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Article

Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells

  • Authors:
    • Dong-Yin Li
    • Chuan Gu
    • Jun Min
    • Zhong-Hua Chu
    • Qing-Jia Ou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Tianjin First Central Hospital, Tianjin 300192, P.R. China, Department of Hepatobiliary Surgery, The Second Hospital, Sun Yat-sen Hospital, Guangzhou 510120, P.R. China
  • Pages: 131-134
    |
    Published online on: May 2, 2012
       https://doi.org/10.3892/etm.2012.565
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Abstract

The aim of the present study was to explore an optimal method for maturation induction of dendritic cells (DCs). Human monocyte-derived DCs were induced in the presence of GM-CSF and IL-4. On Day 6, the maturation of DCs was induced with CD40L, LPS, TNF‑α and cocktail of cytokines (TNF-α, IL-6, IL-1β and PGE2), respectively, for 24 h. Then, DCs were harvested and subjected to flow cytometry (FCM) for the detection of CD80, CD83, CD86 and HLA-DR. FITC-dextran endocytic activity was measured by FCM, IL-12 production by ELISA and T lymphocyte proliferation following DC stimulation by MTT assay. CD40L, LPS, TNF-α and a cocktail of cytokines induced DC maturation. Induction with the cocktail of cytokines was the most efficient, and the expression rate of CD83 was 66.91% (P<0.05). The FITC-dextran endocytic activity of mature DCs was significantly reduced, and IL-12 production was dramatically increased in mature DCs, particularly in those following induction using the cocktail of cytokines. The mature DCs had potent ability to stimulate the proliferation of lymphocytes. The cocktail of cytokines is a favorable strategy for the induction of DC maturation.
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1 

Reichardt VL, Brossart P and Kanz L: Dendritic cells in vaccination therapies of human malignant disease. Blood Rev. 18:235–243. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Chapuis F, Rosenzwajg M, Yagello M, Ekman M, Biberfeld P and Gluckman JC: Differentiation of human dendritic cells from monocytes in vitro. Eur J Immunol. 27:431–441. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Banchereau J and Palucka AK: Dendritic cells as therapeutic vaccines against cancer. Nat Rev Immunol. 5:296–306. 2005. View Article : Google Scholar : PubMed/NCBI

4 

John J, Hutchinson J, Dalgleish A and Pandha HL: Cryopreservation of immature monocyte-derived dendritic cells results in enhanced cell maturation but reduced endocytic activity and efficiency of adenoviral transduction. J Immunol Methods. 272:35–48. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Steinman RM and Cohn ZA: Identification of a novel cell type in peripheral lymphoid organs of mice. I Morphology, quantitation, tissue distribution. J Exp Med. 137:1142–1162. 1973. View Article : Google Scholar

6 

Sheng KC, Pietersz GA, Wright MD and Apostolopoulos V: Dendritic cells: activation and maturation - applications for cancer immunotherapy. Curr Med Chem. 12:1783–1800. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Brossart P, Grünebach F, Stuhler G, Reichardt VL, Möhle R, Kanz L, et al: Generation of functional human dendritic cells from adherent peripheral blood monocytes by CD40 ligation in the absence of granulocyte-macrophage colony-stimulating factor. Blood. 92:4238–4247. 1998.

8 

Reddy A, Sapp M, Feldman M, Subklewe M and Bhardwaj N: A monocyte conditioned medium is more effective than defined cytokines in mediating the terminal maturation of human dendritic cells. Blood. 90:3640–3646. 1997.PubMed/NCBI

9 

Jonuleit H, Kühn U, Müller G, Steinbrink K, Paragnik L, Schmitt E, et al: Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur J Immunol. 27:3135–3142. 1997. View Article : Google Scholar

10 

Höltl L, Rieser C, Papesh C, Ramoner R, Herold M, Klocker H, et al: Cellular and humoral immune responses in patients with metastatic renal cell carcinoma after vaccination with antigen pulsed dendritic cells. J Urol. 161:777–782. 1999.

11 

Lee AW, Truong T, Bickham K, Fonteneau JF, Larsson M, Da Silva I, et al: A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of human monocyte-derived dendritic cells: implications for immunotherapy. Vaccine. 20(Suppl 4): A8–A22. 2002.PubMed/NCBI

12 

Berger TG, Feuerstein B, Strasser E, Hirsch U, Schreiner D, Schuler G, et al: Large-scale generation of mature monocyte-derived dendritic cells for clinical application in cell factories. J Immunol Methods. 268:131–140. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li D, Gu C, Min J, Chu Z and Ou Q: Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells. Exp Ther Med 4: 131-134, 2012.
APA
Li, D., Gu, C., Min, J., Chu, Z., & Ou, Q. (2012). Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells. Experimental and Therapeutic Medicine, 4, 131-134. https://doi.org/10.3892/etm.2012.565
MLA
Li, D., Gu, C., Min, J., Chu, Z., Ou, Q."Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells". Experimental and Therapeutic Medicine 4.1 (2012): 131-134.
Chicago
Li, D., Gu, C., Min, J., Chu, Z., Ou, Q."Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells". Experimental and Therapeutic Medicine 4, no. 1 (2012): 131-134. https://doi.org/10.3892/etm.2012.565
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Gu C, Min J, Chu Z and Ou Q: Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells. Exp Ther Med 4: 131-134, 2012.
APA
Li, D., Gu, C., Min, J., Chu, Z., & Ou, Q. (2012). Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells. Experimental and Therapeutic Medicine, 4, 131-134. https://doi.org/10.3892/etm.2012.565
MLA
Li, D., Gu, C., Min, J., Chu, Z., Ou, Q."Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells". Experimental and Therapeutic Medicine 4.1 (2012): 131-134.
Chicago
Li, D., Gu, C., Min, J., Chu, Z., Ou, Q."Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells". Experimental and Therapeutic Medicine 4, no. 1 (2012): 131-134. https://doi.org/10.3892/etm.2012.565
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