Expression of Das-1, Ki67 and sulfuric proteins in gastric cardia adenocarcinoma and intestinal metaplasia lesions

Feng,Xiao-Shan; Wang,Yu-Feng; Hao,Shu-Guang; Ru,Yi; Gao,She-Gan; Wang,Li-Dong

doi:10.3892/etm.2013.1038

Expression of Das-1, Ki67 and sulfuric proteins in gastric cardia adenocarcinoma and intestinal metaplasia lesions

Authors:
- Xiao-Shan Feng
- Yu-Feng Wang
- Shu-Guang Hao
- Yi Ru
- She-Gan Gao
- Li-Dong Wang
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Affiliations: Department of Oncology, Cancer Institute, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Henan Key Laboratory for Esophageal Cancer Research, College of Medicine, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: April 2, 2013 https://doi.org/10.3892/etm.2013.1038
Pages: 1555-1558

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Abstract

The aim of this study was to characterize histochemical patterns and Das-1 and Ki67 protein expression in gastric cardia adenocarcinoma (GCA) and intestinal metaplasia (IM) lesions adjacent to GCA. Histochemical techniques, including Alcian blue/periodic acid-Schiff (AB/PAS), high iron diamine/Alcian blue (HID/AB) and avidin‑biotin‑peroxidase complex (ABC) immunohistochemistry were applied to GCA and IM samples from patients (n=200) in Linzhou, Henan, China, a high incidence area for GCA and esophageal squamous cell carcinoma (SCC). The detection rate of IM lesions in resected tissues adjacent to GCA was 32.5% (65/200). GCA and IM lesions presented a high frequency of Das-1 and Ki67-positive staining with statistical significance (P<0.01). The expression of sulfuric proteins did not show co-expression with Das-1 and Ki67 in GCA and surrounding IM lesions (P>0.05) from the same GCA patient. The high frequency of co-expression of Das-1 and Ki67 in GCA and adjacent IM lesions indicates that IM adjacent to GCA may undergo similar molecular changes to GCA, which may be one of the mechanisms for malignant transformation of IM in the population studied.

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1.	Abrams JA, Sharaiha RZ, Gonsalves L, Lightdale CJ and Neugut AI: Dating the rise of esophageal adenocarcinoma: analysis of Connecticut Tumor Registry data, 1940–2007. Cancer Epidemiol Biomarkers Prev. 20:183–186. 2011.PubMed/NCBI
2.	Guanrei Y and Sunglian Q: Incidence rate of adenocarcinoma of the gastric cardia, and endoscopic classification of early cardial carcinoma in Henan Province, the People's Republic of China. Endoscopy. 19:7–10. 1987.PubMed/NCBI
3.	Wang LD, Gao WJ, Yang WC, et al: Prelimary analysis of the statistics on 3933 cases with esophageal cancer and gastric cardia cancer from the subjects in the People's Hospital of Linzhou in 9 years. J Henan Med Univ. 32:9–11. 1997.(In Chinese).
4.	Tinmouth J, Green J, Ko YJ, et al: A population-based analysis of esophageal and gastric cardia adenocarcinomas in Ontario, Canada: incidence, risk factors, and regional variation. J Gastrointest Surg. 15:782–790. 2011. View Article : Google Scholar : PubMed/NCBI
5.	Wang LD: Mechanisms of human esophageal and gastric cardia multistage carcimegenesis. Zhonghua Zhong Liu Fang Zhi Za Zhi. 13:321–324. 2006.(In Chinese).
6.	Coupland VH, Allum W, Blazeby JM, et al: Incidence and survival of oesophageal and gastric cancer in England between 1998 and 2007, a population-based study. BMC Cancer. 12:112012. View Article : Google Scholar : PubMed/NCBI
7.	Fan YJ, Song X, Li JL, et al: Esophageal and gastric cardia cancers on 4238 Chinese patients residing in municipal and rural regions: a histopathological comparison during 24-year period. World J Surg. 32:1980–1988. 2008.PubMed/NCBI
8.	Gertler R, Stein HJ, Loos M, Langer R, Friess H and Feith M: How to classify adenocarcinomas of the esophagogastric junction: as esophageal or gastric cancer? Am J Surg Pathol. 35:1512–1522. 2011. View Article : Google Scholar : PubMed/NCBI
9.	Yamashita H, Katai H, Morita S, Saka M, Taniguchi H and Fukagawa T: Optimal extent of lymph node dissection for Siewert type II esophagogastric junction carcinoma. Ann Surg. 254:274–280. 2011. View Article : Google Scholar : PubMed/NCBI
10.	Sharma P, Weston AP, Morales T, Topalovski M, Mayo MS and Sampliner RE: Relative risk of dysplasia for patients with intestinal metaplasia in the distal oesophagus and in the gastric cardia. Gut. 46:9–13. 2000. View Article : Google Scholar : PubMed/NCBI
11.	Sugimura T: Multistep carcinogenesis: a 1992 perspective. Science. 258:603–607. 1992. View Article : Google Scholar : PubMed/NCBI
12.	Weinberg RA: How cancer arises. Sci Am. 275:62–70. 1996. View Article : Google Scholar
13.	Zhou Q and Wang LD: The studies on the biological characteristics of gastric cardia carcinoma. World J Gastroenterol. 6:636–637. 1998.(In Chinese).
14.	Chen H, Wang LD, Fan ZM, Gao SG, Guo HQ and Guo M: The comparison study of the three histochemical staining methods in gastric cardia intestinal metaplasia staining. Henan Med Res. 12:10–13. 2003.(In Chinese).
15.	Gao SG, Wang LD, Fan ZM, et al: Histochemical studies on intestinal metaplasia adjacent to gastric cardia adenocarcinoma in subjects at high-incidence area in Henan, north China. World J Gastroenterol. 11:4634–4637. 2005.PubMed/NCBI
16.	Mirza ZK, Das KK, Slate J, et al: Gastric intestinal metaplasia as detected by a monoclonal antibody is highly associated with gastric adenocarcinoma. Gut. 52:807–812. 2003. View Article : Google Scholar : PubMed/NCBI
17.	Yi XP: The advances in application researching on estimating prognosis of carcinoma with immunohistochemical method. Cancer Res Prev Treat. 23:382–385. 1996.(In Chinese).
18.	Das KM, Sakamaki S, Vecchi M and Diamond B: The production and characterization of monoclonal antibodies to a human colonic antigen associated with ulcerative colitis: cellular localization of the antigen by using the monoclonal antibody. J Immunol. 139:77–84. 1987.
19.	Zullo A, Hassan C, Romiti A, et al: Follow-up of intestinal meta-plasia in the stomach: When, how and why. World J Gastrointest Oncol. 4:30–36. 2012. View Article : Google Scholar : PubMed/NCBI
20.	Correa P: Is gastric cancer preventable? Gut. 53:1217–1219. 2004. View Article : Google Scholar : PubMed/NCBI
21.	Tang LJ: Review of research on gastric intestinal metaplasia. J Prac Oncol. 10:77–79. 1996.(In Chinese).