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Article

Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model

  • Authors:
    • İdris Nergiz
    • Barbaros Başeskioğlu
    • Aydın Yenilmez
    • Nilüfer Erkasap
    • Cavit Can
    • Murat Tosun
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey, Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey, Department of Histology and Embryology, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
  • Pages: 344-348
    |
    Published online on: May 31, 2012
       https://doi.org/10.3892/etm.2012.596
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Abstract

We aimed to determine whether rotenone treatment prevents induced ischemia/reperfusion (I/R) damage in rat bladders by detecting inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) levels by real-time PCR (RT‑PCR). A total of 18 Sprague-Dawley albino rats were used in this experiment. The experimental groups each consisted of 6 rats and were treated as follows: group I, control; group II, I/R; group III, rotenone + I/R. In the control group, the rat bladders were removed by lower abdominal incision without any procedure. In the I/R group, 1 h prior to the ischemia 1 cc physiological serum was administered and the abdominal aortas were clamped for 1 h to achieve bladder ischemia. Following the ischemia, reperfusion was induced for 1 h and the bladders were removed. In the rotenone + I/R group, the rats were treated with 25 mg/kg rotenone intraperitoneally. The iNOS and COX-2 mRNA levels in each group were detected using RT-PCR. In the I/R group, the COX-2 levels in the bladder tissue were higher compared with the control group (P<0.05). The COX-2 levels in the rotenone-treated group were statistically lower compared with the I/R group (P<0.01). Vascularization and edema were markedly increased in the I/R group. Following rotenone treatment these were abrogated inversely to inflammation. Although iNOS levels were slightly higher in the I/R group compared with the control group, iNOS levels did not decrease and no significant difference was observed between the groups with regard to rotenone treatment (P>0.05). We suggest that rotenone may be used clinically to treat I/R damage due to its diminishing effect on COX-2 levels.
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Copy and paste a formatted citation
Spandidos Publications style
Nergiz İ, Başeskioğlu B, Yenilmez A, Erkasap N, Can C and Tosun M: Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model. Exp Ther Med 4: 344-348, 2012.
APA
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., & Tosun, M. (2012). Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model. Experimental and Therapeutic Medicine, 4, 344-348. https://doi.org/10.3892/etm.2012.596
MLA
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., Tosun, M."Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model". Experimental and Therapeutic Medicine 4.2 (2012): 344-348.
Chicago
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., Tosun, M."Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model". Experimental and Therapeutic Medicine 4, no. 2 (2012): 344-348. https://doi.org/10.3892/etm.2012.596
Copy and paste a formatted citation
x
Spandidos Publications style
Nergiz İ, Başeskioğlu B, Yenilmez A, Erkasap N, Can C and Tosun M: Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model. Exp Ther Med 4: 344-348, 2012.
APA
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., & Tosun, M. (2012). Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model. Experimental and Therapeutic Medicine, 4, 344-348. https://doi.org/10.3892/etm.2012.596
MLA
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., Tosun, M."Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model". Experimental and Therapeutic Medicine 4.2 (2012): 344-348.
Chicago
Nergiz, İ., Başeskioğlu, B., Yenilmez, A., Erkasap, N., Can, C., Tosun, M."Effects of rotenone on inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels detected by real-time PCR in a rat bladder ischemia/reperfusion model". Experimental and Therapeutic Medicine 4, no. 2 (2012): 344-348. https://doi.org/10.3892/etm.2012.596
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