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Article

Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus

  • Authors:
    • Si-Feng Shi
    • Xian-Long Zhang
    • Chen Zhu
    • De-Sheng Chen
    • Yong-Yuan Guo
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, P.R. China
  • Pages: 257-262
    |
    Published online on: October 22, 2012
       https://doi.org/10.3892/etm.2012.758
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Abstract

Staphylococcus aureus (S. aureus) is the principle causative agent of osteomyelitis, accounting for 80% of all human cases. S. aureus internalized in osteoblasts escapes immune response, including engulfment by phagocytes. It also escapes the action of a number of antibiotics. Ultrasound increases cell membrane permeability to a number of drugs. Following an internalization assay, we used low-frequency, low-power ultrasound combined with the antibiotic rifampin to target S. aureus internalized in human osteoblasts. Tryptic soy agar (TSA) was used to quantitate the antibacterial effect of rifampin combined with low-frequency ultrasound. A Cell Counting Kit-8 (CCK-8) assay was used to evaluate cell viability following exposure to ultrasound. Our data revealed that rifampin successfully penetrates into osteoblasts and kills internalized S. aureus in osteoblasts, while low-frequency ultrasound promotes this process. Ultrasound had a negative impact on the cell viability of osteoblasts; however, this damage was slight and reversible. Ultrasound-enhanced antibiotic efficiency to bacteria internalized in the osteoblasts may contribute to the control of chronic infection to reduce recurrence.
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Copy and paste a formatted citation
Spandidos Publications style
Shi S, Zhang X, Zhu C, Chen D and Guo Y: Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus. Exp Ther Med 5: 257-262, 2013.
APA
Shi, S., Zhang, X., Zhu, C., Chen, D., & Guo, Y. (2013). Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus. Experimental and Therapeutic Medicine, 5, 257-262. https://doi.org/10.3892/etm.2012.758
MLA
Shi, S., Zhang, X., Zhu, C., Chen, D., Guo, Y."Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus". Experimental and Therapeutic Medicine 5.1 (2013): 257-262.
Chicago
Shi, S., Zhang, X., Zhu, C., Chen, D., Guo, Y."Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus". Experimental and Therapeutic Medicine 5, no. 1 (2013): 257-262. https://doi.org/10.3892/etm.2012.758
Copy and paste a formatted citation
x
Spandidos Publications style
Shi S, Zhang X, Zhu C, Chen D and Guo Y: Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus. Exp Ther Med 5: 257-262, 2013.
APA
Shi, S., Zhang, X., Zhu, C., Chen, D., & Guo, Y. (2013). Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus. Experimental and Therapeutic Medicine, 5, 257-262. https://doi.org/10.3892/etm.2012.758
MLA
Shi, S., Zhang, X., Zhu, C., Chen, D., Guo, Y."Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus". Experimental and Therapeutic Medicine 5.1 (2013): 257-262.
Chicago
Shi, S., Zhang, X., Zhu, C., Chen, D., Guo, Y."Ultrasonically enhanced rifampin activity against internalized Staphylococcus aureus". Experimental and Therapeutic Medicine 5, no. 1 (2013): 257-262. https://doi.org/10.3892/etm.2012.758
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