Pathogenic analysis of sputum from ventilator-associated pneumonia in a pediatric intensive care unit

Ning,Bo-Tao; Zhang,Chen-Mei; Liu,Tao; Ye,Sheng; Yang,Zi-Hao; Chen,Zhen-Jie

doi:10.3892/etm.2012.757

January 2013 Volume 5 Issue 1

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January 2013 Volume 5 Issue 1

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Article

Pathogenic analysis of sputum from ventilator-associated pneumonia in a pediatric intensive care unit

Authors:
- Bo-Tao Ning
- Chen-Mei Zhang
- Tao Liu
- Sheng Ye
- Zi-Hao Yang
- Zhen-Jie Chen
View Affiliations / Copyright

Affiliations: Department of Pediatric Intensive Care Unit, Children's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Pages: 367-371
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Published online on: October 22, 2012

https://doi.org/10.3892/etm.2012.757
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Abstract

Ventilator-associated pneumonia (VAP) is a common and sometimes fatal complication in pediatric intensive care units (PICU). The aim of our study was to characterize the distribution and drug susceptibility of the pathogens isolated from the sputum of patients with VAP in the PICU of our hospital and to provide support to the administration of antibiotics early and reasonably in the clinic. Our study was conducted between January 2007 and December 2011 at the PICU of the Children's Hospital of Zhejiang University School of Medicine. The endotracheal aspirates were collected and transported to a microbiology laboratory within 15 min. The pathogens were routinely analyzed and identified with Vitek 60 and Kirby-Bauer disk diffusion methods. Among the 121 VAP patients, 127 pathogenic strains were isolated from sputum specimens. Gram-negative and gram-positive bacteria and fungi accounted for 64.57% (82/127), 29.92% (38/127) and 5.51% (7/127), respectively. Acinetobacter baumannii (25.61%), Escherichia coli (20.27%), Stenotrophomonas maltophilia (20.27%), Klebsiella pneumoniae (16.22%) and Pseudomonas aeruginosa (9.46%) were frequently identified isolates among gram-negative bacteria. Staphylococci were susceptible to vancomycin and linezolid. All fungi were sensitive to the antimicrobial agents. The gram-negative bacteria were more prevalent than gram-positive bacteria and fungi in VAP and demonstrated a higher drug resistance. It is important to administer antimicrobial agents early and reasonably for children with VAP. Knowledge of antibiotic resistance and the characteristics of drug resistance is important for VAP prophylaxis and treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Pathogenic analysis of sputum from ventilator-associated pneumonia in a pediatric intensive care unit

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