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Article

Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells

  • Authors:
    • Takashi Masui
    • Ichiro Ota
    • Masatoshi Kanno
    • Katsunari Yane
    • Hiroshi Hosoi
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Nara, Japan, Department of Oncology Center, Nara Medical University, Nara, Japan, Department of Otolaryngology, Kinki University School of Medicine, Nara Hospital, Nara, Japan
  • Pages: 11-16
    |
    Published online on: October 10, 2012
       https://doi.org/10.3892/etm.2012.739
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Abstract

The potential clinical use of ultrasound in inducing cell apoptosis and enhancing the effects of anticancer drugs in the treatment of cancers has previously been investigated. In this study, the combined effects of low-intensity ultrasound (LIU) and cetuximab, an anti-epidermal growth factor receptor (EGFR) antibody, on cell killing and induction of apoptosis in HSC-3 and HSC-4 head and neck cancer cells, and its mechanisms were investigated. Experiments were divided into 4 groups: non-treated (CNTRL), cetuximab-treated (CETU), ultrasound-treated (UST) and the combination of cetuximab and US-treated (COMB). Cell viability was assessed by trypan blue staining assay and induction of apoptosis was detected by fluorescein isothiocyanate (FITC)-Annexin V and propidium iodide (PI) staining assay at 24 h after cetuximab and/or US treatment. To elucidate the effect of cetuximab and US on EGFR signaling and apoptosis in head and neck cancer cells after the treatments, the expression of EGFR, phospho-EGFR, and the activation of caspase-3 were evaluated with western blotting. More cell killing features were evident in the COMB group in HSC-3 and HSC-4 cells compared with the other groups. No differences in EGFR expression among the CETU, UST and COMB groups was observed, while the expression of phospho-EGFR in the CETU group was downregulated compared with that in the CNTRL group. Phospho-EGFR expression was much more downregulated in the COMB group compared with that in the other groups. In addition, the activation of caspase-3 in the UST group was upregulated compared with that in the CNTRL group. Caspase-3 activation was much more upregulated in the COMB group than that in the other groups. These data indicated that LIU was able to enhance the anticancer effect of cetuximab in HSC-3 and HSC-4 head and neck cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Masui T, Ota I, Kanno M, Yane K and Hosoi H: Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells. Exp Ther Med 5: 11-16, 2013.
APA
Masui, T., Ota, I., Kanno, M., Yane, K., & Hosoi, H. (2013). Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells. Experimental and Therapeutic Medicine, 5, 11-16. https://doi.org/10.3892/etm.2012.739
MLA
Masui, T., Ota, I., Kanno, M., Yane, K., Hosoi, H."Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells". Experimental and Therapeutic Medicine 5.1 (2013): 11-16.
Chicago
Masui, T., Ota, I., Kanno, M., Yane, K., Hosoi, H."Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells". Experimental and Therapeutic Medicine 5, no. 1 (2013): 11-16. https://doi.org/10.3892/etm.2012.739
Copy and paste a formatted citation
x
Spandidos Publications style
Masui T, Ota I, Kanno M, Yane K and Hosoi H: Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells. Exp Ther Med 5: 11-16, 2013.
APA
Masui, T., Ota, I., Kanno, M., Yane, K., & Hosoi, H. (2013). Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells. Experimental and Therapeutic Medicine, 5, 11-16. https://doi.org/10.3892/etm.2012.739
MLA
Masui, T., Ota, I., Kanno, M., Yane, K., Hosoi, H."Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells". Experimental and Therapeutic Medicine 5.1 (2013): 11-16.
Chicago
Masui, T., Ota, I., Kanno, M., Yane, K., Hosoi, H."Low-intensity ultrasound enhances the anticancer activity of cetuximab in human head and neck cancer cells". Experimental and Therapeutic Medicine 5, no. 1 (2013): 11-16. https://doi.org/10.3892/etm.2012.739
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