Activin A maintains pluripotency markers and proliferative potential of human induced pluripotent stem cells

Tomizawa,Minoru; Shinozaki,Fuminobu; Sugiyama,Takao; Yamamoto,Shigenori; Sueishi,Makoto; Yoshida,Takanobu

doi:10.3892/etm.2011.219

May-June 2011 Volume 2 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-June 2011 Volume 2 Issue 3

Full Size Image

Article

Activin A maintains pluripotency markers and proliferative potential of human induced pluripotent stem cells

Authors:
- Minoru Tomizawa
- Fuminobu Shinozaki
- Takao Sugiyama
- Shigenori Yamamoto
- Makoto Sueishi
- Takanobu Yoshida
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan, Department of Radiology, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan, Department of Rheumatology, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan, Department of Pediatrics, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan, Department of Internal Medicine, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
Pages: 405-408
|
Published online on: February 28, 2011

https://doi.org/10.3892/etm.2011.219
Expand metrics +

Abstract

To investigate the role of Activin A in the embryoid bodies (EBs) of human induced pluripotent stem (iPS) cells, EBs were transferred onto dishes coated with Matrigel after 4 days of incubation with Activin A and observed under a microscope. Alkaline phosphatase staining and immunostaining were performed to analyze the pluripotency of the cells, and the MTS assay was performed to analyze their proliferative potential. Fourteen days after EB formation, cells cultured with Activin A (100 ng/ml) showed no morphological alterations. Cells cultured with 10-100 ng/ml of Activin A were positive for alkaline phosphatase staining, while cells cultured with 0-3 ng/ml showed negative staining. Cells cultured with 10 ng/ml of Activin A were positive for Oct3/4, Nanog, SSEA-4 and TRA-1-60, while cells cultured with 0 ng/ml Activin A were negative. Cells cultured with 3-30 ng/ml of Activin A maintained their proliferative potential, while loss of proliferative potential was observed in cells cultured with 100 ng/ml Activin A. In conclusion, Activin A maintained pluripotency markers in human iPS cells cultured as EBs with Activin A.

View Figures

View References

1.	Sulzbacher S, Schroeder IS, Truong TT and Wobus AM: Activin A-induced differentiation of embryonic stem cells into endoderm and pancreatic progenitors – the influence of differentiation factors and culture conditions. Stem Cell Rev. 5:159–173. 2009.
2.	Smith JC, Price BM, van Nimmen K and Huylebroeck D: Identification of a potent Xenopus mesoderm-inducing factor as a homologue of Activin A. Nature. 345:729–731. 1990. View Article : Google Scholar : PubMed/NCBI
3.	Thomsen G, Woolf T, Whitman M, et al: Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures. Cell. 63:485–493. 1990. View Article : Google Scholar : PubMed/NCBI
4.	D’Amour KA, Agulnick AD, Eliazer S, Kelly OG, Kroon E and Baetge EE: Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat Biotechnol. 23:1534–1541. 2005.
5.	Yao S, Chen S, Clark J, et al: Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci USA. 103:6907–6912. 2006. View Article : Google Scholar : PubMed/NCBI
6.	Xiao L, Yuan X and Sharkis SJ: Activin A maintains self-renewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells. Stem Cells. 24:1476–1486. 2006. View Article : Google Scholar : PubMed/NCBI
7.	Touboul T, Hannan NR, Corbineau S, et al: Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology. 51:1754–1765. 2010. View Article : Google Scholar : PubMed/NCBI
8.	Tomizawa M, Toyama Y, Ito C, et al: Hepatoblast-like cells enriched from mouse embryonic stem cells in medium without glucose, pyruvate, arginine, and tyrosine. Cell Tissue Res. 333:17–27. 2008. View Article : Google Scholar : PubMed/NCBI
9.	Basma H, Soto-Gutierrez A, Yannam GR, et al: Differentiation and transplantation of human embryonic stem cell-derived hepatocytes. Gastroenterology. 136:990–999. 2009. View Article : Google Scholar : PubMed/NCBI
10.	Vallier L, Touboul T, Brown S, et al: Signaling pathways controlling pluripotency and early cell fate decisions of human induced pluripotent stem cells. Stem Cells. 27:2655–2666. 2009. View Article : Google Scholar : PubMed/NCBI
11.	Beattie GM, Lopez AD, Bucay N, et al: Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells. 23:489–495. 2005. View Article : Google Scholar : PubMed/NCBI
12.	Sato N, Meijer L, Skaltsounis L, Greengard P and Hemmati-Brivanlou A: Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med. 10:55–63. 2004. View Article : Google Scholar : PubMed/NCBI
13.	James D, Levine AJ, Besser D and Hemmati-Brivanlou A: TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells. Development. 132:1273–1282. 2005. View Article : Google Scholar : PubMed/NCBI
14.	Vallier L, Mendjan S, Brown S, et al: Activin/Nodal signalling maintains pluripotency by controlling Nanog expression. Development. 136:1339–1349. 2009. View Article : Google Scholar : PubMed/NCBI
15.	Vallier L, Alexander M and Pedersen RA: Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci. 118:4495–4509. 2005. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Activin A maintains pluripotency markers and proliferative potential of human induced pluripotent stem cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles