Increased expression of TNF ligand‑related molecule 1A and death receptor 3 in bladder tissues of patients with painful bladder syndrome/interstitial cystitis

  • Authors:
    • Erwei Zhang
    • Xuhui Zhu
    • Song Han
    • Zhifeng Peng
    • Wei Wang
    • Junfa Li
    • Yong Yang
  • View Affiliations

  • Published online on: October 30, 2012     https://doi.org/10.3892/etm.2012.778
  • Pages: 282-286
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Abstract

Members of the tumor necrosis factor (TNF) superfamily have been revealed to be associated with painful bladder syndrome/interstitial cystitis (PBS/IC). TNF ligand‑related molecule 1A (TL1A) and its receptor, death receptor 3 (DR3), belong to the TNF superfamily and have been implicated in chronic inflammatory diseases. Bladder biopsies from 8 female patients clinically diagnosed with PBS/IC according to the National Institute for Diabetes and Digestive and Kidney Diseases criteria and 8 female bladder carcinoma control patients were investigated to test the protein and mRNA expression levels of TL1A and DR3 using western blotting and real‑time RT‑PCR. The protein level ratio of TL1A to β‑actin (IC, 0.65±0.03 vs. controls, 0.25±0.02, P<0.001) and of its receptor DR3 to β‑actin (IC, 0.66±0.06 vs. controls, 0.27±0.02, P<0.001) were observed to be significantly higher in the patients with IC. The real‑time RT‑PCR ∆Cts of TL1A minus GAPDH (IC, 7.60±0.52 vs. controls, 10.08±0.32, P<0.001) and the DR3 minus GAPDH (IC, 6.68±0.60 vs. controls, 8.99±0.61, P=0.017) were observed to be significantly lower in the patients with IC, suggesting that the mRNA levels of TL1A and DR3 were higher in the PBS/IC patients. The protein and mRNA expression of TL1A and DR3 are upregulated in the bladder tissues of PBS/IC patients and may be involved in inflammation and apoptosis in PBS/IC.
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January 2013
Volume 5 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang E, Zhu X, Han S, Peng Z, Wang W, Li J and Yang Y: Increased expression of TNF ligand‑related molecule 1A and death receptor 3 in bladder tissues of patients with painful bladder syndrome/interstitial cystitis. Exp Ther Med 5: 282-286, 2013.
APA
Zhang, E., Zhu, X., Han, S., Peng, Z., Wang, W., Li, J., & Yang, Y. (2013). Increased expression of TNF ligand‑related molecule 1A and death receptor 3 in bladder tissues of patients with painful bladder syndrome/interstitial cystitis. Experimental and Therapeutic Medicine, 5, 282-286. https://doi.org/10.3892/etm.2012.778
MLA
Zhang, E., Zhu, X., Han, S., Peng, Z., Wang, W., Li, J., Yang, Y."Increased expression of TNF ligand‑related molecule 1A and death receptor 3 in bladder tissues of patients with painful bladder syndrome/interstitial cystitis". Experimental and Therapeutic Medicine 5.1 (2013): 282-286.
Chicago
Zhang, E., Zhu, X., Han, S., Peng, Z., Wang, W., Li, J., Yang, Y."Increased expression of TNF ligand‑related molecule 1A and death receptor 3 in bladder tissues of patients with painful bladder syndrome/interstitial cystitis". Experimental and Therapeutic Medicine 5, no. 1 (2013): 282-286. https://doi.org/10.3892/etm.2012.778