Hedyotis diffusa Willd extract inhibits HT‑29 cell proliferation via cell cycle arrest

Lin,Minghe; Lin,Jiumao; Wei,Lihui; Xu,Wei; Hong,Zhenfeng; Cai,Qiaoyan; Peng,Jun; Zhu,Dezeng

doi:10.3892/etm.2012.599

August 2012 Volume 4 Issue 2

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August 2012 Volume 4 Issue 2

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Article

Hedyotis diffusa Willd extract inhibits HT‑29 cell proliferation via cell cycle arrest

Authors:
- Minghe Lin
- Jiumao Lin
- Lihui Wei
- Wei Xu
- Zhenfeng Hong
- Qiaoyan Cai
- Jun Peng
- Dezeng Zhu
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Affiliations: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350108, P.R. China, Department of Pharmacology, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350108, P.R. China
Pages: 307-310
|
Published online on: June 6, 2012

https://doi.org/10.3892/etm.2012.599
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Abstract

Hedyotis diffusa Willd (HDW) has long been used as an important component in several Chinese medicine formulae to clinically treat various types of cancer, including colorectal cancer (CRC). Previously, we reported that HDW inhibits CRC growth via the induction of cancer cell apoptosis and the inhibition of tumor angiogenesis. In the present study, to further elucidate the mechanism of HDW-mediated antitumor activity, we investigated the effect of HDW ethanol extract (EEHDW) on the proliferation of HT-29 human colon carcinoma cells. We found that EEHDW reduced HT-29 cell viability and survival in a dose- and time‑dependent manner. We also observed that EEHDW treatment blocked the cell cycle, preventing G1 to S progression, and reduced mRNA expression of pro-proliferative PCNA, Cyclin D1 and CDK4, but increased that of anti-proliferative p21. Our findings suggest that Hedyotis diffusa Willd may be an effective treatment for CRC via the suppression of cancer cell proliferation.

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1.	Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2001. View Article : Google Scholar : PubMed/NCBI
2.	Nurse P: Ordering S phase and M phase in the cell cycle. Cell. 79:547–550. 1994. View Article : Google Scholar : PubMed/NCBI
3.	Chen Y, Robles AI, Martinez LA, Liu F, Gimenez-Conti IB and Conti CJ: Expression of G1 cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors in androgen-induced prostate proliferation in castrated rats. Cell Growth Differ. 7:1571–1578. 1996.PubMed/NCBI
4.	Graña X and Reddy EP: Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs). Oncogene. 11:211–219. 1995.PubMed/NCBI
5.	Zhong W, Peng J, He H, Wu D, Han Z, Bi X and Dai Q: Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia. Clin Invest Med. 31:E8–E15. 2008.PubMed/NCBI
6.	Harper JW, Adami GR, Wei N, Keyomarsi K and Elledge SJ: The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell. 75:805–816. 1993. View Article : Google Scholar : PubMed/NCBI
7.	Gustin DM and Brenner DE: Chemoprevention of colon cancer: current status and future prospects. Cancer Metast Rev. 21:323–348. 2002. View Article : Google Scholar : PubMed/NCBI
8.	Gorlick R and Bertino JR: Drug resistance in colon cancer. Semin Oncol. 26:606–611. 1999.
9.	Longley DB, Allen WL and Johnston PG: Drug resistance, predictive markers and pharmacogenomics in colorectal cancer. Biochim Biophys Acta. 1766:184–196. 2006.PubMed/NCBI
10.	Boose G and Stopper H: Genotoxicity of several clinically used topoisomerase II inhibitors. Toxicol Lett. 116:7–16. 2000. View Article : Google Scholar : PubMed/NCBI
11.	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar
12.	Kelloff GJ: Perspectives on cancer chemoprevention research and drug development. Adv Cancer Res. 78:199–334. 2000. View Article : Google Scholar : PubMed/NCBI
13.	Tyagi AK, Singh RP and Agarwal C, Chan DC and Agarwal C: Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G2-M arrest, and apoptosis. Clin Cancer Res. 8:3512–3519. 2002.
14.	Newman DJ, Cragg GM and Snader KM: The influence of natural products upon drug discovery. Nat Prod Rep. 17:215–234. 2000. View Article : Google Scholar : PubMed/NCBI
15.	Jeune MA, Kumi-Diaka J and Brown J: Anticancer activities of pomegranate extracts and genistein in human breast cancer cells. J Med Food. 8:469–475. 2005. View Article : Google Scholar : PubMed/NCBI
16.	Sausville EA: Versipelostatin: unfolding an unsweetened death. J Natl Cancer Inst. 96:1266–1267. 2004. View Article : Google Scholar : PubMed/NCBI
17.	Won HJ, Han CH, Kim YH, Kwon HJ, Kim BW, Choi JS and Kim KH: Induction of apoptosis in human acute leukemia Jurkat T cells by Albizzia julibrissin extract is mediated via mitochondria-dependent caspase-3 activation. J Ethnopharmacol. 106:383–389. 2006. View Article : Google Scholar : PubMed/NCBI
18.	Song LR: Zhonghuabencao. 61. Shanghai Science and Technology Press; Shanghai: pp. 4331999
19.	Yang JJ, Hsu HY, Ho YH and Lin CC: Comparative study on the immunocompetent activity of three different kinds of Peh-Hue-Juwa-Chi-Cao, Hedyotis diffusa, H. corymbosa and Mollugo pentaphylla after sublethal whole body X-irradiation. Phytother Res. 11:428–432. 1997. View Article : Google Scholar
20.	Li R, Zhao HR and Lin YM: Anti-tumor effect and protective effect on chemotherapeutic damage of water soluble extracts from Hedyotis diffusa. J Chin Pharm Sci. 11:54–58. 2002.
21.	Lin JM, Chen YQ, Wei LH, Chen XZ, Xu W, Hong ZF, Sferra TJ and Peng J: Hedyotis Diffusa Willd extract induces apoptosis via activation of the mitochondrion-dependent pathway in human colon carcinoma cells. Int J Oncol. 37:1331–1338. 2010.
22.	Lin JM, Wei LH, Xu W, Hong ZF, Liu XX and Peng J: Effect of Hedyotis Diffusa Willd extract on tumor angiogenesis. Mol Med Rep. 4:1283–1288. 2011.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Hedyotis diffusa Willd extract inhibits HT‑29 cell proliferation via cell cycle arrest

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