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Review

Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review)

  • Authors:
    • Hui Wang
    • Hai-Bo Shi
    • Shan-Kai Yin
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Affiliated Sixth People's Hospital of Shanghai Jiao Tong University, Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 777-781
    |
    Published online on: June 27, 2011
       https://doi.org/10.3892/etm.2011.296
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Abstract

Hair cells in the cochlea can be damaged by various insults, including noise, drugs, infections and presbycusis, which may cause sensorineural hearing loss. Gene therapy is a novel therapeutic technology that, recently, has led to the idea of treating inner ear diseases on a genetic level. Depending on their characteristics, such as a high efficiency in delivery, the capability of specific targeting, multifunctionality, biodegradability, non-toxicity, non-immunogenicity and the capability of limiting DNA degradation, nanovectors, such as polyamidoamine (PAMAM) dendrimers for cellular gene delivery, provide a promising approach to eradicate genetic diseases. They are a new class of highly branched spherical polymers that are highly soluble in aqueous solution. Their unique surface is composed of positively charged primary amine groups which allow them to form stable complexes with plasmid DNA, oligonucleotides, antibodies and drugs. This review provides an overview of the characteristics of PAMAMs which may be used in gene transfer into the cochlea as well as the efforts to improve their transfection efficiency as gene-delivery carriers.
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1. 

Duan M, Venail F, Spencer N and Mezzina M: Treatment of peripheral sensorineural hearing loss: gene therapy. Gene Ther. 11(Suppl 1): 51–56. 2004. View Article : Google Scholar : PubMed/NCBI

2. 

Ge X, Jackson RL, Liu J, et al: Distribution of PLGA nanoparticles in chinchilla cochleae. Otolaryngol Head Neck Surg. 137:619–623. 2007. View Article : Google Scholar : PubMed/NCBI

3. 

Tamura T, Kita T, Nakagawa T, et al: Drug delivery to the cochlea using PLGA nanoparticles. Laryngoscope. 115:2000–2005. 2005. View Article : Google Scholar : PubMed/NCBI

4. 

Zou J, Saulnier P, Perrier T, et al: Distribution of lipid nanocapsules in different cochlear cell populations after round window membrane permeation. J Biomed Mater Res B Appl Biomater. 87:10–18. 2008. View Article : Google Scholar : PubMed/NCBI

5. 

Praetorius M, Brunner C, Lehnert B, et al: Transsynaptic delivery of nanoparticles to the central auditory nervous system. Acta Otolaryngol. 127:486–490. 2007. View Article : Google Scholar : PubMed/NCBI

6. 

Sun H, Jiang M and Zhu SH: In vitro and in vivo studies on hydroxyapatite nanoparticles as a novel vector for inner ear gene therapy. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 43:51–57. 2008.PubMed/NCBI

7. 

Jiang M, Zhang YQ, He GX and Sun H: Protective effect of NT-3 gene mediated by hydroxyapatite nanoparticle on the cochlea of guinea pigs injured by excitotoxicity. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 32:563–567. 2007.PubMed/NCBI

8. 

Roy S, Johnston AH, Newman TA, et al: Cell-specific targeting in the mouse inner ear using nanoparticles conjugated with a neurotrophin-derived peptide ligand: potential tool for drug delivery. Int J Pharm. 390:214–224. 2010. View Article : Google Scholar : PubMed/NCBI

9. 

Praetorius M, Pfannenstiel S, Klingmann C, Baumann I, Plinkert PK and Staecker H: [Expression patterns of non-viral transfection with GFP in the organ of Corti in vitro and in vivo. Gene therapy of the inner ear with non-viral vectors]. HNO. 56:524–529. 2008.(In German).

10. 

Zhang Y, Liu JY, Yang F, et al: A new strategy for assembling multifunctional nanocomposites with iron oxide and amino-terminated PAMAM dendrimers. J Mater Sci Mater Med. 20:2433–2440. 2009. View Article : Google Scholar : PubMed/NCBI

11. 

Liu XX, Rocchi P, Qu FQ, et al: PAMAM dendrimers mediate siRNA delivery to target Hsp27 and produce potent antiproliferative effects on prostate cancer cells. ChemMedChem. 4:1302–1310. 2009. View Article : Google Scholar

12. 

Navarro G and de Ilarduya CT: Activated and non-activated PAMAM dendrimers for gene delivery in vitro and in vivo. Nanomedicine. 5:287–297. 2009. View Article : Google Scholar : PubMed/NCBI

13. 

Tomalia DA, Baker H, Dewald J, Hall M, Kallos G, Martin S, Roeck J, Ryder J and Smith P: A new class of polymers: starburst-dendritic macromolecules. Polymer J. 17:1171985. View Article : Google Scholar

14. 

Tang MX, Redemann CT and Szoka FC Jr: In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjug Chem. 7:703–714. 1996. View Article : Google Scholar : PubMed/NCBI

15. 

Jansen JF, de Brabander-van den Berg EM and Meijer EW: Encapsulation of guest molecules into a dendritic box. Science. 266:1226–1229. 1994. View Article : Google Scholar : PubMed/NCBI

16. 

Roberts JC, Bhalgat MK and Zera RT: Preliminary biological evaluation of polyamidoamine (PAMAM) Starburst dendrimers. J Biomed Mater Res. 30:53–65. 1996. View Article : Google Scholar : PubMed/NCBI

17. 

Nam HY, Hahn HJ, Nam K, et al: Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery. Int J Pharm. 363:199–205. 2008. View Article : Google Scholar : PubMed/NCBI

18. 

Lee H and Larson RG: Multiscale modeling of dendrimers and their interactions with bilayers and polyelectrolytes. Molecules. 14:423–438. 2009. View Article : Google Scholar : PubMed/NCBI

19. 

Landmark KJ, Dimaggio S, Ward J, et al: Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers. ACS Nano. 2:773–783. 2008. View Article : Google Scholar : PubMed/NCBI

20. 

Tekade RK, Kumar PV and Jain NK: Dendrimers in oncology: an expanding horizon. Chem Rev. 109:49–87. 2009. View Article : Google Scholar : PubMed/NCBI

21. 

Klajnert B and Bryszewska M: Dendrimers: properties and applications. Acta Biochim Pol. 48:199–208. 2001.PubMed/NCBI

22. 

Shakhbazau A, Isayenka I, Kartel N, et al: Transfection efficiencies of PAMAM dendrimers correlate inversely with their hydrophobicity. Int J Pharm. 383:228–235. 2010. View Article : Google Scholar : PubMed/NCBI

23. 

Haensler J and Szoka FC Jr: Polyamidoamine cascade polymers mediate efficient transfection of cells in culture. Bioconjug Chem. 4:372–379. 1993. View Article : Google Scholar : PubMed/NCBI

24. 

Kukowska-Latallo JF, Bielinska AU, Johnson J, Spindler R, Tomalia DA and Baker JR Jr: Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers. Proc Natl Acad Sci USA. 93:4897–4902. 1996. View Article : Google Scholar : PubMed/NCBI

25. 

Bielinska AU, Kukowska-Latallo JF and Baker JR Jr: The interaction of plasmid DNA with polyamidoamine dendrimers: mechanism of complex formation and analysis of alterations induced in nuclease sensitivity and transcriptional activity of the complexed DNA. Biochim Biophys Acta. 1353:180–190. 1997. View Article : Google Scholar

26. 

Hui Z, He ZG, Zheng L, Li GY, Shen SR and Li XL: Studies on polyamidoamine dendrimers as efficient gene delivery vector. J Biomater Appl. 22:527–544. 2008.PubMed/NCBI

27. 

Tung CH and Weissleder R: Arginine containing peptides as delivery vectors. Adv Drug Deliv Rev. 55:281–294. 2003. View Article : Google Scholar : PubMed/NCBI

28. 

Futaki S: Arginine-rich peptides: potential for intracellular delivery of macromolecules and the mystery of the translocation mechanisms. Int J Pharm. 245:1–7. 2002. View Article : Google Scholar : PubMed/NCBI

29. 

Torchilin VP, Rammohan R, Weissig V and Levchenko TS: TAT peptide on the surface of liposomes affords their efficient intracellular delivery even at low temperature and in the presence of metabolic inhibitors. Proc Natl Acad Sci USA. 98:8786–8791. 2001. View Article : Google Scholar

30. 

Suzuki T, Futaki S, Niwa M, Tanaka S, Ueda K and Sugiura Y: Possible existence of common internalization mechanisms among arginine-rich peptides. J Biol Chem. 277:2437–2443. 2002. View Article : Google Scholar : PubMed/NCBI

31. 

Choi JS, Nam K, Park JY, Kim JB, Lee JK and Park JS: Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine. J Control Release. 99:445–456. 2004. View Article : Google Scholar : PubMed/NCBI

32. 

Kim JB, Choi JS, Nam K, Lee M, Park JS and Lee JK: Enhanced transfection of primary cortical cultures using arginine-grafted PAMAM dendrimer, PAMAM-Arg. J Control Release. 114:110–117. 2006. View Article : Google Scholar : PubMed/NCBI

33. 

Kumar A, Yellepeddi VK, Davies GE, Strychar KB and Palakurthi S: Enhanced gene transfection efficiency by polyamidoamine (PAMAM) dendrimers modified with ornithine residues. Int J Pharm. 392:294–303. 2010. View Article : Google Scholar : PubMed/NCBI

34. 

Arima H: [Polyfection as nonviral gene transfer method – design of novel nonviral vector using alpha-cyclodextrin]. Yakugaku Zasshi. 124:451–464. 2004.(In Japanese).

35. 

Kang C, Yuan X, Li F, et al: Evaluation of folate-PAMAM for the delivery of antisense oligonucleotides to rat C6 glioma cells in vitro and in vivo. J Biomed Mater Res A. 93:585–594. 2010.PubMed/NCBI

36. 

Kono K, Akiyama H, Takahashi T, Takagishi T and Harada A: Transfection activity of polyamidoamine dendrimers having hydrophobic amino acid residues in the periphery. Bioconjug Chem. 16:208–214. 2005. View Article : Google Scholar : PubMed/NCBI

37. 

Shahin V, Albermann L, Schillers H, et al: Steroids dilate nuclear pores imaged with atomic force microscopy. J Cell Physiol. 202:591–601. 2005. View Article : Google Scholar : PubMed/NCBI

38. 

Choi JS, Ko KS, Park JS, Kim YH, Kim SW and Lee M: Dexamethasone-conjugated poly(amidoamine) dendrimer as a gene carrier for efficient nuclear translocation. Int J Pharm. 320:171–178. 2006. View Article : Google Scholar : PubMed/NCBI

39. 

Huang RQ, Qu YH, Ke WL, Zhu JH, Pei YY and Jiang C: Efficient gene delivery targeted to the brain using a transferrin-conjugated polyethyleneglycol-modified polyamidoamine dendrimer. FASEB J. 21:1117–1125. 2007. View Article : Google Scholar

40. 

Li S, Rizzo MA, Bhattacharya S and Huang L: Characterization of cationic lipid-protamine-DNA (LPD) complexes for intravenous gene delivery. Gene Ther. 5:930–937. 1998. View Article : Google Scholar : PubMed/NCBI

41. 

Tan Y, Whitmore M, Li S, Frederik P and Huang L: LPD nanoparticles – novel nonviral vector for efficient gene delivery. Methods Mol Med. 69:73–81. 2002.

42. 

Sorgi FL, Bhattacharya S and Huang L: Protamine sulfate enhances lipid-mediated gene transfer. Gene Ther. 4:961–968. 1997. View Article : Google Scholar : PubMed/NCBI

43. 

Malik N, Wiwattanapatapee R, Klopsch R, et al: Dendrimers: relationship between structure and biocompatibility in vitro, and preliminary studies on the biodistribution of 125I-labelled polyamidoamine dendrimers in vivo. J Control Release. 65:133–148. 2000.PubMed/NCBI

44. 

Bielinska AU, Chen C, Johnson J and Baker JR Jr: DNA complexing with polyamidoamine dendrimers: implications for transfection. Bioconjug Chem. 10:843–850. 1999. View Article : Google Scholar : PubMed/NCBI

45. 

Wang Y, Kong W, Song Y, et al: Polyamidoamine dendrimers with a modified Pentaerythritol core having high efficiency and low cytotoxicity as gene carriers. Biomacromolecules. 10:617–622. 2009. View Article : Google Scholar : PubMed/NCBI

46. 

Patil ML, Zhang M, Betigeri S, Taratula O, He H and Minko T: Surface-modified and internally cationic polyamidoamine dendrimers for efficient siRNA delivery. Bioconjug Chem. 19:1396–1403. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Shi H and Yin S: Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review). Exp Ther Med 2: 777-781, 2011.
APA
Wang, H., Shi, H., & Yin, S. (2011). Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review). Experimental and Therapeutic Medicine, 2, 777-781. https://doi.org/10.3892/etm.2011.296
MLA
Wang, H., Shi, H., Yin, S."Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review)". Experimental and Therapeutic Medicine 2.5 (2011): 777-781.
Chicago
Wang, H., Shi, H., Yin, S."Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review)". Experimental and Therapeutic Medicine 2, no. 5 (2011): 777-781. https://doi.org/10.3892/etm.2011.296
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Shi H and Yin S: Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review). Exp Ther Med 2: 777-781, 2011.
APA
Wang, H., Shi, H., & Yin, S. (2011). Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review). Experimental and Therapeutic Medicine, 2, 777-781. https://doi.org/10.3892/etm.2011.296
MLA
Wang, H., Shi, H., Yin, S."Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review)". Experimental and Therapeutic Medicine 2.5 (2011): 777-781.
Chicago
Wang, H., Shi, H., Yin, S."Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency (Review)". Experimental and Therapeutic Medicine 2, no. 5 (2011): 777-781. https://doi.org/10.3892/etm.2011.296
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