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Article

Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells

  • Authors:
    • Ping Wang
    • Albert Wingnang Leung
    • Chuanshan Xu
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    Affiliations: Department of Ultrasound Medicine, The Affiliated Hospital of North Sichuan Medical College, Sichuan. P.R. China, School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong SAR, P.R. China
  • Pages: 849-852
    |
    Published online on: July 1, 2011
       https://doi.org/10.3892/etm.2011.317
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Abstract

Ultrasound therapy, as a non-invasive modality, has been attracting extensive attention in the management of malignant tumors. The present study aimed to investigate low-intensity ultrasound-induced cellular destruction and autophagy in nasopharyngeal carcinoma cells in vitro. Nasopharyngeal carcinoma CNE2 cells were subjected to ultrasound exposure, as tumor model cells, at an intensity of 1.35 W/cm2. Cytotoxicity was investigated 24 h after ultrasound treatment. Nuclear damage was observed using nuclear staining with Hoechst 33258. Mitochondrial dysfunction was measured using confocal laser scanning microscopy with rhodamine123 staining. Mitochondrial morphology and autophagy were observed using transmission electron microscopy (TEM). Low-intensity ultrasound significantly killed CNE2 cells proportional to the ultrasonic treatment time. Upon nuclear staining, nuclear condensation and typical apoptotic bodies were noted in the CNE2 cells exposed to ultrasound wave for 12 sec. A collapse in mitochondrial membrane potential was noted in the treated cells. Upon TEM, swollen mitochondria, more vacuoles and autophagy were noted after ultrasound treatment. Our findings demonstrate that low-intensity ultrasound significantly damages CNE2 cells and emphasize that autophagy may be an important event in ultrasound-induced cell death.
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1. 

Lin YT, Wang LF and Hsu YC: Curcuminoids suppress the growth of pharynx and nasopharyngeal carcinoma cells through induced apoptosis. J Agric Food Chem. 57:3765–3770. 2009. View Article : Google Scholar : PubMed/NCBI

2. 

Wang X, Xia X, Xu C, et al: Ultrasound-induced cell death of nasopharyngeal carcinoma cells in the presence of curcumin. Integr Cancer Ther. 10:70–76. 2011. View Article : Google Scholar : PubMed/NCBI

3. 

Wang P, Xu CS, Xu J, Wang X and Leung AW: Hypocrellin B enhances ultrasound-induced cell death of nasopharyngeal carcinoma cells. Ultrasound Med Biol. 36:336–342. 2010. View Article : Google Scholar : PubMed/NCBI

4. 

Wang P, Xu C, Xia X, Xu J, Wang X, Xiang J and Leung AW: Mitochondrial damage in nasopharyngeal carcinoma cells induced by ultrasound radiation in the presence of hypocrellin B. J Ultrasound Med. 29:43–50. 2010.PubMed/NCBI

5. 

Tian Z, Quan X, Xu C, Dan L, Guo H and Leung W: Hematoporphyrin monomethyl ether enhances sonokilling action of ultrasound on osteosarcoma in vivo. J Ultrasound Med. 28:1695–1702. 2009.PubMed/NCBI

6. 

Hundt W, Yuh EL, Steinbach S, Bednarski MD and Guccione S: Comparison of continuous vs. pulsed focused ultrasound in treated muscle tissue as evaluated by magnetic resonance imaging, histological analysis, and microarray analysis. Eur Radiol. 18:993–1004. 2008. View Article : Google Scholar

7. 

Yu T, Wang Z and Mason TJ: A review of research into the uses of low level ultrasound in cancer therapy. Ultrason Sonochem. 11:95–103. 2004. View Article : Google Scholar : PubMed/NCBI

8. 

Alter A, Rozenszajn LA, Miller HI and Rosenschein U: Ultrasound inhibits the adhesion and migration of smooth muscle cells in vitro. Ultrasound Med Biol. 24:711–721. 1998. View Article : Google Scholar : PubMed/NCBI

9. 

Fitzgerald PJ, Takagi A, Moore MP, et al: Intravascular sonotherapy decreases neointimal hyperplasia after stent implantation in swine. Circulation. 103:1828–1831. 2001. View Article : Google Scholar : PubMed/NCBI

10. 

Takeuchi S, Udagawa Y, Oku Y, Fujii T, Nishimura H and Kawashima N: Basic study on apoptosis induction into cancer cells U-937 and EL-4 by ultrasound exposure. Ultrasonics. 44:e345–e348. 2006. View Article : Google Scholar

11. 

Feril LB Jr, Kondo T, Cui ZG, et al: Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line. Cancer Lett. 221:145–152. 2005. View Article : Google Scholar : PubMed/NCBI

12. 

Sasnauskene A, Kadziauskas J, Vezelyte N, Jonusiene VM and Kirveliene V: Apoptosis, autophagy and cell cycle arrest following photodamage to mitochondrial interior. Apoptosis. 14:276–286. 2009. View Article : Google Scholar : PubMed/NCBI

13. 

Yu T, Hu K, Bai J and Wang Z: Reversal of adriamycin resistance in ovarian carcinoma cell line by combination of verapamil and low-level ultrasound. Ultrason Sonochem. 10:37–40. 2003. View Article : Google Scholar : PubMed/NCBI

14. 

Susin SA, Lorenzo HK, Zamzami N, et al: Molecular characterization of mitochondrial apoptosis-inducing factor. Nature. 397:441–446. 1999. View Article : Google Scholar : PubMed/NCBI

15. 

Wang Y, Che C, Chiu J and He Q: Dioscin (saponin)-induced generation of reactive oxygen species through mitochondria dysfunction: a proteomic-based study. J Proteome Res. 6:4703–4710. 2007. View Article : Google Scholar : PubMed/NCBI

16. 

Shen Z, Shen J, Li Q, Chen C, Chen J and Zeng Y: Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide. World J Gastroenterol. 8:31–35. 2002.PubMed/NCBI

17. 

Lee HC, Yin PH, Lu CY, Chi CW and Wei YH: Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells. Biochem J. 348:425–432. 2000. View Article : Google Scholar : PubMed/NCBI

18. 

Li J, Hou N, Faried A, Tsutsumi S, Takeuchi T and Kuwano H: Inhibition of autophagy by 3-MA enhances the effect of 5-FU-induced apoptosis in colon cancer cells. Ann Sur Oncol. 16:761–771. 2009. View Article : Google Scholar : PubMed/NCBI

19. 

Ito S, Koshikawa N, Mochizuki S and Takenaga K: 3-methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinanses independently of autophagy inhibition. Int J Oncol. 31:261–268. 2007.PubMed/NCBI

20. 

Lu Z, Luo RZ, Lu Y, et al: The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J Clin Invest. 118:3917–3929. 2008.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang P, Wingnang Leung A and Xu C: Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells. Exp Ther Med 2: 849-852, 2011.
APA
Wang, P., Wingnang Leung, A., & Xu, C. (2011). Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells. Experimental and Therapeutic Medicine, 2, 849-852. https://doi.org/10.3892/etm.2011.317
MLA
Wang, P., Wingnang Leung, A., Xu, C."Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 2.5 (2011): 849-852.
Chicago
Wang, P., Wingnang Leung, A., Xu, C."Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 2, no. 5 (2011): 849-852. https://doi.org/10.3892/etm.2011.317
Copy and paste a formatted citation
x
Spandidos Publications style
Wang P, Wingnang Leung A and Xu C: Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells. Exp Ther Med 2: 849-852, 2011.
APA
Wang, P., Wingnang Leung, A., & Xu, C. (2011). Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells. Experimental and Therapeutic Medicine, 2, 849-852. https://doi.org/10.3892/etm.2011.317
MLA
Wang, P., Wingnang Leung, A., Xu, C."Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 2.5 (2011): 849-852.
Chicago
Wang, P., Wingnang Leung, A., Xu, C."Low-intensity ultrasound-induced cellular destruction and autophagy of nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 2, no. 5 (2011): 849-852. https://doi.org/10.3892/etm.2011.317
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