Functional interaction of BRCA1/ATM-associated BAAT1 with the DNA-PK catalytic subunit

So,Eui Young; Ouchi,Toru

doi:10.3892/etm.2011.232

May-June 2011 Volume 2 Issue 3

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May-June 2011 Volume 2 Issue 3

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Functional interaction of BRCA1/ATM-associated BAAT1 with the DNA-PK catalytic subunit

Authors:
- Eui Young So
- Toru Ouchi
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Affiliations: Department of Medicine, NUHS, Systems Biology Program, Pritzker School of Medicine, The University of Chicago, Evanston, IL 60201, USA
Pages: 443-447
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Published online on: March 21, 2011

https://doi.org/10.3892/etm.2011.232
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Abstract

Ataxia telangiectasia mutated (ATM) and DNA-dependent protein kinase (DNA-PK) play a crucial role in the initial stages of cell response, when cells are exposed to DNA insult such as ionizing radiation (IR) and chemical agents. We previously demonstrated that ATM requires BAAT1 for its activation in response to IR. In the present study, BAAT1 was found to bind to the DNA-PK catalytic subunit (DNA-PKcs) and SMC1. Biochemical analysis indicated that several regions of BAAT1 were responsible for the interaction with these proteins, and their binding affinity was altered after treatment with the IR mimetic, neocarzinostatin (NCS). Phosphorylation of the DNA-PKcs at Ser2056 and SMC1 at Ser966 was induced by NCS, while phosphorylation was reduced when BAAT1 was depleted by siRNA. These results indicate that BAAT1 globally regulates DNA damage signaling during the early stages of apoptosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Functional interaction of BRCA1/ATM-associated BAAT1 with the DNA-PK catalytic subunit

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