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Article

Expression of intestinal transporter genes in beagle dogs

  • Authors:
    • Soo‑Min Cho
    • Sung‑Won Park
    • Na‑Hyun Kim
    • Jin‑A Park
    • Hee Yi
    • Hee‑Jung Cho
    • Ki‑Hwan Park
    • Ingyun Hwang
    • Ho‑Chul Shin
  • View Affiliations / Copyright

    Affiliations: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143‑701, Republic of Korea, Toxicology and Chemistry Division, Animal Plant and Fisheries Quarantine and Inspection Agency, Anyang 430‑824, Republic of Korea, Department of Food Science __AMB__ Technology, Chung‑Ang University, Ansung 456‑756, Republic of Korea, Food Chemical Residues Division, Korea Food __AMB__ Drug Administration, Osong 363‑951, Republic of Korea
  • Pages: 308-314
    |
    Published online on: October 30, 2012
       https://doi.org/10.3892/etm.2012.777
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Abstract

This study was performed to produce a transcriptional database of the intestinal transporters of beagle dogs. Total RNA was isolated from the duodenum and the expression of various mRNAs was measured using GeneChip® oligonucleotide arrays. A total of 124 transporter genes were detected. Genes for fatty acid, peptide, amino acid and glucose and multidrug resistance/multidrug resistance‑associated protein (MDR/MRP) transport were expressed at relatively higher levels than the other transporter types. The dogs exhibited abundant mRNA expression of the fatty acid transporters (fatty acid binding proteins, FABPs) FABP1 and FABP2, the ATP‑binding cassettes (ABCs) ABCB1A and ABCC2, the amino acid/peptide transporters SLC3A1 and SLC15A1, the glucose transporters SLC5A1, SLC2A2 and SLC2A5, the organic anion transporter SLC22A9 and the phosphate transporters SLC20A1 and SLC37A4. In mice, a similar profile was observed with high expression of the glucose transporters SLC5A1 and SLC2As, the fatty acid transporters FABP1 and FABP2, the MDR/MRP transporters ABCB1A and ABCC2 and the phosphate transporter SLC37A4. However, the overall data reveal diverse transcriptomic profiles of the intestinal transporters of dogs and mice. Therefore, the current database may be useful for comparing the intestinal transport systems of dogs with those of mice to better evaluate xenobiotics.
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Copy and paste a formatted citation
Spandidos Publications style
Cho SM, Park SW, Kim NH, Park JA, Yi H, Cho HJ, Park KH, Hwang I and Shin HC: Expression of intestinal transporter genes in beagle dogs. Exp Ther Med 5: 308-314, 2013.
APA
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H. ... Shin, H. (2013). Expression of intestinal transporter genes in beagle dogs. Experimental and Therapeutic Medicine, 5, 308-314. https://doi.org/10.3892/etm.2012.777
MLA
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H., Park, K., Hwang, I., Shin, H."Expression of intestinal transporter genes in beagle dogs". Experimental and Therapeutic Medicine 5.1 (2013): 308-314.
Chicago
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H., Park, K., Hwang, I., Shin, H."Expression of intestinal transporter genes in beagle dogs". Experimental and Therapeutic Medicine 5, no. 1 (2013): 308-314. https://doi.org/10.3892/etm.2012.777
Copy and paste a formatted citation
x
Spandidos Publications style
Cho SM, Park SW, Kim NH, Park JA, Yi H, Cho HJ, Park KH, Hwang I and Shin HC: Expression of intestinal transporter genes in beagle dogs. Exp Ther Med 5: 308-314, 2013.
APA
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H. ... Shin, H. (2013). Expression of intestinal transporter genes in beagle dogs. Experimental and Therapeutic Medicine, 5, 308-314. https://doi.org/10.3892/etm.2012.777
MLA
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H., Park, K., Hwang, I., Shin, H."Expression of intestinal transporter genes in beagle dogs". Experimental and Therapeutic Medicine 5.1 (2013): 308-314.
Chicago
Cho, S., Park, S., Kim, N., Park, J., Yi, H., Cho, H., Park, K., Hwang, I., Shin, H."Expression of intestinal transporter genes in beagle dogs". Experimental and Therapeutic Medicine 5, no. 1 (2013): 308-314. https://doi.org/10.3892/etm.2012.777
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