the BioGenex, Dako and Ventana assays. All slides were semiquantitatively evaluated according to the Allred score and J-score by human examiners. The images were analyzed using MacSCOPE version 2.6 for Macintosh according to the H-score and the percentage of positive-stained nuclei per area of carcinoma cells (PP) irrespective of the intensity of the stained nuclei. The H-score for the estrogen receptor (ER) was significantly correlated with the Allred score (P<0.0001) and the PP for the ER was significantly correlated with the J-score (P<0.0001), suggesting that the image analysis used in the present study is a useful method for the evaluation of ER status. Several discrepancies were identified between the Allred score and H-score and between the PP and J-score due to the positive-stained cytoplasm area of carcinoma cells and/or the positive-stained nuclei area of non-carcinoma cells, including benign epithelial cells, lymphocytes and stromal cells. Accordingly, advances in the algorithm of the digitized analyzing system is necessary.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1. 

Goldhirsch A, Ingle JN, Gelber RD, Coates AS, Thurlimann B and Senn HJ: Thresholds for therapies: highlights of the St Gallen International Expert Consensus on the primary therapy of early breast cancer 2009. Ann Oncol. 20:1319–1329. 2009. View Article : Google Scholar : PubMed/NCBI

2. 

Arihiro K, Umemura S, Kurosumi M, et al: Comparison of evaluations for hormone receptors in breast carcinoma using two manual and three automated immunohistochemical assays. Am J Clin Pathol. 127:356–365. 2007. View Article : Google Scholar

3. 

Oda M, Arihiro K, Kataoka T, Osaki A, Asahara T and Ohdan H: Comparison of immunohistochemical assays and reverse transcription real-time polymerase chain reaction for analyzing status of hormone receptors in human breast carcinoma. Pathol Int. 60:305–315. 2010. View Article : Google Scholar

4. 

Bacus S, Flowers JL, Press MF, Bacus JW and McCarty KS Jr: The evaluation of estrogen receptor in primary breast carcinoma by computer-assisted image analysis. Am J Clin Pathol. 90:233–239. 1988.PubMed/NCBI

5. 

Rostagno P, Birtwisle I, Ettore F, et al: Immunohistochemical determination of nuclear antigens by colour image analysis: application for labelling index, estrogen and progesterone receptor status in breast cancer. Anal Cell Pathol. 7:275–287. 1994.

6. 

Layfield LJ, Saria EA, Conlon DH and Kerns BJ: Estrogen and progesterone receptor status determined by the Ventana ES 320 automated immunohistochemical stainer and the CAS 200 image analyzer in 236 early-stage breast carcinomas: prognostic significance. J Surg Oncol. 61:177–184. 1996. View Article : Google Scholar

7. 

Cohen C: Image cytometric analysis in pathology. Hum Pathol. 27:482–493. 1996. View Article : Google Scholar

8. 

Lehr HA, Mankoff DA, Corwin D, Santeusanio G and Gown AM: Application of photoshop-based image analysis to quantification of hormone receptor expression in breast cancer. J Histochem Cytochem. 45:1559–1565. 1997. View Article : Google Scholar : PubMed/NCBI

9. 

Bejar J, Sabo E, Misselevich I, Eldar S and Boss JH: Comparative study of computer-assisted image analysis and light-microscopically determined estrogen receptor status of breast carcinomas. Arch Pathol Lab Med. 122:346–352. 1998.

10. 

Mofidi R, Walsh R, Ridgway PF, et al: Objective measurement of breast cancer oestrogen receptor status through digital image analysis. Eur J Surg Oncol. 29:20–24. 2003. View Article : Google Scholar : PubMed/NCBI

11. 

Rothmann C, Barshack I, Gil A, Goldberg I, Kopolovic J and Malik Z: Potential use of spectral image analysis for the quantitative evaluation of estrogen receptors in breast cancer. Histol Histopathol. 15:1051–1057. 2000.PubMed/NCBI

12. 

Vesoulis Z, Rajappannair L, Define L, Beach J, Schnell B and Myers S: Quantitative image analysis of estrogen receptors in breast fine needle aspiration biopsies. Anal Quant Cytol Histol. 26:323–330. 2004.PubMed/NCBI

13. 

Fisher ER, Anderson S, Dean S, et al: Solving the dilemma of the immunohistochemical and other methods used for scoring estrogen receptor and progesterone receptor in patients with invasive breast carcinoma. Cancer. 103:164–173. 2005. View Article : Google Scholar : PubMed/NCBI

14. 

Gokhale S, Rosen D, Sneige N, et al: Assessment of two automated imaging systems in evaluating estrogen receptor status in breast carcinoma. Appl Immunohistochem Mol Morphol. 15:451–455. 2007. View Article : Google Scholar : PubMed/NCBI

15. 

Hatanaka Y, Hashizume K, Nitta K, Kato T, Itoh I and Tani Y: Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas. Pathol Int. 53:693–699. 2003. View Article : Google Scholar : PubMed/NCBI

16. 

Diaz LK, Sahin A and Sneige N: Interobserver agreement for estrogen receptor immunohistochemical analysis in breast cancer: a comparison of manual and computer-assisted scoring methods. Ann Diagn Pathol. 8:23–27. 2004. View Article : Google Scholar

17. 

Sharangpani GM, Joshi AS, Porter K, et al: Semi-automated imaging system to quantitate estrogen and progesterone receptor immunoreactivity in human breast cancer. J Microsc. 226:244–255. 2007. View Article : Google Scholar : PubMed/NCBI

18. 

Chung GG, Zerkowski MP, Ghosh S, Camp RL and Rimm DL: Quantitative analysis of estrogen receptor heterogeneity in breast cancer. Lab Invest. 87:662–669. 2007. View Article : Google Scholar : PubMed/NCBI

19. 

Turbin DA, Leung S, Cheang MC, et al: Automated quantitative analysis of estrogen receptor expression in breast carcinoma does not differ from expert pathologist scoring: a tissue microarray study of 3,484 cases. Breast Cancer Res Treat. 110:417–426. 2008. View Article : Google Scholar

20. 

Rexhepaj E, Brennan DJ, Holloway P, et al: Novel image analysis approach for quantifying expression of nuclear proteins assessed by immunohistochemistry: application to measurement of oestrogen and progesterone receptor levels in breast cancer. Breast Cancer Res. 10:R892008. View Article : Google Scholar

21. 

Allred DC, Harvey JM, Berardo M and Clark GM: Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol. 11:155–168. 1998.PubMed/NCBI

22. 

Umemura S, Kurosumi M, Moriya T, et al: Immunohistochemical evaluation for hormone receptors in breast cancer: a practically useful evaluation system and handling protocol. Breast Cancer. 13:232–235. 2006. View Article : Google Scholar : PubMed/NCBI

23. 

Goldhirsch A, Glick JH, Gelber RD, Coates AS, Thurlimann B and Senn HJ: Meeting highlights: international expert consensus on the primary therapy of early breast cancer 2005. Ann Oncol. 16:1569–1583. 2005. View Article : Google Scholar : PubMed/NCBI

24. 

Kaplan PA, Frazier SR, Loy TS, Diaz-Arias AA, Bradley K and Bickel JT: 1D5 and 6F11: an immunohistochemical comparison of two monoclonal antibodies for the evaluation of estrogen receptor status in primary breast carcinoma. Am J Clin Pathol. 123:276–280. 2005. View Article : Google Scholar

25. 

Goulding H, Pinder S, Cannon P, et al: A new immunohistochemical antibody for the assessment of estrogen receptor status on routine formalin-fixed tissue samples. Hum Pathol. 26:291–294. 1995. View Article : Google Scholar : PubMed/NCBI

26. 

Shousha S: Oestrogen receptor status of breast carcinoma: Allred/H score conversion table. Histopathology. 53:346–347. 2008. View Article : Google Scholar : PubMed/NCBI

27. 

Sapino A, Cassoni P, Ferrero E, et al: Estrogen receptor alpha is a novel marker expressed by follicular dendritic cells in lymph nodes and tumor-associated lymphoid infiltrates. Am J Pathol. 163:1313–1320. 2003. View Article : Google Scholar

28. 

Kim R, Kaneko M, Arihiro K, et al: Extranuclear expression of hormone receptors in primary breast cancer. Ann Oncol. 17:1213–1220. 2006. View Article : Google Scholar : PubMed/NCBI

29. 

Kostopoulos S, Glotsos D, Cavouras D, et al: Computer-based association of the texture of expressed estrogen receptor nuclei with histologic grade using immunohistochemically-stained breast carcinomas. Anal Quant Cytol Histol. 31:187–196. 2009.

30. 

Taylor CR and Levenson RM: Quantification of immunohistochemistry – issues concerning methods, utility and semiquantitative assessment II. Histopathology. 49:411–424. 2006.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Arihiro K, Oda M, Ogawa K, Tominaga K, Kaneko Y, Shimizu T, Ohnishi S, Oda M, Kurita Y, Taira Y, Taira Y, et al: Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings. Exp Ther Med 1: 927-932, 2010.
APA
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T. ... Tanaka, M. (2010). Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings. Experimental and Therapeutic Medicine, 1, 927-932. https://doi.org/10.3892/etm.2010.142
MLA
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T., Ohnishi, S., Oda, M., Kurita, Y., Taira, Y., Fujii, M., Tanaka, M."Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings". Experimental and Therapeutic Medicine 1.6 (2010): 927-932.
Chicago
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T., Ohnishi, S., Oda, M., Kurita, Y., Taira, Y., Fujii, M., Tanaka, M."Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings". Experimental and Therapeutic Medicine 1, no. 6 (2010): 927-932. https://doi.org/10.3892/etm.2010.142
Copy and paste a formatted citation
x
Spandidos Publications style
Arihiro K, Oda M, Ogawa K, Tominaga K, Kaneko Y, Shimizu T, Ohnishi S, Oda M, Kurita Y, Taira Y, Taira Y, et al: Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings. Exp Ther Med 1: 927-932, 2010.
APA
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T. ... Tanaka, M. (2010). Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings. Experimental and Therapeutic Medicine, 1, 927-932. https://doi.org/10.3892/etm.2010.142
MLA
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T., Ohnishi, S., Oda, M., Kurita, Y., Taira, Y., Fujii, M., Tanaka, M."Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings". Experimental and Therapeutic Medicine 1.6 (2010): 927-932.
Chicago
Arihiro, K., Oda, M., Ogawa, K., Tominaga, K., Kaneko, Y., Shimizu, T., Ohnishi, S., Oda, M., Kurita, Y., Taira, Y., Fujii, M., Tanaka, M."Comparison of evaluations of hormone receptors in breast carcinoma by image-analysis using three automated immunohistochemical stainings". Experimental and Therapeutic Medicine 1, no. 6 (2010): 927-932. https://doi.org/10.3892/etm.2010.142
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team