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Article

Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro

  • Authors:
    • Jun Yu
    • Yisheng Zhong
    • Yu Cheng
    • Xi Shen
    • Jun Wang
    • Yiyong Wei
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China
  • Pages: 513-516
    |
    Published online on: March 21, 2011
       https://doi.org/10.3892/etm.2011.239
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Abstract

The aim of the present study was to examine the expression of glutamine synthetase (GS) in rat retinal Müller cells induced by different levels of hydrostatic pressure using a novel pressure mechanism. pH, PCO2 or PO2 in culture medium as determined by gas analysis was used to examine the pressure mechanism. GS expression in the Müller cells at different levels of hydrostatic pressure (0, 20, 40, 60 and 80 mmHg/24 h) was examined using immunofluorescence, real-time PCR and Western blotting. There was no significant difference in pH, PCO2 or PO2 in the culture medium by gas analysis at the different hydrostatic pressure levels (p>0.05). Immunofluorescence staining showed that GS was expressed in the Müller cells. The expression of GS in the 40 mmHg/24 h and the 60 mmHg/24 h groups was increased significantly compared to that in the 0 mmHg/24 h group (p<0.05). These results suggest that the pressure mechanism which was constructed was effective and that moderate pressure promotes the up-regulation of GS in active Müller cells.
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1. 

John C and Morrison MD: Integrins in the optic nerve head: potential roles in glaucomatous optic neuropathy. Trans Am Ophthalmol Soc. 104:453–477. 2006.PubMed/NCBI

2. 

Bull ND, Limb GA and Martin KR: Human Müller stem cell (MIO-M1) transplantation in a rat model of glaucoma: survival, differentiation and integration. Invest Ophthalmol Vis Sci. 49:3449–3456. 2008.

3. 

Quigley HA: New paradigms in the mechanisms and management of glaucoma. Eye. 19:1241–1248. 2005. View Article : Google Scholar : PubMed/NCBI

4. 

Sommer A: Intraocular pressure and glaucoma. Am J Ophthalmol. 107:186–188. 1989. View Article : Google Scholar

5. 

Carter-Dawson L, Shen F, Harwerth RS, Smith EL, Crawford ML and Chang A: Glutamine immunoreactivity in Müller cells of monkey eyes with experimental glaucoma. Exp Eye Res. 66:537–545. 1998.

6. 

Massey SC: Cell types using glutamate as a neurotransmitter in the vertebrate retina. Progress in Retinal Research. Osborne NN and Chader CJ: Pergamon; Oxford: pp. 399–425. 1990, View Article : Google Scholar

7. 

Pow DV and Robinson SR: Glutamate in some retinal neurons is derived solely from glia. Neuroscience. 60:355–366. 1994. View Article : Google Scholar : PubMed/NCBI

8. 

Ambati J, Chalam KV and Chawla DK: Elevated gammaaminobutyric acid, glutamate, and vascular endothelial growth factor levels in the vitreous of patients with proliferative diabetic retinopathy. Arch Ophthalmol. 115:1161–1166. 1997. View Article : Google Scholar

9. 

Shen F, Chen B, Danias J, Lee KC, Lee H and Su Y: Glutamate-induced glutamine synthetase expression in retinal Müller cells after short-term ocular hypertension in the rat. Invest Ophthalmol Vis Sci. 45:3107–3112. 2004.

10. 

Riepe RE and Norenberg MD: Müller cell localization of glutamine synthetase in the rat retina. Nature. 268:654–655. 1977.

11. 

Rauen T and Wiebner M: Fine tuning of glutamate uptake and degradation in glial cells: common transcriptional regulation of GLAST1 and GS. Neurochem Int. 37:179–189. 2000. View Article : Google Scholar : PubMed/NCBI

12. 

Linser PJ, Sorrentino M and Moscona AA: Cellular compartmentalization of carbonic anhydrase-C and glutamine synthethase in developing and mature mouse neural retina. Brain Res. 315:65–71. 1984. View Article : Google Scholar : PubMed/NCBI

13. 

Newman E and Reichenbach A: The Müller cells: a functional element of the retina. Trends Neurosci. 19:307–312. 1996.

14. 

Dreyer EB, Zurakowski D, Schumer RA, Podos SM and Lipton SA: Elevated glutamate in vitreous body of humans and monkeys with glaucoma. Arch Opthalmol. 114:299–305. 1996. View Article : Google Scholar : PubMed/NCBI

15. 

Brooks DE, Garcia GA, Dreyer EB, Zurakowski D and Franco-Bourland RE: Vitreous body glutamate concentration in dogs with glaucoma. Am J Vet Res. 58:864–867. 1997.PubMed/NCBI

16. 

Dkhissi O, Chanut E and Wasowicz M: Retinal TUNEL-positive cells and high glutamate levels in vitreous humor of mutant quail with a glaucoma-like disorder. Invest Ophthalmol Vis Sci. 40:990–995. 1999.PubMed/NCBI

17. 

Carter-Dawson L, Shen F, Harwerth RS, Smith EL, Crawford ML and Chuang A: Glutamine immunoreactivity in Müller cells of monkey eyes with experimental glaucoma. Exp Eye Res. 66:537–545. 1998.

18. 

Li S, Wang J, Wang D and Bai H: The experimental study of effect of pressure on rat Müller cells in vitro. Chin J Ophthamol. 41:325–329. 2005.

19. 

Woldemussie E, Wijono M and Ruiz G: Müller cell response to laser-induced increase in intraocular pressure in rats. Glia. 47:109–119. 2004.

20. 

Dreyer EB, Zurakowski D, Schumer RA, Podos SM and Lipton SA: Elevated glutamate in vitreous body of humans and monkeys with glaucoma. Arch Opthalmol. 114:299–305. 1996. View Article : Google Scholar : PubMed/NCBI

21. 

Carter-Dawson L, Crauford ML and Harwerth RS: Vitreal glutamate concentration in monkeys with experimental glaucoma. Invest Opthalmol Vis Sci. 43:2633–2637. 2002.PubMed/NCBI

22. 

Honkanen RA, Baruah S and Zimmerman MB: Vitreous amino acid levels in patients with glaucoma undergoing vitrectomy. Arch Ophthalmol. 121:183–188. 2003. View Article : Google Scholar : PubMed/NCBI

23. 

Johnson TV and Tomarev SI: Rodent models of glaucoma. Brain Res Bull. 81:349–358. 2010. View Article : Google Scholar

24. 

Agar A, Yip SS, Hill MA and Coroneo MT: Pressure-related apoptosis in neuronal cell lines. J Neurosci Res. 60:495–503. 2000. View Article : Google Scholar : PubMed/NCBI

25. 

Sappington RM, Chan M and Calkins DJ: Interleukin-6 protects retinal ganglion cells from pressure-induced death. Invest Opthalmol Vis Sci. 47:2932–2942. 2006. View Article : Google Scholar : PubMed/NCBI

26. 

Salvador-Silva M, Ricard CS, Agapova OA, Yang P and Hernandez MR: Expression of small heat shock proteins and intermediate filaments in the human optic nerve head astrocytes exposed to elevated hydrostatic pressure in vitro. J Neurosci Res. 66:59–73. 2001. View Article : Google Scholar

27. 

Tezel G and Wax MB: Hypoxia-inducible factor 1α in the glaucomatous retina and optic nerve head. Arch Ophthalmol. 122:1348–1356. 2004.

28. 

Reichenbach A, Wurm A, Pannicke T, Landiev I, Wiedemann P and Bringmann A: Müller cells as players in retinal degeneration and edema. Graefe’s Arch Clin Exp Ophthalmol. 245:627–636. 2007.

29. 

Walsh N, Valter K and Stone J: Cellular and subcellular patterns of expression of bFGF and CNTF in the normal and light stressed adult rat retina. Curr Eye Res. 72:495–501. 2001. View Article : Google Scholar : PubMed/NCBI

30. 

Harada T, Harada C and Kohsaka S: Microglia-Müller glia cell interactions control neurotrophic factor production during lightinduced retinal degeneration. Neurosei. 22:9228–9236. 2002.

31. 

Pow DV and Crook DK: Direct immunocytochemical evidence for the transfer of glutamine from glial cells to neurons: use of specific antibodies directed against the D-steroisomers of glutamate and glutamine. Neuroscience. 70:295–302. 1996. View Article : Google Scholar

32. 

Johnson TV and Tomarev SI: Rodent models of glaucoma. Brain Research Bulletin. 81:349–358. 2010. View Article : Google Scholar

33. 

Tsacopoulos M: Metabolic signaling between neurons and glial cells: a short review. J Physiol Paris. 96:283–288. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Zhong Y, Cheng Y, Shen X, Wang J and Wei Y: Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro. Exp Ther Med 2: 513-516, 2011.
APA
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., & Wei, Y. (2011). Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro. Experimental and Therapeutic Medicine, 2, 513-516. https://doi.org/10.3892/etm.2011.239
MLA
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., Wei, Y."Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro". Experimental and Therapeutic Medicine 2.3 (2011): 513-516.
Chicago
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., Wei, Y."Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro". Experimental and Therapeutic Medicine 2, no. 3 (2011): 513-516. https://doi.org/10.3892/etm.2011.239
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Zhong Y, Cheng Y, Shen X, Wang J and Wei Y: Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro. Exp Ther Med 2: 513-516, 2011.
APA
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., & Wei, Y. (2011). Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro. Experimental and Therapeutic Medicine, 2, 513-516. https://doi.org/10.3892/etm.2011.239
MLA
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., Wei, Y."Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro". Experimental and Therapeutic Medicine 2.3 (2011): 513-516.
Chicago
Yu, J., Zhong, Y., Cheng, Y., Shen, X., Wang, J., Wei, Y."Effect of high hydrostatic pressure on the expression of glutamine synthetase in rat retinal Müller cells cultured in vitro". Experimental and Therapeutic Medicine 2, no. 3 (2011): 513-516. https://doi.org/10.3892/etm.2011.239
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