Combined analysis of serum γ-glutamyl transferase isoenzyme II, α-L-fucosidase and α-fetoprotein detected using a commercial kit in the diagnosis of hepatocellular carcinoma

Zhu,Jing; Jiang,Feng; Ni,Hong-Bing; Xiao,Ming-Bing; Chen,Bu-You; Ni,Wen-Kai; Lu,Cui-Hua; Ni,Run-Zhou

doi:10.3892/etm.2012.783

January 2013 Volume 5 Issue 1

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January 2013 Volume 5 Issue 1

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Article

Combined analysis of serum γ-glutamyl transferase isoenzyme II, α-L-fucosidase and α-fetoprotein detected using a commercial kit in the diagnosis of hepatocellular carcinoma

Authors:
- Jing Zhu
- Feng Jiang
- Hong-Bing Ni
- Ming-Bing Xiao
- Bu-You Chen
- Wen-Kai Ni
- Cui-Hua Lu
- Run-Zhou Ni
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Medical Laboratory Center, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Radiochemotherapy, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Pages: 89-94
|
Published online on: October 31, 2012

https://doi.org/10.3892/etm.2012.783
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Abstract

γ‑glutamyl transferase isoenzyme II (GGT‑II) is a sensitive biomarker of hepatocellular carcinoma (HCC). However, numerous disadvantages of the traditional manual method affected its application. The commercial kit provided a convenient and fast method for the determination of GGT‑II levels. The purposes of the present study were to compare the reproducibility and sensitivity between the manual and commercial kit methods and to evaluate the diagnostic efficiency for HCC with the combined analysis of GGT‑II, α‑L‑fucosidase (AFU) and α‑fetoprotein (AFP). In patients with various liver diseases (HCC, liver cirrhosis and chronic hepatitis) and normal subjects, GGT‑II was detected by manual and commercial polyacrylamide gel electrophoresis (PAGE). The levels of AFU and AFP were assayed by colorimetry and a chemiluminescence immunoassay, respectively. The commercial PAGE had equal diagnostic efficiency with traditional manual PAGE and no significant differences were observed in intra‑ and average‑gel reproducibility and GGT‑II sensitivities between the manual and commercial PAGE (P>0.05). The incidence of GGT‑II detected by commercial PAGE in HCC patients was 84.1% and <8% in benign liver disease. The levels of AFU and AFP in the benign liver diseases and normal subjects were lower than those in HCC. According to the cut‑off value obtained by receiver operating characteristic curves, a total of 56.6 and 59.3% of HCC patients (64 out of 113 and 67 out of 113) had AFU >636.5 µmol/l h and AFP >44.0 µg/l, respectively. There were no significant correlations between GGT‑II and AFU or AFP. Combined detection of GGT‑II with AFU or AFP increased the diagnostic sensitivity to 92.9 and 93.8%, respectively. These results suggest that commercial PAGE provides a simple and reproducible method for GGT‑II detection. Combined determination of GGT‑II with AFU or AFP exhibited superior sensitivity and specificity for the diagnosis of HCC.

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1	Chiappini F: Circulating tumor cells measurements in hepatocellular carcinoma. Int J Hepatol. 2012:6848022012. View Article : Google Scholar : PubMed/NCBI
2	El-Serag HB: Hepatocellular carcinoma. N Engl J Med. 365:1118–1127. 2011. View Article : Google Scholar : PubMed/NCBI
3	Hiotis SP, Rahbari NN, Villanueva GA, Klegar E, Luan W, Wang Q and Yee HT: Hepatitis B vs. hepatitis C infection on viral hepatitis-associated hepatocellular carcinoma. BMC Gastroenterol. 12:642012. View Article : Google Scholar : PubMed/NCBI
4	Tang ZY: Hepatocellular carcinoma - cause, treatment and metastasis. World J Gastroenterol. 7:445–454. 2001.PubMed/NCBI
5	Yao DF, Dong ZZ and Yao M: Specific molecular markers in hepatocellular carcinoma. Hepatobiliary Pancreat Dis Int. 6:241–247. 2007.PubMed/NCBI
6	Malaguarnera G, Giordano M, Paladina I, Berretta M, Cappellani A and Malaguarnera M: Serum markers of hepatocellular carcinoma. Dig Dis Sci. 55:2744–2755. 2010. View Article : Google Scholar : PubMed/NCBI
7	El-Tayeh SF, Hussein TD, El-Houseini ME, Amer MA, El-Sherbini M and Elshemey WM: Serological biomarkers of hepatocellular carcinoma in Egyptian patients. Dis Markers. 32:255–263. 2012. View Article : Google Scholar : PubMed/NCBI
8	Bertino G, Ardiri A, Malaguarnera M, Malaguarnera G, Bertino N and Calvagno GS: Hepatocellualar carcinoma serum markers. Semin Oncol. 39:410–433. 2012. View Article : Google Scholar : PubMed/NCBI
9	Yao DF, Dong ZZ, Yao DB, Wu XH, Wu W, Qiu LW, Wang HM and Meng XY: Abnormal expression of hepatoma-derived gamma-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes. Hepatobiliary Pancreat Dis Int. 3:564–570. 2004.PubMed/NCBI
10	Okuyama K: Separation and identification of serum gamma-glutamyl transpeptidase isoenzymes by wheat germ agglutinin affinity electrophoresis: a basic analysis and its clinical application to various liver diseases. Keio J Med. 42:149–156. 1993.
11	Bellin M, Tumino E, Giordani R, Fabrini G, Costa F, Galli R, et al: Serum gamma-glutamyl transpeptidase isoforms in alcoholic liver disease. Alcohol Alcohol. 32:259–266. 1997. View Article : Google Scholar : PubMed/NCBI
12	Xu K, Meng XY, Wu JW, Shen B, Shi YC and Wei Q: Diagnostic value of serum gamma-glutamyl transferase isoenzyme for hepatocellular carcinoma: a 10-year study. Am J Gastroenterol. 87:991–995. 1992.PubMed/NCBI
13	Xu KC, Meng XY, Shi YC, Ge ZJ, Ye L, Yu ZJ and Yang DM: The diagnostic value of a hepatoma-specific band of serum gamma-glutamyl transferase. Int J Cancer. 36:667–669. 1985. View Article : Google Scholar : PubMed/NCBI
14	Whitfield JB: Gamma glutamyl transferase. Crit Rev Clin Lab Sci. 38:263–355. 2001. View Article : Google Scholar
15	Ikeda Y and Taniguchi N: Gene expression of gamma-glutamyltranspeptidase. Methods Enzymol. 401:408–425. 2005. View Article : Google Scholar : PubMed/NCBI
16	Kojima J, Kanatani M, Nakamura N, Kashiwagi T, Tohjoh F and Akiyama M: Electrophoretic fractionation of serum gamma-glutamyl transpeptidase in human hepatic cancer. Clin Chim Acta. 106:165–172. 1980. View Article : Google Scholar : PubMed/NCBI
17	Sawabu N, Nakagen M, Ozaki K, Wakabayashi T, Toya D, Hattori N and Ishii M: Clinical evaluation of specific gamma-GTP isoenzyme in patients with hepatocellular carcinoma. Cancer. 51:327–331. 1983. View Article : Google Scholar : PubMed/NCBI
18	Kew MC, Wolf P, Whittaker D and Rowe P: Tumour-associated isoenzymes of gamma-glutamyl transferase in the serum of patients with hepatocellular carcinoma. Br J Cancer. 50:451–455. 1984. View Article : Google Scholar : PubMed/NCBI
19	Cui R, He J, Zhang F, Wang B, Ding H, Shen H, Li Y and Chen X: Diagnostic value of protein induced by vitamin K absence (PIVKAII) and hepatoma-specific band of serum gamma-glutamyl transferase (GGTII) as hepatocellular carcinoma markers complementary to alpha-fetoprotein. Br J Cancer. 88:1878–1882. 2003. View Article : Google Scholar
20	Yao DF and Dong ZZ: Hepatoma-related gamma-glutamyl transferase in laboratory or clinical diagnosis of hepatocellular carcinoma. Hepatobiliary Pancreat Dis Int. 6:9–11. 2007.PubMed/NCBI
21	Grizzi F, Franceschini B, Hamrick C, Frezza EE, Cobos E and Chiriva-Internati M: Usefulness of cancer-testis antigens as biomarkers for the diagnosis and treatment of hepatocellular carcinoma. J Transl Med. 5:32007. View Article : Google Scholar : PubMed/NCBI
22	Tangkijvanich P, Tosukhowong P, Bunyongyod P, Lertmaharit S, Hanvivatvong O, Kullavanijaya P and Poovorawan Y: Alpha-L-fucosidase as a serum marker of hepatocellular carcinoma in Thailand. Southeast Asian J Trop Med Public Health. 30:110–114. 1999.PubMed/NCBI
23	el-Houseini ME, Mohammed MS, Elshemey WM, Hussein TD, Desouky OS and Elsayed AA: Enhanced detection of hepatocellular carcinoma. Cancer Control. 12:248–253. 2005.PubMed/NCBI
24	Wang JJ and Cao EH: Rapid kinetic rate assay of the serum alpha-L-fucosidase in patients with hepatocellular carcinoma by using a novel substrate. Clin Chim Acta. 347:103–109. 2004. View Article : Google Scholar : PubMed/NCBI
25	Zhou L, Liu J and Luo F: Serum tumor markers for detection of hepatocellular carcinoma. World J Gastroenterol. 12:1175–1181. 2006.PubMed/NCBI
26	Gomaa AI, Khan SA, Leen EL, Waked I and Taylor-Robinson SD: Diagnosis of hepatocellular carcinoma. World J Gastroenterol. 15:1301–1314. 2009. View Article : Google Scholar : PubMed/NCBI
27	Wright LM, Kreikemeier JT and Fimmel CJ: A concise review of serum markers for hepatocellular cancer. Cancer Detect Prev. 31:35–44. 2007. View Article : Google Scholar : PubMed/NCBI