Adrenal tumor volume in a genetically engineered mouse model of neuroblastoma determined by magnetic resonance imaging

Kawano,Kumi; Hattori,Yoshiyuki; Iwakura,Hiroshi; Akamizu,Takashi; Maitani,Yoshie

doi:10.3892/etm.2012.564

July 2012 Volume 4 Issue 1

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July 2012 Volume 4 Issue 1

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Article

Adrenal tumor volume in a genetically engineered mouse model of neuroblastoma determined by magnetic resonance imaging

Authors:
- Kumi Kawano
- Yoshiyuki Hattori
- Hiroshi Iwakura
- Takashi Akamizu
- Yoshie Maitani
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Affiliations: Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan, Medical Innovation Center, Kyoto University Graduate School of Medicine, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8501, Japan, The First Department of Medicine, Wakayama Medical University, Wakayama 641-8509, Japan
Pages: 61-64
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Published online on: May 2, 2012

https://doi.org/10.3892/etm.2012.564
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Abstract

Neuroblastoma is the second most common type of solid tumor in children and is commonly found in the adrenal medulla. Recently, we developed transgenic mice exhibiting tumors bilaterally in the adrenal medulla through the expression of SV40 T-antigen. Since these transgenic mice facilitate the development of new therapeutic approaches for neuroblastoma, non-invasive monitoring methods are required for serial measurement of tumor progression. In this study, we monitored the serial progression of adrenal tumors in transgenic mice by magnetic resonance imaging (MRI) of 9.4 T vertical type, and calculated the tumor volume. The accuracy of the tumor volume determination by MRI was verified by standard volume measurements at autopsy. Adrenal tumors as small as 1.5 mm in diameter were detected and quantitatively measured in the transgenic mice by in vivo MRI without using exogenous contrast agents on T2-weighted spin echo images. The tumor sizes by MRI correlated better with tumor weight than the volume by calculation with a caliper. Furthermore, we monitored the change of tumor volume following administration of doxorubicin at weekly intervals. The tumor progression and regression following doxorubicin treatment in the individual mice could be observed by serial MRI. From these findings, non-invasive MRI is likely to be useful for monitoring the response of spontaneous tumors to therapeutic drugs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Adrenal tumor volume in a genetically engineered mouse model of neuroblastoma determined by magnetic resonance imaging

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