Identification of novel molecular markers for detection of gastric cancer cells in the peripheral blood circulation using genome-wide microarray analysis

  • Authors:
    • Nobuyuki Matsumura
    • Hitoshi Zembutsu
    • Koji Yamaguchi
    • Kazuaki Sasaki
    • Tetsuhiro Tsuruma
    • Toshihiko Nishidate
    • Ryuichi Denno
    • Koichi Hirata
  • View Affiliations

  • Published online on: April 8, 2011     https://doi.org/10.3892/etm.2011.252
  • Pages: 705-713
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Abstract

Although metastasis or relapse is a leading cause of death for patients with gastric cancer, the hematogenous spread of cancer cells remains undetected at the time of initial therapy. The development of novel diagnostic molecular marker(s) to detect circulating gastric cancer cells is an issue of great clinical importance. We obtained peripheral blood samples from 10 patients with gastric cancer who underwent laparotomy and 4 healthy volunteers. Microarray analysis consisting of 30,000 genes or ESTs was carried out using eight gastric cancer tissues and normal gastric mucosae. We selected 53 genes up-regulated in gastric cancer compared to normal gastric mucosae from our microarray data set, and, among these, identified five candidate marker genes (TSPAN8, EPCAM, MMP12, MMP7 and REG3A) which were not expressed in peripheral blood mononuclear cells (PBMCs) from 4 healthy volunteers. We further carried out semi-quantitative nested reverse transcription-polymerase chain reaction (RT-PCR) for HRH1, EGFR, CK20 and CEA in addition to the five newly identified genes using PBMCs of patients with gastric cancer, and found that expression of one or more genes out of the nine was detected in 80% of the patients with gastric cancer. Moreover, the numbers of genes expressed in PBMCs were ≤2 and ≥2 in all vascular invasion-negative cases and in 5 of 6 positive cases, respectively, showing significant differences between the two groups (P=0.041). Nested RT-PCR analysis for the set of nine marker genes using PBMCs may provide the potential for detection of circulating gastric cancer cells prior to metastasis formation in other organs.
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July-August 2011
Volume 2 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Matsumura N, Zembutsu H, Yamaguchi K, Sasaki K, Tsuruma T, Nishidate T, Denno R and Hirata K: Identification of novel molecular markers for detection of gastric cancer cells in the peripheral blood circulation using genome-wide microarray analysis. Exp Ther Med 2: 705-713, 2011
APA
Matsumura, N., Zembutsu, H., Yamaguchi, K., Sasaki, K., Tsuruma, T., Nishidate, T. ... Hirata, K. (2011). Identification of novel molecular markers for detection of gastric cancer cells in the peripheral blood circulation using genome-wide microarray analysis. Experimental and Therapeutic Medicine, 2, 705-713. https://doi.org/10.3892/etm.2011.252
MLA
Matsumura, N., Zembutsu, H., Yamaguchi, K., Sasaki, K., Tsuruma, T., Nishidate, T., Denno, R., Hirata, K."Identification of novel molecular markers for detection of gastric cancer cells in the peripheral blood circulation using genome-wide microarray analysis". Experimental and Therapeutic Medicine 2.4 (2011): 705-713.
Chicago
Matsumura, N., Zembutsu, H., Yamaguchi, K., Sasaki, K., Tsuruma, T., Nishidate, T., Denno, R., Hirata, K."Identification of novel molecular markers for detection of gastric cancer cells in the peripheral blood circulation using genome-wide microarray analysis". Experimental and Therapeutic Medicine 2, no. 4 (2011): 705-713. https://doi.org/10.3892/etm.2011.252