Dengue virus presence and surveillance in Okinawa (Review)

Contents

Introduction

History of dengue disease in Okinawa and Japan

Recent condition of dengue disease in Okinawa and the surrounding region

Conclusion

Introduction

A plus-strand RNA virus, the dengue virus (DENV), can be transmitted through mosquito vectors, and these infections lead to dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) (1). DHF and DSS are potentially lethal. The risk of developing them is correlated with infection by one of the four DENV serotypes (DENV1 to DENV4) and having antibodies to another DENV serotype from a previous infection (2). Currently, no specific treatment or vaccine for DENV is available (3).

Recently, reports that DENV has rapidly spread and infected or killed humans have increased (1). In the last 50 years, the incidence has increased by 30-fold. Infection of DENV is estimated at 50 million cases annually (WHO). Among these, Asia and the Pacific region constitute 75% of the current global disease burden (3). As DENV spreads beyond national borders, the emergence of DENV is of particular concern to public health. In this review, we report the current and past conditions of dengue disease in Okinawa.

History of dengue disease in Okinawa and Japan

Okinawa is the southwestern-most prefecture in Japan and consists of a chain of hundreds of islands in the Ryukyu Archipelago, which is over 1,000 km long (Fig. 1). The location is approximately half-way between Kyushu (the southwest region of Japan’s main four islands) and Taiwan. The first reports of dengue disease in Japan were in 1893 and 1903 (4) (Table I). Although the case in 1893 was not diagnosed as dengue disease, the invasion route, epidemic status and disease condition suggest that it was DF. In 1903, Dr Jiro Kawamura diagnosed DF in the Yaeyama Islands. This is thought to be the start of the DF epidemic in Japan (5). Although fever epidemics were frequently found after 1893, that of DF was only found in 1893 and 1904 in the Meiji era (1868–1912). At that time, DF showed weak symptoms and caused a low incidence of death. In addition, severe diseases such as cholera and smallpox hid the DF condition.

Figure 1. Location of the Okinawa prefecture and Taiwan. Okinawa prefecture is located in the southwest region of Japan. An enlarged illustration of Okinawa Islands is shown in the box.

Table I History of major DF epidemics in Okinawa and Japan (4).

Table I

History of major DF epidemics in Okinawa and Japan (4).

Cases	All Japan	Okinawa
1	1893	1893
2	1902	1904
3	1912	1915
4	1916	1931
5	1924
6	1931
7	1934

In July 1903, DF was first diagnosed in the Yaeyama Islands. Subsequently, DF spread to the Miyako Island and the Okinawa main island in September and October, respectively (6). In 1904, DF was still prevalent in Yaeyama and Miyako Islands and the Okinawa main island, and resulted in 61,901 patients and 16 deaths (7,8).

In the Taisho era (1912–1926), the incidence of DF decreased compared to the Meiji era, whereas in 1916 and 1924 DF epidemics were reported (9,10). In the Showa era (1926–1989), DF incidence was found in Japan in 1931, 1934, 1942, 1943 and 1944, whereas there were no incidences after World War II (1939–1945). In Okinawa, a major outbreak of DF was found on Ishigaki Island in 1930. Then, DF spread to the Miyako Island, the Okinawa main island and Kagoshima prefecture on the mainland of Japan (11). After World War II, DF incidence was not found on the main island of Okinawa or the mainland of Japan (12).

In general, the most common invasion route of DF into Okinawa was through the Yaeyama Islands. Then, DF reached the Naha port on the Okinawa main island via the Miyako Island. From Naha, DF spread to other regions of the Okinawa main island and the surrounding islands (13). Therefore, it is reasonable to suggest that most DENV incidents in Okinawa originated from Taiwan, especially in light of mercantile routes of ships during this time. In addition, although Aedes aegypti was previously detected, none was detected after the 1970s in Japan. By contrast, in southern Taiwan, Aedes aegyptia was prevalent. As Taiwan is very close to Okinawa, the continued surveillance for DENV invasion from Taiwan via airplanes and ships is important. Evaluating the distribution of mosquito vectors in this broad region will be useful for the efficient prevention of the appearance and growth of mosquitoes by using chemicals or other methods.

Recent condition of dengue disease in Okinawa and the surrounding region

Two DENV mosquito vectors (Aedes aegypti and Aedes albopictus) were found during the epidemics in the early 20th century in Okinawa and on mainland Japan (14–17). It should be noted that mosquitoes’ eggs remain viable for over a year in dry conditions, while the eggs are deposited in the presence of water (3). This allows the mosquito to re-emerge after a cold winter or dry spell. Previous studies have shown that peak mosquito generation was found in the summer season in a pig livestock barn on Yonaguni Island and a public park in Naha City (18). Therefore, close attention should be paid to the mosquito status during the summer season when invasion of DENV is detected in Okinawa or the surrounding region.

An outbreak of DENV3 occurred in southern Taiwan in 1998 (3,19) and, in 2002, more than 15,000 individuals were infected with DENV in Taiwan. In addition, major dengue outbreaks in Taiwan have been reported (20). Taiwan is located 110 km from Yonaguni Island, which is the last of the Ryukyu Islands chain and the western-most inhabited island of Japan. In epidemiological studies, a wide variety of DENV strains were isolated from imported and indigenous dengue cases during the outbreak of 1981–2006 in Taiwan (20). The results of molecular epidemiology suggested that constant importation of multiple DENVs via mosquitos and individuals from neighboring southeast Asian countries through close commercial links and air travel was responsible for local outbreaks. In Japan, quarantine stations at airports and harbours have performed surveillance for DENV-infected mosquitoes. However, in Okinawa there are US military bases, which the Japanese government cannot control. Therefore, there is the possibility that DENV-infected mosquitoes may invade Okinawa via military airplanes or ships. In addition, the monitoring of migratory wildfowl is necessary, as many birds come to Okinawa in the spring and autumn. In particular, surveillance on Yonaguni Island is important.

Although there are four distinct serotypes (DENV1 to DENV4), molecular analysis during 2003–2007 showed that the geographic distribution of DENV3 strains and genotypes isolated from southeast Asian countries remained unchanged during 2003–2007, whereas the movement and establishment of new DENV1, DENV2 and DENV4 strains were observed in certain areas of Asia (21). Among them, DENV2 and DENV3 genotypes are frequently associated with severe disease accompanying secondary infection, known as antibody-dependent enhancement (2). Therefore, distribution of DENV-infected mosquitoes, especially DENV2 and DENV3, should be observed closely. Thus, effective and sensitive methods for detecting DENV in mosquitoes are required.

Conclusion

Herein, we reviewed the current and past presence of DENV incidence in Okinawa and presented a personal view regarding the future perspectives. During 1838–1955, epidemics of dengue disease were reported 10–20 times in Okinawa. Currently, the incidence of DENV infection has not been reported in Okinawa, and DENV-infected mosquitoes are not resident there. However, several (more than 10) incidences of imported infection cases have been reported annually (22). To prepare for the invasion of DENV, the investigation of resident and generated mosquito vectors in surrounding circumstances should be performed. Although, currently, vector surveillance has focused on airports and harbours, the conditions of public parks, livestock barns and stopovers for migratory birds should be also checked.

Acknowledgements

This study was supported in part by the Japan Science and Technology Agency and the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

References