Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May 2012 Volume 3 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May 2012 Volume 3 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma

  • Authors:
    • Verena Engelstaedter
    • Judith Boda
    • Christine Völklein
    • Jutta Engel
    • Udo Jeschke
    • Thomas Kirchner
    • Doris Mayr
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Ludwig-Maximilians University, D-80337 Munich, Germany, Institute of Pathology, Ludwig-Maximilians University, D-80337 Munich, Germany, Munich Cancer Registry, Ludwig-Maximilians University, D-81377 Munich, Germany
  • Pages: 828-834
    |
    Published online on: February 13, 2012
       https://doi.org/10.3892/etm.2012.481
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Patients with advanced ovarian cancer (FIGO stage III) have a poor clinical prognosis. However, these patients show distinct differences in their survival time, possibly due to differing responses to chemotherapy and differing tumor biology. In contrast to histological subtype, grading and staging, which are known to affect a patient's prognosis, the impact of the human epidermal growth factor receptor 2 (Her-2/neu), topoisomerase IIα and epidermal growth factor receptor (EGFR) on survival remain inconclusive. Therefore, the aim of this study was to assess their impact on survival in a group of advanced ovarian cancer patients. Tissue microarrays were constructed from specimens of 243 patients. Gene copy and chromosome numbers were evaluated by fluorescence in situ hybridization (FISH) and protein expression by immunohistochemistry (IHC). Scoring for the latter was calculated by considering the percentage of positive tumor cells and the relative staining intensity. FISH results were evaluated by previously published recommendations and correlated with overall survival. Using IHC, 1.6% of the cases that were tested for Her-2/neu and topoisomerase IIα were strongly positive, and 12.3% were positive for EGFR. Using FISH, 4.4% amplifications and 2.1% polysomies for Her-2/neu were identified; topoisomerase IIα showed 2.2% amplifications, 0.4% deletions and 3.5% polysomies. We observed 10.8% high polysomies, but no amplification for EGFR. None of the results obtained by IHC or FISH correlated with overall survival. In general, Her-2/neu, topoisomerase IIα and EGFR may be prognostic factors in ovarian carcinomas. However, within this group of FIGO stage III patients, differences in gene aberration or protein expression were not able to predict differences in survival.
View Figures
View References

1. 

Robert Koch-Institut und die Gesellschaft der epidemiologischen Krebsregister in Deutschland e. V. Berlin. Krebs in Deutschland 2003–2004 Häufigkeit und Trends. 6th edition. 2008

2. 

Statistisches Bundesamt: Todesursachenstatistik in Deutschland. Fachserie 12, Reihe 4,. pp. 1–114. 2008

3. 

Deutsche Krebsgesellschaft e. V. Krebshäufigkeit - die aktuellen Zahlen,. 2007

4. 

Dietel M: Ovar: tumoren. Pathologie. Remmele W: 4. 2nd edition. Springer-Verlag; Berlin Heidelberg: pp. 92–123. 1992

5. 

Olsen CM, Cnossen J, Green AC, et al: Comparison of symptoms and presentation of women with benign, low malignant potential and invasive ovarian tumors. Eur J Gynaecol Oncol. 28:376–380. 2007.PubMed/NCBI

6. 

Cannistra SA: Cancer of the ovary. N Engl J Med. 351:2519–2529. 2004. View Article : Google Scholar : PubMed/NCBI

7. 

Goff BA, Muntz HG, Greer BE, et al: Oncogene expression: long-term compared with short-term survival in patients with advanced epithelial ovarian cancer. Obstet Gynecol. 92:88–93. 1998. View Article : Google Scholar : PubMed/NCBI

8. 

Kaern J, Aghmesheh M, Nesland JM, et al: Prognostic factors in ovarian carcinomas stage III patients. Can biomarkers improve the prediction of short- and long-term survivors? Int J Gynecol Cancer. 15:1014–1022. 2005. View Article : Google Scholar : PubMed/NCBI

9. 

Schindlbeck C, Hantschmann P, Zerzer M, et al: Prognostic impact of KI67, p53, human epithelial growth factor receptor 2, topoisomerase IIalpha, epidermal growth factor receptor, and nm23 expression of ovarian carcinomas and disseminated tumor cells in the bone marrow. Int J Gynecol Cancer. 17:1047–1055. 2007. View Article : Google Scholar

10. 

Faggad A, Darb-Esfahani S, Wirtz R, et al: Topoisomerase IIalpha mRNA and protein expression in ovarian carcinoma: correlation with clinicopathological factors and prognosis. Mod Pathol. 22:579–588. 2009. View Article : Google Scholar : PubMed/NCBI

11. 

De Graeff P, Crijns AP, de Jong S, et al: Modest effect of p53, EGFR and Her-2/neu on prognosis in epithelial ovarian cancer: a meta-analysis. Br J cancer. 101:149–159. 2009.PubMed/NCBI

12. 

Wang JC: Cellular roles of DNA topoisomerases: a molecular perspective. Nat Rev Mol Cell Biol. 3:430–440. 2002. View Article : Google Scholar : PubMed/NCBI

13. 

World Health Organization: Pathology & Genetics. Tumours of the Breast and Female Genital Organs. IARC Press; Lyon: 119. 2003

14. 

Wolff AC, Hammond ME, Schwartz JN, et al: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med. 131:18–43. 2007.

15. 

Remmele W and Stegner HE: Recommendation for uniform definition of an immunoreactive score (IRS) for immunohisto-chemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologe. 8:138–140. 1987.(In German).

16. 

Chekerov R, Klaman I, Zafrakas M, et al: Altered expression pattern of topoisomerase IIα in ovarian tumor epithelial and stromal cells after platinum-based chemotherapy. Neoplasia. 8:38–45. 2006.

17. 

Pauletti G, Godolphin W, Press MF, et al: Detection and quantitation of HER-2/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization. Oncogene. 13:63–72. 1996.PubMed/NCBI

18. 

Cappuzzo F, Hirsch FR, Rossi E, et al: Epidermal growth factor receptor gene and protein and gefitinib sensitivity in non-small-cell lung cancer. J Natl Cancer Inst. 97:643–655. 2005. View Article : Google Scholar : PubMed/NCBI

19. 

Serrano-Olvera A, Duenas-Gonzalez A, Gallardo-Rincón D, et al: Prognostic, predictive and therapeutic implications of HER2 in invasive epithelial ovarian cancer. Cancer Treat Rev. 32:180–190. 2006. View Article : Google Scholar : PubMed/NCBI

20. 

Lee ES, Lee Y, Suh D, et al: Detection of HER-2 and EGFR gene amplification using chromogenic in-situ hybridization technique in ovarian tumors. Appl Immunohistochem Mol Morphol. 18:69–74. 2010. View Article : Google Scholar : PubMed/NCBI

21. 

Vermeij J, Teugels E, Bourgain C, et al: Genomic activation of the EGFR and HER2-neu genes in a significant proportion of invasive epithelial ovarian cancers. BMC cancer. 8:32008. View Article : Google Scholar : PubMed/NCBI

22. 

Dimova I, Raicheva S, Dimitrov R, et al: Coexistence of copy number increases of c-Myc, ZNF217, CCND1, ErbB1 and ErbB2 in ovarian cancers. Onkologie. 32:405–410. 2009. View Article : Google Scholar : PubMed/NCBI

23. 

De Graeff P, Crijns AP, Ten Hoor HK, et al: The ErbB signalling pathway: protein expression and prognostic value in epithelial ovarian cancer. Br J Cancer. 99:341–349. 2008.PubMed/NCBI

24. 

Farley J, Fuchiuji S, Darcy KM, et al: Associations between ERBB2 amplification and progression-free survival and overall survival in advanced stage, suboptimally-resected epithelial ovarian cancers: a Gynecologic Oncology Group Study. Gynecol Oncol. 113:341–347. 2009. View Article : Google Scholar

25. 

Durbecq V, Paesmans M, Cardoso F, et al: Topoisomerase-II alpha expression as a predictive marker in a population of advanced breast cancer patients randomly treated either with single-agent doxorubicin or single-agent docetaxel. Mol Cancer Ther. 3:1207–1214. 2004.

26. 

Schindlbeck C, Mayr D, Olivier C, et al: Topoisomerase IIalpha expression rather than gene amplification predicts responsiveness of adjuvant anthracycline-based chemotherapy in women with primary breast cancer. J Cancer Res Clin Oncol. 136:1029–1037. 2010. View Article : Google Scholar

27. 

Lassus H, Sihto H, Leminen A, et al: Gene amplification, mutation, and protein expression of EGFR and mutations of ERBB2 in serous ovarian carcinoma. J Mol Med. 84:671–681. 2006. View Article : Google Scholar : PubMed/NCBI

28. 

Tanaka Y, Terai Y, Tanabe A, et al: Prognostic effect of epidermal growth factor receptor gene mutations and the aberrant phosphorylation of Akt and ERK in ovarian cancer. Cancer Biol Ther. 11:50–57. 2011. View Article : Google Scholar : PubMed/NCBI

29. 

Stadlmann S, Gueth U, Reiser U, et al: Epithelial growth factor receptor status in primary and recurrent ovarian cancer. Mod Pathol. 19:607–610. 2006. View Article : Google Scholar : PubMed/NCBI

30. 

Tanner B, Hasenclever D, Stern K, et al: ErbB-3 predicts survival in ovarian cancer. J Clin Oncol. 24:4317–4323. 2006. View Article : Google Scholar : PubMed/NCBI

31. 

Garcia-Velasco A, Mendiola C, Sanchez-Munoz A, et al: Prognostic value of hormonal receptors, p53, ki67 and Her2/neu expression in epithelial ovarian carcinoma. Clin Transl Oncol. 10:367–371. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Engelstaedter V, Boda J, Völklein C, Engel J, Jeschke U, Kirchner T and Mayr D: Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma. Exp Ther Med 3: 828-834, 2012.
APA
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., & Mayr, D. (2012). Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma. Experimental and Therapeutic Medicine, 3, 828-834. https://doi.org/10.3892/etm.2012.481
MLA
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., Mayr, D."Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma". Experimental and Therapeutic Medicine 3.5 (2012): 828-834.
Chicago
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., Mayr, D."Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma". Experimental and Therapeutic Medicine 3, no. 5 (2012): 828-834. https://doi.org/10.3892/etm.2012.481
Copy and paste a formatted citation
x
Spandidos Publications style
Engelstaedter V, Boda J, Völklein C, Engel J, Jeschke U, Kirchner T and Mayr D: Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma. Exp Ther Med 3: 828-834, 2012.
APA
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., & Mayr, D. (2012). Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma. Experimental and Therapeutic Medicine, 3, 828-834. https://doi.org/10.3892/etm.2012.481
MLA
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., Mayr, D."Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma". Experimental and Therapeutic Medicine 3.5 (2012): 828-834.
Chicago
Engelstaedter, V., Boda, J., Völklein, C., Engel, J., Jeschke, U., Kirchner, T., Mayr, D."Lack of prognostic relevance of Her-2/neu, topoisomerase IIα and EGFR in advanced ovarian carcinoma". Experimental and Therapeutic Medicine 3, no. 5 (2012): 828-834. https://doi.org/10.3892/etm.2012.481
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team