In vitro antibacterial activity of combinations of fosfomycin, minocycline and polymyxin B on pan‑drug‑resistant Acinetobacter baumannii

Zhang,Yajun; Chen,Fengzhe; Sun,Enhua; Ma,Ruiping; Qu,Chunmei; Ma,Lixian

doi:10.3892/etm.2013.1039

June 2013 Volume 5 Issue 6

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June 2013 Volume 5 Issue 6

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Article

In vitro antibacterial activity of combinations of fosfomycin, minocycline and polymyxin B on pan‑drug‑resistant Acinetobacter baumannii

Authors:
- Yajun Zhang
- Fengzhe Chen
- Enhua Sun
- Ruiping Ma
- Chunmei Qu
- Lixian Ma
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Affiliations: Department of Infectious Diseases, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Laboratory Medicine, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China
Pages: 1737-1739
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Published online on: April 2, 2013

https://doi.org/10.3892/etm.2013.1039
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Abstract

The aim of this study was to determine the effects of combinations of fosfomycin, minocycline and polymyxin B in the treatment of pan-drug‑resistant Acinetobacter baumannii (PDR-Ab). The in vitro antibacterial activities of the drugs were evaluated by determination of the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). A total of 25 strains of PDR-Ab were selected using the VITEK32 microbial analysis instrument and the Kirby-Bauer (K-B) method. A broth microdilution method was used to determine the MIC for each of the three drugs, and the checkerboard method was simultaneously used to determine the MICs for combinations of the drugs. FICI values were also calculated. While fosfomycin alone was ineffective for the treatment of PDR-Ab, its MIC value was significantly reduced when used in combination with minocycline or polymyxin B. The combined use of minocycline and polymyxin B also significantly reduced the MIC value of each drug. The FICI values revealed that the drugs had synergistic or additive effects when used in combination. The determination of the MIC and FICI values for the combinations of drugs demonstrated that there is synergistic or additive effect upon the combined use of fosfomycin with minocycline or polymyxin B. The combined use of minocycline and polymyxin B also results in a significant reduction in the MIC values of the two drugs. These experimental results may provide a basis for the future clinical treatment of Acinetobacter baumannii.

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1.	Shi Y and Liu DW: Exploration of treatment of multi-drug resistant Acinetobacter. Prescription Drugs. 9:11–16. 2006.(In Chinese).
2.	Liang W, Liu XF, Huang J, Zhu DM, Li J and Zhang J: Activities of colistin-and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients. BMC Infect Dis. 11:1092011. View Article : Google Scholar : PubMed/NCBI
3.	Lian JJ and Tang QY: Synergy and the clinical applications of fosfomycin as well as other antimicrobial agents. Foreign Medicine (Antibiotics). 1:44–50. 2003.(In Chinese).
4.	Pang XJ, Chen Y and Lu MD: Time difference attack therapy producing ESBLs G - 22 cases of bacillus infection. Chinese Journal of New Drugs. 3:351–352. 2005.(In Chinese).
5.	Pang XJ, Zhou HW and Wei H: Clinical studies of the treatment of Pan-drug resistant Acinetobacter baumannii infection. Chinese Journal of Pharmaceutical Sciences. 5:397–398. 2009.(In Chinese).
6.	Huang ZG, Huang C and Ma DY: Antibacterial activity of minocycline on Acinetobacter baumannii. International Journal of Laboratory Medicine. 10:1155–1157. 2010.(In Chinese).
7.	Shi YB and Dong Y: In vitro studies of tigecycline and polymyxin B treatments on pan-drug resistant Acinetobacter baumannii strains resistant to cefoperazone. Zhongguo weishengtaixue zazhi. 9:825–827. 2010.(In Chinese).
8.	Sopirala MM, Mangino JE, Gebreyes WA, et al: Synergy testing by Etest, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 54:4678–4683. 2010. View Article : Google Scholar : PubMed/NCBI
9.	Lee YS, Kang OH, Choi JG, et al: Synergistic effect of emodin in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. Pharm Biol. 48:1285–1290. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

In vitro antibacterial activity of combinations of fosfomycin, minocycline and polymyxin B on pan‑drug‑resistant Acinetobacter baumannii

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