Effect of Jagged1 on the proliferation and migration of colon cancer cells
- Authors:
- Yong Tan
- Jingjing Peng
- Dong Wei
- Peng Chen
- Yu Zhao
View Affiliations
Affiliations: Department of Oncology, The General Hospital of Chengdu Military Region, Chengdu 610083, P.R. China
- Published online on: April 17, 2012 https://doi.org/10.3892/etm.2012.549
-
Pages:
89-92
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
The aim of this study was to determine the effect of Jagged1 on the proliferation and migration of colon cancer cells. HT-29 colorectal carcinoma cells were transfected with adenovirus carrying either the Jagged1 gene (Ad-Jagged1) or Jagged1 small interfering RNA (siRNA) (Ad-si/hJagged1). The protein was detected using western blot analysis. The proliferation of cells was observed by assessing the incorporation of [3H]-thymidine. The migration of cells was detected using a modified Boyden chamber assay. The level of Jagged1 protein increased by 2.8-fold with Ad-Jagged1 transfection and decreased by 70% with Ad-si/hJagged1 transfection. Proliferation, migration and p-Akt protein expression were enhanced in the Ad-Jagged1-transfected group compared to that in the non-transfected control group. In addition, the proliferation, migration and phosphorylated Akt protein expression were reduced in the Ad-si/hJagged1-transfected group compared to that in the non-transfected control group. The Jagged1 protein may promote proliferation and migration of colon cancer cells, which may be related to its effect on Akt phosphorylation.
View References
|
1
|
Peto J: Cancer epidemiology in the last
century and the next decade. Nature. 411:390–395. 2001. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Liao W, Li G and Chen J: Expression of
Notch-1 and Jagged-1 protein in human colorectal cancer and its
significance. J Shandong Med. 50:12–14. 2010.
|
|
3
|
Farnie G and Clarke RB: Mammary stem cells
and breast cancer: role of Notch signalling. Stem Cell Rev.
3:169–175. 2007. View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Gongora C, Candeil L, Vezzio N, et al:
Altered expression of cell proliferation related and interferon
stimulated genes in colon cancer cells resistant to SN38. Cancer
Biol Ther. 7:822–832. 2008. View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Shafaee Z, Schmidt H, Du W, et al:
Cyclopamine increases the cytotoxic effects of paclitaxel and
radiation but not cisplatin and gemcitabine in Hedgehog expressing
pancreatic cancer cells. Cancer Chemother Pharmacol. 58:765–770.
2006. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Kaplan RN, Riba RD, Zacharoulis S, et al:
VEGFR1-positive haematopoietic bone marrow progenitors initiate the
pre-metastatic niche. Nature. 4380:820–827. 2005. View Article : Google Scholar : PubMed/NCBI
|
|
7
|
Shimojo H, Ohtsuka T and Kageyama R:
Oscillations in notch signaling regulate maintenance of neural
progenitors. Neuron. 58:52–64. 2008. View Article : Google Scholar : PubMed/NCBI
|
|
8
|
Wickenden JA and Watson CJ: Key signaling
nodes in mammary gland development and cancer. Signalling
downstream of PI3 kinase in mammary epithelium: a play in 3 Akts.
Breast Cancer Res. 12:202–204. 2010. View
Article : Google Scholar : PubMed/NCBI
|
|
9
|
Priulla M, Calastretti IA, Bruno P, et al:
Preferential chemosensitization of PTEN mutated prostate cells by
silencing the Akt kinase. Prostate. 67:782–789. 2007. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Zhang D and Fan D: Multidrug resistance in
gastric cancer: recent research advances and ongoing therapeutic
challenges. Expert Rev Anticancer Ther. 7:1369–1378. 2007.
View Article : Google Scholar : PubMed/NCBI
|
