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Article

Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice

  • Authors:
    • Jin Seok Kang
    • Hwan-Goo Kang
    • Young-Il Park
    • Hyunjung Lee
    • Kiho Park
    • Yun-Seok Lee
    • Soohee Kim
    • Doug-Young Ryu
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Laboratory Science, Namseoul University, Cheonan 330-707, Republic of Korea, Toxicology Laboratory, Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, MIFAFF, Anyang 480-757, Republic of Korea, Laboratory of Genomics and Quantitative Real-Time PCR, Biomedical Research Institute, Seoul National University Hospital, Seoul 110-744, Republic of Korea , Department of Health Administration, Namseoul University, Cheonan 330-707, Republic of Korea, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea
  • Pages: 138-142
    |
    Published online on: October 17, 2012
       https://doi.org/10.3892/etm.2012.751
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Abstract

To clarify the role of stem cells in hepatocarcinogenesis, the expression of epithelial cell adhesion molecule (EpCAM) and proliferating cell nuclear antigen (PCNA) was investigated in mouse hepatic tumors and embryonic cell lineages. Ten ICR mice were treated with diethylnitrosamine (DEN) at 14 days of age and sacrificed at 36 weeks subsequent to DEN treatment to obtain the hepatic tumors. Mouse embryonic stem cells, hepatic progenitor cells and hepatocyte‑like cells, representing 0, 22 and 40 days of differentiation, respectively, were treated in vitro with DEN at four doses (0, 1, 5 and 15 mM; G1, G2, G3 and G4, respectively) for 24 h and RNA was isolated. A total of 71 hepatic tumors were obtained from the DEN-treated mice. EpCAM expression was increased mainly in hepatic tumor cells, although it was also detected in the surrounding visually normal cells. Double staining showed that EpCAM and PCNA were co-expressed in numerous tumor cells. In vitro, EpCAM expression was significantly different for G4 at day 0 (P<0.01) and for G2, G3 and G4 at day 40 (P<0.01) compared with the control (G1) at the corresponding time-point. PCNA expression was significantly different for G3 and G4 at day 0 (P<0.01), for G2, G3 and G4 at day 22 (P<0.01) and for G2 at day 40 (P<0.01) compared with G1 at the corresponding time-point. In summary, the expression of EpCAM and PCNA was increased in DEN-induced tumors and the expression of EpCAM and PCNA was altered by DEN treatment in cultured cells. This suggests that EpCAM expression may be modulated in the progeny of adult liver stem cells during their differentiation toward hepatocytes and may be increased during DEN-induced hepatocarcinogenesis.
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1 

Rambhatla L, Chiu CP, Kundu P, Peng Y and Carpenter MK: Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant. 12:1–11. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Turner R, Lozoya O, Wang Y, Cardinale V, Gaudio E, Alpini G, Mendel G, Wauthier E, Barbier C, Alvaro D and Reid LM: Human hepatic stem cell and maturational liver lineage biology. Hepatology. 53:1035–1045. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kenyon J and Gerson SL: The role of DNA damage repair in aging of adult stem cells. Nucleic Acids Res. 35:7557–7565. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Alison MR: Liver stem cells: implications for hepatocarcinogenesis. Stem Cell Rev. 1:253–260. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Sell S and Dunsford HA: Evidence for the stem cell origin of hepatocellular carcinoma and cholangiocarcinoma. Am J Pathol. 134:1347–1363. 1989.PubMed/NCBI

6 

Visvader JE and Lindeman GJ: Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat Rev Cancer. 8:755–768. 2008. View Article : Google Scholar : PubMed/NCBI

7 

van der Gun BT, Melchers LJ, Ruiters MH, de Leij LF, McLaughlin PM and Rots MG: EpCAM in carcinogenesis: the good, the bad or the ugly. Carcinogenesis. 31:1913–1921. 2010.PubMed/NCBI

8 

Yoon SM, Gerasimidou D, Kuwahara R, Hytiroglou P, Yoo JE, Park YN and Theise ND: Epithelial cell adhesion molecule (EpCAM) marks hepatocytes newly derived from stem/progenitor cells in humans. Hepatology. 53:964–973. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Yamashita T, Forgues M, Wang W, Kim JW, Ye Q, Jia H, Budhu A, Zanetti KA, Chen Y, Qin LX, Tang ZY and Wang XW: EpCAM and alpha-fetoprotein expression defines novel prognostic subtypes of hepatocellular carcinoma. Cancer Res. 68:1451–1461. 2008. View Article : Google Scholar

10 

Fausto N and Campbell JS: Mouse models of hepatocellular carcinoma. Semin Liver Dis. 30:87–98. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Diwan BA, Rice JM, Ward JM, Ohshima M and Lynch PH: Inhibition by phenobarbital and lack of effect of amobarbital on the development of liver tumors induced by N-nitrosodiethylamine in juvenile B6C3F1 mice. Cancer Lett. 23:223–234. 1984. View Article : Google Scholar : PubMed/NCBI

12 

Jang JJ, Weghorst CM, Henneman JR, Devor DE and Ward JM: Progressive atypia in spontaneous and N-nitrosodiethylamine-induced hepatocellular adenomas of C3H/HeNCr mice. Carcinogenesis. 13:1541–1547. 1992. View Article : Google Scholar : PubMed/NCBI

13 

Tamano S, Merlino GT and Ward JM: Rapid development of hepatic tumors in transforming growth factor alpha transgenic mice associated with increased cell proliferation in precancerous hepatocellular lesions initiated by N-nitrosodiethylamine and promoted by phenobarbital. Carcinogenesis. 15:1791–1798. 1994. View Article : Google Scholar

14 

Jensen MR, Factor VM, Fantozzi A, Helin K, Huh CG and Thorgeirsson SS: Reduced hepatic tumor incidence in cyclin G1-deficient mice. Hepatology. 37:862–870. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Vesselinovitch SD, Koka M, Mihailovich N and Rao KV: Carcinogenicity of diethylnitrosamine in newborn, infant, and adult mice. J Cancer Res Clin Oncol. 108:60–65. 1984. View Article : Google Scholar : PubMed/NCBI

16 

Zhou QJ, Xiang LX, Shao JZ, Hu RZ, Lu YL, Yao H and Dai LC: In vitro differentiation of hepatic progenitor cells from mouse embryonic stem cells induced by sodium butyrate. J Cell Biochem. 100:29–42. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Yovchev MI, Grozdanov PN, Zhou H, Racherla H, Guha C and Dabeva MD: Identification of adult hepatic progenitor cells capable of repopulating injured rat liver. Hepatology. 47:636–647. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Hamazaki T, Iiboshi Y, Oka M, Papst PJ, Meacham AM, Zon LI and Terada N: Hepatic maturation in differentiating embryonic stem cells in vitro. FEBS Lett. 497:15–19. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Touboul T, Hannan NR, Corbineau S, Martinez A, Martinet C, Branchereau S, Mainot S, Strick-Marchand H, Pedersen R, Di Santo J, Weber A and Vallier L: Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology. 51:1754–1765. 2010. View Article : Google Scholar

20 

Wicha MS, Liu S and Dontu G: Cancer stem cells: an old idea - a paradigm shift. Cancer Res. 66:1883–1890; discussion 1895–1886, 2006.

21 

Al-Hajj M and Clarke MF: Self-renewal and solid tumor stem cells. Oncogene. 23:7274–7282. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Kang JS, Wanibuchi H, Morimura K, Gonzalez FJ and Fukushima S: Role of CYP2E1 in diethylnitrosamine-induced hepatocarcinogenesis in vivo. Cancer Res. 67:11141–11146. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Gray R, Peto R, Brantom P and Grasso P: Chronic nitrosamine ingestion in 1040 rodents: the effect of the choice of nitrosamine, the species studied, and the age of starting exposure. Cancer Res. 51:6470–6491. 1991.PubMed/NCBI

24 

Pitot HC, Dragan YP, Teeguarden J, Hsia S and Campbell H: Quantitation of multistage carcinogenesis in rat liver. Toxicol Pathol. 24:119–128. 1996. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kang JS, Kang H, Park Y, Lee H, Park K, Lee Y, Kim S and Ryu D: Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice. Exp Ther Med 5: 138-142, 2013.
APA
Kang, J.S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y. ... Ryu, D. (2013). Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice. Experimental and Therapeutic Medicine, 5, 138-142. https://doi.org/10.3892/etm.2012.751
MLA
Kang, J. S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y., Kim, S., Ryu, D."Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice". Experimental and Therapeutic Medicine 5.1 (2013): 138-142.
Chicago
Kang, J. S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y., Kim, S., Ryu, D."Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice". Experimental and Therapeutic Medicine 5, no. 1 (2013): 138-142. https://doi.org/10.3892/etm.2012.751
Copy and paste a formatted citation
x
Spandidos Publications style
Kang JS, Kang H, Park Y, Lee H, Park K, Lee Y, Kim S and Ryu D: Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice. Exp Ther Med 5: 138-142, 2013.
APA
Kang, J.S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y. ... Ryu, D. (2013). Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice. Experimental and Therapeutic Medicine, 5, 138-142. https://doi.org/10.3892/etm.2012.751
MLA
Kang, J. S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y., Kim, S., Ryu, D."Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice". Experimental and Therapeutic Medicine 5.1 (2013): 138-142.
Chicago
Kang, J. S., Kang, H., Park, Y., Lee, H., Park, K., Lee, Y., Kim, S., Ryu, D."Expression of epithelial cell adhesion molecule and proliferating cell nuclear antigen in diethylnitrosamine‑induced hepatocarcinogenesis in mice". Experimental and Therapeutic Medicine 5, no. 1 (2013): 138-142. https://doi.org/10.3892/etm.2012.751
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