Development of a new auxiliary heterotopic partial liver transplantation technique using a liver cirrhosis model in minipigs: Preliminary report of eight transplants

Zhang,Jun-Jing; Niu,Jian-Xiang; Yue,Gen-Quan; Zhong,Hai-Yan; Meng,Xing-Kai

doi:10.3892/etm.2012.507

May 2012 Volume 3 Issue 5

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May 2012 Volume 3 Issue 5

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Article

Development of a new auxiliary heterotopic partial liver transplantation technique using a liver cirrhosis model in minipigs: Preliminary report of eight transplants

Authors:
- Jun-Jing Zhang
- Jian-Xiang Niu
- Gen-Quan Yue
- Hai-Yan Zhong
- Xing-Kai Meng
View Affiliations / Copyright

Affiliations: Department of General Surgery, XuanWu Hospital, Capital Medical University, Peking 100053, P.R. China, Department of General Surgery, The Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010050, P.R. China, Department of Anaesthesia, The Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010050, P.R. China
Pages: 865-868
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Published online on: March 7, 2012

https://doi.org/10.3892/etm.2012.507
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Abstract

This study aimed to develop a new auxiliary heterotopic partial liver transplantation (AHPLT) technique in minipigs using a model of liver cirrhosis. Based on our previous study, 14 minipigs were induced to cirrhosis by administration of carbon tetrachloride (CCl4) through intraperitoneal injection. All of the cirrhotic animals were utilized as recipients. The donor's liver was placed on the recipient's splenic bed, and the anastomosis was performed as follows: end-to-end anastomosis between the donor's portal vein and the recipient's splenic vein, end-to-side anastomosis between the donor's suprahepatic vena cava and the recipient's suprahepatic vena cava, and end-to-end anastomosis between the donor's hepatic artery and the recipient's splenic artery. The common bile duct of the donor was intubated and bile was collected with an extracorporeal bag. Vital signs, portal vein pressure (PVP), hepatic venous pressure (HVP) and portal vein pressure gradient (PVPG) were monitored throughout the transplantation. All 8 minipigs that developed liver cirrhosis were utilized to establish the new AHPLT; 7 cases survived. Following the surgical intervention, the PVP and PVPG of the recipients were lower than those prior to the operation (P<0.05), whereas the PVP and PVPG of the donors increased significantly compared to those of the normal animals (P<0.05). A new operative technique for AHPLT has been successfully described herein using a model of liver cirrhosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Development of a new auxiliary heterotopic partial liver transplantation technique using a liver cirrhosis model in minipigs: Preliminary report of eight transplants

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