Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions

Zhu,Shaobo; Zhang,Tao; Sun,Chen; Yu,Aixi; Qi,Baiwen; Cheng,Hao

doi:10.3892/etm.2012.765

January 2013 Volume 5 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January 2013 Volume 5 Issue 1

Full Size Image

Article

Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions

Authors:
- Shaobo Zhu
- Tao Zhang
- Chen Sun
- Aixi Yu
- Baiwen Qi
- Hao Cheng
View Affiliations / Copyright

Affiliations: Department of Micro-Orthopedics, Zhongnan Hospital, Wuhan University, Wuhan 430071, P.R. China
Pages: 95-101
|
Published online on: October 25, 2012

https://doi.org/10.3892/etm.2012.765
Expand metrics +

Abstract

The aim of this study was to investigate the feasibility of Ad-hTGF-β1-transfected bone marrow mesenchymal stem cells (BMMSCs) combined with calcium alginate gel for the construction of tissue-engineered cartilage under three-dimensional conditions. Rat BMMSCs were divided into three groups: the Ad-hTGF-β1 transfection group, the Ad-EGFP transfection group and the control group. The BMMSCs in the Ad-hTGF-β1 transfection group were continually grown. The compound of cell-calcium alginate gel was cultured in a constant temperature incubator. The morphology of cells was examined, and the proliferation of cells was detected by MTT assay. The results from real-time PCR showed that the average relative ratio of TGF-β1 and transcriptional coactivator with PDZ-binding motif (TAZ) in the Ad-hTGF-β1 group was comparable to that of the control group (P<0.05). Using western blotting and immunohistochemistry, strong expression of collagen II in the Ad-hTGF-β1 group was detected. The results from western blotting showed that the expression of TGF-β1 in the Ad-hTGF-β1 group was significantly increased compared with that of the other two groups. The differentiation of BMMSCs was induced by Ad-hTGF-β1 transfection into chondrocytes. TGF-β1 may promote the differentiation of BMMSCs into chondrocytes by TAZ. BMMSCs transfected by Ad-hTGF-β1 could be induced into chondrocytes. These three-dimensional conditions could preferably mimic cell growth patterns in vivo.

View Figures

View References

1	Ghidoni I, Chlapanidas T, Bucco M, et al: Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine. Cytotechnology. 58:49–56. 2008. View Article : Google Scholar : PubMed/NCBI
2	Lin YJ, Yen CN, Hu YC, et al: Chondrocytes culture in three-dimensional porous alginate scaffolds enhanced cell proliferation, matrix synthesis and gene expression. J Biomed Mater Res A. 88:23–33. 2009. View Article : Google Scholar : PubMed/NCBI
3	Kanda C, Barry WO and Marie-Paule I: Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels. J Orthop Res. 20:1070–1078. 2002. View Article : Google Scholar : PubMed/NCBI
4	Yao Xiawan, Wei Liu, Tianyi Liu, et al: Application of bone marrow stromal cells in vitro tubular cartilage tissue engineering experiments. Chin J Microsurg. 28:328–330. 2005.
5	Jian Zhao and Wei Xu: Bone marrow mesenchymal stem cells and embryonic stem cells into cartilage cells into possible molecular mechanisms [J]. Tissue Eng Reconstructive Surg. 2:53–57. 2006.PubMed/NCBI
6	Hong JH, Hwang ES, McManus MT, et al: TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science. 309:1074–1078. 2005. View Article : Google Scholar : PubMed/NCBI
7	Zhao L, Jiang S and Hantash BM: TGF-beta1 induces osteogenic differentiation of murine bone marrow stromal cells. Tissue Eng Part A. 16:725–733. 2010. View Article : Google Scholar : PubMed/NCBI
8	Hong JH and Yaffe MB: TAZ: a beta-catenin-like molecule that regulates mesenchymal stem cell differentiation. Cell Cycle. 5:176–179. 2006. View Article : Google Scholar : PubMed/NCBI
9	Lund AW, Yener B, Stegemann JP and Plopper GE: The natural and engineered 3D microenvironment as a regulatory cue during stem cell fate determination. Tissue Eng Part B Rev. 15:371–380. 2009. View Article : Google Scholar : PubMed/NCBI
10	Purpura KA, Aubin JE and Zandstra PW: Sustained in vitro expansion of bone progenitors is cell density dependent. Stem Cells. 22:39–50. 2004. View Article : Google Scholar : PubMed/NCBI
11	McBeath R, Pirone DM, Nelson CM, et al: Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell. 6:483–495. 2004. View Article : Google Scholar : PubMed/NCBI
12	Liu WF and Chen CS: Cellular and multicellular form and function. Adv Drug Deliv Rev. 59:1319–1328. 2007. View Article : Google Scholar : PubMed/NCBI
13	Burg KJ, Porter S and Kellam JF: Biomaterial developments for bone tissue engineering. Biomaterials. 21:2347–2359. 2000. View Article : Google Scholar : PubMed/NCBI
14	Benya PD and Shaffer JD: Dediferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell. 30:215–224. 1982. View Article : Google Scholar : PubMed/NCBI
15	Vinatier C, Guicheux J, Daculsi G, et al: Cartilage and bone tissue engineering using hydrogels. Biomed Mater Eng. 16:S107–S113. 2006.PubMed/NCBI
16	Dobratz EJ, Kim SW, Voglewede A and Park SS: Injectable cartilage: using alginate and human chondrocytes. Arch Facial Plast Surg. 11:40–47. 2009. View Article : Google Scholar : PubMed/NCBI
17	Chang SC, Rowley JA, Tobias G, et al: Injection molding of chondrocyte / alginate constructs in the shape of facial implants. J Biomed Mater Res. 55:503–511. 2001. View Article : Google Scholar : PubMed/NCBI
18	Chang SC, Tobias G, Roy AK, Vacanti CA and Bonassar LJ: Tissue engineering of autologous cartilage for craniofacial reconstruction by injection molding. Plast Reconstr Surg. 112:793–799. 2003. View Article : Google Scholar : PubMed/NCBI
19	Yi Guoquan and Shibi Lu: Alginate properties and applications in cartilage tissue engineering. Chin J Orthopaedic Surg. 14:216–218. 2006.
20	Kamishina H, Miyabayashi T, Clemmons RM, et al: Three-dimensional culture of feline articular chondrocytes in alginate microspheres. J Vet Med Sci. 68:1239–1242. 2006. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Related Articles