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Article

Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene

  • Authors:
    • Hitoshi Yamazaki
    • Tsuyoshi Chijiwa
    • Yoshimasa Inoue
    • Yoshiyuki Abe
    • Hiroshi Suemizu
    • Kenji Kawai
    • Masatoshi Wakui
    • Daisuke Furukawa
    • Masaya Mukai
    • Sadahito Kuwao
    • Makoto Saegusa
    • Masato Nakamura
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan, Japan Self Defense Force Hospital Naha, Okinawa, Japan, Department of Surgery, Tokai University Hachioji Hospital, Tokyo, Japan, Tokorozawa PET Diagnostic Imaging Clinic, Saitama, Japan, Central Institute for Experimental Animals, Kanagawa, Japan, Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan, Department of Surgery, Tokai University School of Medicine, Kanagawa, Japan, Department of Pathology and Cytology, Higashi-Yamato Hospital, Tokyo, Japan, Regenerative Medicine and Pathology, Tokai University School of Medicine, Kanagawa, Japan
  • Pages: 793-796
    |
    Published online on: February 28, 2012
       https://doi.org/10.3892/etm.2012.497
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Abstract

Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As a consequence, the translation of these mRNAs is inhibited or they are destabilized resulting in downregulation of the encoded protein. The microRNA-34 (miR-34) family, which comprises three processed microRNAs (miR-34a/b/c) was identified as the mediator of tumor suppression by p53. Many reports suggest that the miR-34s contribute to the inhibition of invasion or metastasis in various tumor types. In this study, we evaluated the expression of the miR-34 family in four human melanoma cell lines (A375, G361, C32TG and SK-MEL-24) which have the wild-type p53 gene using real-time reverse transcription PCR. We also examined their generative and invasive characteristics using the cell proliferation assay and the invasion/migration assay, respectively. All four melanoma cell lines showed significant expression of miR-34s – A375: miR-34a 0.6176, miR-34b 0.7625, miR-34c 0.7877; G361: 7.6424, 16.4127, 22.0332; C32TG: 2.1630, 2.1091, 8.4425; SK-MEL‑24: 0.3621, 2.5659, 8.5907. The cell doubling times of these cell lines in h:min were as follows: A375 23:23, G361 68:24, C32TG 47:22 and SK-MEL-24 67:03. The in vitro generation times of G361 and SK-MEL-24, which showed increased expression of miR-34c, were significantly shorter than A375 with decreased expression of miR-34c (p=0.0063, ANOVA). Invasion (%) of the four cell lines was as follows: A375 44.0%, G361 22.4%, C32TG 13.8% and SK-MEL-24 45.0%. In vitro invasiveness of G361 and C32TG, which showed increased expression of miR-34a, was significantly suppressed (p=0.005, ANOVA). These results suggest that overexpression of miR-34a and c suppresses invasive and generative potentials, respectively, in human malignant melanoma.
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1. 

Hermeking H: p53 enters the microRNA world. Cancer Cell. 12:414–418. 2007. View Article : Google Scholar : PubMed/NCBI

2. 

Fabbri M, Croce CM and Calin GA: MicroRNAs. Cancer J. 14:1–6. 2008. View Article : Google Scholar

3. 

Mueller DW and Bosserhoff AK: Role of miRNAs in the progression of malignant melanoma. Br J Cancer. 101:551–556. 2009. View Article : Google Scholar : PubMed/NCBI

4. 

Yamashita T, Tokino T, Tonoki H, et al: Induction of apoptosis in melanoma cell lines by p53 and its related proteins. J Invest Dermatol. 117:914–919. 2001. View Article : Google Scholar : PubMed/NCBI

5. 

Min FL, Zhang H, Li WJ, Gao QX and Zhou GM: Effect of exogenous wild-type p53 on melanoma cell death pathways induced by irradiation at different linear energy transfer. In Vitro Cell Dev Biol Anim. 41:284–288. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Miyato Y and Ando K: Apoptosis of human melanoma cells by a combination of lonidamine and radiation. J Radiat Res (Tokyo). 45:189–194. 2004. View Article : Google Scholar : PubMed/NCBI

7. 

Monney L, Sabatos CA, Gaglia JL, et al: Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature. 415:536–541. 2002. View Article : Google Scholar : PubMed/NCBI

8. 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

9. 

Chijiwa T, Abe Y, Ikoma N, et al: Thrombospondin 2 inhibits metastasis of human malignant melanoma through microenvironment-modification in NOD/SCID/γCnull (NOG) mice. Int J Oncol. 34:5–13. 2009.PubMed/NCBI

10. 

Ikoma N, Yamazaki H, Abe Y, et al: S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/γCnull (NOG) mouse model. Oncol Rep. 14:633–637. 2005.PubMed/NCBI

11. 

Dominguez-Sola D, Ying CY, Grandori C, et al: Non-transcriptional control of DNA replication by c-Myc. Nature. 448:445–451. 2007. View Article : Google Scholar : PubMed/NCBI

12. 

Ho J and Benchimol S: Transcriptional repression mediated by the p53 tumour suppressor. Cell Death Differ. 10:404–408. 2003. View Article : Google Scholar : PubMed/NCBI

13. 

Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–103. 2007. View Article : Google Scholar : PubMed/NCBI

14. 

Kloosterman WP and Plasterk RH: The diverse functions of microRNAs in animal development and disease. Dev Cell. 11:441–450. 2006. View Article : Google Scholar : PubMed/NCBI

15. 

Valencia-Sanchez MA, Liu J, Hannon GJ and Parker R: Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515–524. 2006. View Article : Google Scholar : PubMed/NCBI

16. 

Bommer GT, Gerin I, Feng Y, et al: p53-mediated activation of miRNA34 candidate tumor-suppressor genes. Curr Biol. 17:1298–1307. 2007. View Article : Google Scholar : PubMed/NCBI

17. 

Chang TC, Wentzel EA, Kent OA, et al: Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis. Mol Cell. 26:745–752. 2007. View Article : Google Scholar : PubMed/NCBI

18. 

Corney DC, Flesken-Nikitin A, Godwin AK, Wang W and Nikitin AY: MicroRNA-34b and microRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth. Cancer Res. 67:8433–8438. 2007. View Article : Google Scholar : PubMed/NCBI

19. 

He L, He X, Lim LP, et al: A microRNA component of the p53 tumour suppressor network. Nature. 447:1130–1134. 2007. View Article : Google Scholar : PubMed/NCBI

20. 

Raver-Shapira N, Marciano E, Meiri E, et al: Transcriptional activation of miR-34a contributes to p53-mediated apoptosis. Mol Cell. 26:731–743. 2007. View Article : Google Scholar : PubMed/NCBI

21. 

Tarasov V, Jung P, Verdoodt B, et al: Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrest. Cell Cycle. 6:1586–1593. 2007. View Article : Google Scholar : PubMed/NCBI

22. 

Tazawa H, Tsuchiya N, Izumiya M and Nakagama H: Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells. Proc Natl Acad Sci USA. 104:15472–15477. 2007. View Article : Google Scholar : PubMed/NCBI

23. 

Bagchi A and Mills AA: The quest for the 1p36 tumor suppressor. Cancer Res. 68:2551–2556. 2008. View Article : Google Scholar : PubMed/NCBI

24. 

Zhou Z, Flesken-Nikitin A, Corney DC, et al: Synergy of p53 and Rb deficiency in a conditional mouse model for metastatic prostate cancer. Cancer Res. 66:7889–7898. 2006. View Article : Google Scholar : PubMed/NCBI

25. 

Sun F, Fu H, Liu Q, et al: Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest. FEBS Lett. 582:1564–1568. 2008. View Article : Google Scholar : PubMed/NCBI

26. 

Pigazzi M, Manara E, Baron E and Basso G: miR-34b targets cyclic AMP-responsive element binding protein in acute myeloid leukemia. Cancer Res. 69:2471–2478. 2009. View Article : Google Scholar : PubMed/NCBI

27. 

Bemis LT, Chen R, Amato CM, et al: MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. Cancer Res. 68:1362–1368. 2008. View Article : Google Scholar : PubMed/NCBI

28. 

Segura MF, Hanniford D, Menendez S, et al: Aberrant miR-182 expression promotes melanoma metastasis by repressing FOXO3 and microphthalmia-associated transcription factor. Proc Natl Acad Sci USA. 106:1814–1819. 2009. View Article : Google Scholar : PubMed/NCBI

29. 

Lodygin D, Tarasov V, Epanchintsev A, et al: Inactivation of miR-34a by aberrant CpG methylation in multiple types of cancer. Cell Cycle. 7:2591–2600. 2008. View Article : Google Scholar : PubMed/NCBI

30. 

Migliore C, Petrelli A, Ghiso E, et al: MicroRNAs impair MET-mediated invasive growth. Cancer Res. 68:10128–10136. 2008. View Article : Google Scholar : PubMed/NCBI

31. 

Yan D, Zhou X, Chen X, et al: MicroRNA-34a inhibits uveal melanoma cell proliferation and migration through downregulation of c-Met. Invest Ophthalmol Vis Sci. 50:1559–1565. 2009. View Article : Google Scholar : PubMed/NCBI

32. 

Fujita Y, Kojima K, Hamada N, et al: Effects of miR-34a on cell growth and chemoresistance in prostate cancer PC3 cells. Biochem Biophys Res Commun. 377:114–119. 2008. View Article : Google Scholar : PubMed/NCBI

33. 

Kozaki K, Imoto I, Mogi S, Omura K and Inazawa J: Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res. 68:2094–2105. 2008. View Article : Google Scholar : PubMed/NCBI

34. 

Toyota M, Suzuki H, Sasaki Y, et al: Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer. Cancer Res. 68:4123–4132. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yamazaki H, Chijiwa T, Inoue Y, Abe Y, Suemizu H, Kawai K, Wakui M, Furukawa D, Mukai M, Kuwao S, Kuwao S, et al: Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene. Exp Ther Med 3: 793-796, 2012.
APA
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K. ... Nakamura, M. (2012). Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene. Experimental and Therapeutic Medicine, 3, 793-796. https://doi.org/10.3892/etm.2012.497
MLA
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K., Wakui, M., Furukawa, D., Mukai, M., Kuwao, S., Saegusa, M., Nakamura, M."Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene". Experimental and Therapeutic Medicine 3.5 (2012): 793-796.
Chicago
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K., Wakui, M., Furukawa, D., Mukai, M., Kuwao, S., Saegusa, M., Nakamura, M."Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene". Experimental and Therapeutic Medicine 3, no. 5 (2012): 793-796. https://doi.org/10.3892/etm.2012.497
Copy and paste a formatted citation
x
Spandidos Publications style
Yamazaki H, Chijiwa T, Inoue Y, Abe Y, Suemizu H, Kawai K, Wakui M, Furukawa D, Mukai M, Kuwao S, Kuwao S, et al: Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene. Exp Ther Med 3: 793-796, 2012.
APA
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K. ... Nakamura, M. (2012). Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene. Experimental and Therapeutic Medicine, 3, 793-796. https://doi.org/10.3892/etm.2012.497
MLA
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K., Wakui, M., Furukawa, D., Mukai, M., Kuwao, S., Saegusa, M., Nakamura, M."Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene". Experimental and Therapeutic Medicine 3.5 (2012): 793-796.
Chicago
Yamazaki, H., Chijiwa, T., Inoue, Y., Abe, Y., Suemizu, H., Kawai, K., Wakui, M., Furukawa, D., Mukai, M., Kuwao, S., Saegusa, M., Nakamura, M."Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene". Experimental and Therapeutic Medicine 3, no. 5 (2012): 793-796. https://doi.org/10.3892/etm.2012.497
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