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Article

C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury

  • Authors:
    • Zhangfu Wang
    • Chuanyi Zhang
    • Zhenghua Hong
    • Haixiao Chen
    • Weifu Chen
    • Guofu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Taizhou Hospital of Zhejiang Province, Taizhou, Zhejiang 317000, P.R. China
  • Pages: 107-111
    |
    Published online on: October 12, 2012
       https://doi.org/10.3892/etm.2012.745
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Abstract

Spinal cord injury (SCI) is a severe health problem and the mechanism involved remains elusive. The aim of the present study was to elucidate the role of C/EBP homologous protein (CHOP), a prominent protein of the endoplasmic reticulum (ER) stress-mediated apoptosis in SCI. A total of 20 adult male Sprague-Dawley rats were divided into two groups at random, ten rats were subjected to a modified Allen's test (using a weight-drop device) to induce a SCI model and the remaining ten rats only had the corresponding vertebral lamina removed with no injury and served as the sham-operated group. Pathological changes in the spinal cord were observed 12 h after injury by hematoxylin and eosin staining and TUNEL staining was performed to visualize apoptotic cells. The expression of CHOP was also detected by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction. The results showed that a typical apoptotic morphology, namely the increased the number of TUNEL-positive cells in the injured spinal cord. The expression levels of CHOP in the rats with SCI were increased compared with the sham-operated rats (P<0.05). These results revealed that CHOP-mediated ER stress-induced apoptosis may be involved in SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Zhang C, Hong Z, Chen H, Chen W and Chen G: C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury. Exp Ther Med 5: 107-111, 2013.
APA
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., & Chen, G. (2013). C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury. Experimental and Therapeutic Medicine, 5, 107-111. https://doi.org/10.3892/etm.2012.745
MLA
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., Chen, G."C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury". Experimental and Therapeutic Medicine 5.1 (2013): 107-111.
Chicago
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., Chen, G."C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury". Experimental and Therapeutic Medicine 5, no. 1 (2013): 107-111. https://doi.org/10.3892/etm.2012.745
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Zhang C, Hong Z, Chen H, Chen W and Chen G: C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury. Exp Ther Med 5: 107-111, 2013.
APA
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., & Chen, G. (2013). C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury. Experimental and Therapeutic Medicine, 5, 107-111. https://doi.org/10.3892/etm.2012.745
MLA
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., Chen, G."C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury". Experimental and Therapeutic Medicine 5.1 (2013): 107-111.
Chicago
Wang, Z., Zhang, C., Hong, Z., Chen, H., Chen, W., Chen, G."C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury". Experimental and Therapeutic Medicine 5, no. 1 (2013): 107-111. https://doi.org/10.3892/etm.2012.745
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